We narrowed to 399 results for: SEF

1. Rett Syndrome: A History of Research and Therapeutic Outlooks

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   ...acquired skills such as speech, coordination, purposeful use of hands, and walking. They may exhibit slow...

2. To Codon Optimize or Not: That is the Question

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   ...interest? Codon optimization has become extremely useful in expressing functional proteins in hosts that...

3. Hot Plasmids - August 2020

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   ...evolution of other target proteins and might be useful for your studies.  Find the plasmids to set up ...

4. Data Freedom: The Expansion of Data Sharing in Research Publications

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   ...interest to ensure that our data are reproducible and useful to others and efforts to enhance these attributes...

5. Plasmids 101: Biotinylation

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   ...their solubility, and thus different reagents are useful for biotinylation in different microenvironments...

6. Quick Guide to Near-Infrared Fluorescent Proteins

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   ...near-infrared) (Qian et al., 2019).  NIR FPs are useful in vivo probes, due to low autofluorescence, low...

7. Adeno-associated Viruses (AAVs) for Genome Editing

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   ...for episomal gene expression and are particularly useful for gene delivery to the nervous system. For many...

8. March for Science

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   ...proceeds in an informed fashion. For science to be useful to governments, however, inconvenient data must...

9. Antibodies 101: Normalization and Loading Controls for Western Blots

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   ..., the notorious western blot: we meet again. So useful, yet so finicky to design and optimize. Today we...

10. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    ...played by microtubules, Opto-katanin will surely be useful for a wide variety of experiments. Opto-RhoGEFs...

11. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    ...plasmids described above, Addgene carries several useful CRISPR toolkits for creating plant expression plasmids...

12. Fluorescent Proteins 101: When GFP lets you down

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    ...fusion (Mastop, 2018). Summary GFP is an all-round useful genetically encoded probe, but there are limitations...

13. Evolution of Lab Techniques

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    ...largely replaced these, these techniques still have useful applications and have informed many current techniques...

14. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    ...the other long-homology based cloning methods are useful alternatives to the standard restriction/ligation...

15. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    ...junction. In zebrafish, the method has become very useful in lineage tracing; “Zebrabow” was used to trace...

16. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    ...able to apply someone else's new and incredibly useful technique simply by following the abbreviated methods...

17. CRISPR 101: Off-Target Effects

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    ...for every experiment, at least one will likely be useful. Optimal gRNA selection With some CRISPR experiments...

18. Plasmids 101: Restriction Cloning

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    ... concentration of recovered DNA as this will be useful for the ligation step. 3. Ligate Your Insert into...

19. Antibodies 101: Validation

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    ...antibody webpages. These outlets can be particularly useful for sharing negative data. Negative data often ...

20. 9 tips for a successful postdoctoral experience

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    ...'t like speaking, they don't feel like they get useful feedback, and they don't feel like they've made...