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  1. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins from ...reporter cloned in. In these plasmids you would simply clone your gene of interest into the multiple cloning...

  3. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...for 8th graders was a little intimidating. Too simplistic and I would insult the students. Too complicated... mechanism, I was careful to keep it relatively simple. I honed the complexity of the talk by running ...

  4. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...sample(s) in the same buffer if possible, or very similar buffers if not, since buffer components can affect...only the linear portion. This allows you to use a simple linear regression trendline, which may be good ...

  7. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...to allow those who could not attend a meeting to simply stay caught up and to allow those who may be interested...own knowledge of the field. Perhaps interest has simply waned. When attendance slumps below a certain level...

  9. Plasmids 101: Colony PCR

    Type
    Blog Post
    ... insert and one primer anneals to the insert. A simple way to create this type of primer pair is to mix-and-match...  Primer Design Pros Cons Insert-Specific Simple "yes or no" test results Doesn't tell you the...

  12. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...to use the ICE software tool is quick and easy. Simply upload your Sanger sequencing files and provide...analysis, which compares hundreds of samples simultaneously.  The current version of ICE software can analyze...

  13. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...established partnership. If you’re willing to do similar things for your team or lab, you’re likely to be... of interacting and is much more effective than simply sending a message asking how things are going. ...

  14. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...to two fold for the final titer value, which is simply due to the noise of the assay. In other words, ...). However, because purified AAV preps contain similar and low levels of background protein and chemical...

  15. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... in different locations all over the world. But simply attending the conference is usually not enough....young professionals will never attend a job fair! Similar to a conference, job fairs bring you face-to-face...

  16. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...amenable to multiplexing; expressing 5 gRNAs simultaneously produces comparable gene knockdown to single...LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency but no significant off-target...

  17. How to Write a Scientific Review Article

    Type
    Blog Post
    ...Eventually I learned that it’s practical because there simply isn’t enough time to read every single paper. I...present it all in one place to get a sense of the similarities and differences among proteins in the class....

  18. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...infections, we are treating microbes with compounds similar to those they have been battling for centuries.... that will be more difficult to treat later. A similar situation can occur if you treat a bacterial infection...

  19. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...chalk talk? On the surface, a chalk talk is quite simple. It's you, a dry erase board, some markers (chalk...chalk talk Perfect your story and delivery. Design simple figures and diagrams to illustrate your vision....

  20. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...maintaining a stable concentration of plasmid. Similar to replication in E. coli, regulation primarily...replication of the plasmid proceeds (and terminates) similarly to the bacterial chromosome. Since replication...
Showing: 361 - 380 of 651 results