We narrowed to 665 results for: SIM;

...vectors can be used to tag endogenous genes and simultaneously introduce a selection marker for screening ... CRISPR/Cas Plasmids - Protein Tagging. Beyond simply monitoring protein localization and activity, CRISPR...

...Compared to humans and mice, flies have a relatively simple genome. They have four chromosomes: the X (and ... phenotypes, check out the Classical Genetics Simulator website. One interesting thing to know is that...

...figure below). The double-MMEJ strategy looks very similar to HDR, but it is accomplished using much smaller... of MMEJ. This double MMEJ strategy looks very similar to HDR, but it is completed using much shorter ...

...over previous genome editing techniques is its simplicity and versatility. CRISPR/Cas9 consists of two ...Pooled lentiviral CRISPR libraries (referred to simply as “CRISPR libraries”) consist of a heterogeneous...

...and dissemination of experimental protocols, simultaneously allowing scientists to actively comment, annotate...have a record of your work. This feature further simplifies lab work if you like to bring your phone in the...

...gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins from ...reporter cloned in. In these plasmids you would simply clone your gene of interest into the multiple cloning...

...forward-engineering the central dogma across many genes simultaneously. These parts have been carefully engineered...incredible hidden story here. The parts we are releasing simply plug into this existing economic activity. People...

...sample(s) in the same buffer if possible, or very similar buffers if not, since buffer components can affect...only the linear portion. This allows you to use a simple linear regression trendline, which may be good ...

...for 8th graders was a little intimidating. Too simplistic and I would insult the students. Too complicated... mechanism, I was careful to keep it relatively simple. I honed the complexity of the talk by running ...

...random association between FPs independent of PPIs. Simply compare the fluorescence intensity of your BiFC...whereas BiFC is useful in scenarios that call for a simple, yes-no answer — i.e., do two proteins interact...

...non-scientist friends and family is Part Five, called simply “Bad Science.” Zimmer kicks off the section saying...provides several. In Part Three of the book, called simply “Old Science,” he describes the now infamous tale...

...tiny bit of the sample itself — so easy that this simple procedure is often taken for granted. In this post... from a NanoDrop. The principles will apply to similar instruments from other companies, like the Implen...

...to allow those who could not attend a meeting to simply stay caught up and to allow those who may be interested...own knowledge of the field. Perhaps interest has simply waned. When attendance slumps below a certain level...

...discriminate between non-essential and essential genes similarly to the Brunello knock-out library while mitigating...between 150-75 nucleotides upstream of the TSS. Similar to the Brunello and Dolcetto library, sgRNAs were...

... insert and one primer anneals to the insert. A simple way to create this type of primer pair is to mix-and-match...  Primer Design Pros Cons Insert-Specific Simple "yes or no" test results Doesn't tell you the...

...doors. It would be a nightmare if we didn’t have a simple way to track all of our 40,000 samples available...  If a plate of samples is ever dropped, we can simply scan the tubes and know what is in each one. Even...

...maintaining a stable concentration of plasmid. Similar to replication in E. coli, regulation primarily...replication of the plasmid proceeds (and terminates) similarly to the bacterial chromosome. Since replication...

...in mammalian cells. SaCas9 produced indels at a similar efficiency to SpCas9, leading the group to focus...two proteins, mediates protein trans-splicing, similar to an intron in pre-mRNA splicing. In 2016, Fine...

...experimental purposes. But what now? MoClo really simplifies vector construction by combining restriction ...software includes Golden Gate cloning tools that simulate the restriction–ligation reaction. You can input...

...executed on Linux, we created our first process by simply copying and pasting pieces from our existing script...existing script into the section of the next process. Similar to our first attempt, we manually inspected the...