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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 661 results for: SIM;

Showing: 361 - 380 of 661 results

  1. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...over previous genome editing techniques is its simplicity and versatility. CRISPR/Cas9 consists of two ...Pooled lentiviral CRISPR libraries (referred to simply as “CRISPR libraries”) consist of a heterogeneous...

  3. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins from ...reporter cloned in. In these plasmids you would simply clone your gene of interest into the multiple cloning...

  8. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...for 8th graders was a little intimidating. Too simplistic and I would insult the students. Too complicated... mechanism, I was careful to keep it relatively simple. I honed the complexity of the talk by running ...

  9. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...sample(s) in the same buffer if possible, or very similar buffers if not, since buffer components can affect...only the linear portion. This allows you to use a simple linear regression trendline, which may be good ...

  11. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...to allow those who could not attend a meeting to simply stay caught up and to allow those who may be interested...own knowledge of the field. Perhaps interest has simply waned. When attendance slumps below a certain level...

  12. Plasmids 101: Colony PCR

    Type
    Blog Post
    ... insert and one primer anneals to the insert. A simple way to create this type of primer pair is to mix-and-match...  Primer Design Pros Cons Insert-Specific Simple "yes or no" test results Doesn't tell you the...

  14. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...to use the ICE software tool is quick and easy. Simply upload your Sanger sequencing files and provide...analysis, which compares hundreds of samples simultaneously.  The current version of ICE software can analyze...

  15. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...established partnership. If you’re willing to do similar things for your team or lab, you’re likely to be... of interacting and is much more effective than simply sending a message asking how things are going. ...

  16. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...in mammalian cells. SaCas9 produced indels at a similar efficiency to SpCas9, leading the group to focus...two proteins, mediates protein trans-splicing, similar to an intron in pre-mRNA splicing. In 2016, Fine...

  17. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...chalk talk? On the surface, a chalk talk is quite simple. It's you, a dry erase board, some markers (chalk...chalk talk Perfect your story and delivery. Design simple figures and diagrams to illustrate your vision....

  18. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...maintaining a stable concentration of plasmid. Similar to replication in E. coli, regulation primarily...replication of the plasmid proceeds (and terminates) similarly to the bacterial chromosome. Since replication...

  19. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...amenable to multiplexing; expressing 5 gRNAs simultaneously produces comparable gene knockdown to single...LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency but no significant off-target...

  20. Bioinformatics at Addgene

    Type
    Blog Post
    ...executed on Linux, we created our first process by simply copying and pasting pieces from our existing script...existing script into the section of the next process. Similar to our first attempt, we manually inspected the...
Showing: 361 - 380 of 661 results