We narrowed to 424 results for: SUA

1. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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   Blog Post

   ... only around 400 amino acids in size. To give a visual analogy: if Cas9 or Cas12a is a soccer ball, TnpB...

2. QC Sequencing Technologies at Addgene

   Type

   Blog Post

   ...raw data.  Addgene’s de novo assembly pipeline usually generates the correct plasmid sequence. However...

3. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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   Blog Post

   ...basics of the process. Also refer to Figure 1 for a visual overview! Figure 1: General overview of ...

4. Bioinformatics at Addgene

   Type

   Blog Post

   ...Pandas, Numpy, and Biopython.  We started at the usual recommended place: documentation. We oriented ourselves...

5. Transferable Skills Guide: Creativity

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   Blog Post

   ...few scientists state that they’re not creative, usually saying that they’re not artists, or artistic, and...

6. 5 Reasons to Use Reddit for Science Communication

   Type

   Blog Post

   ...deliberately, cleverly, and secretly pissing people off, usually via the internet, using dialogue). Luckily r/Science...

7. The Challenges of Cell Culture

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   Blog Post

   ...is initially isolated. Regrettably this is not usually the case if you obtain a cell line from a colleague...

8. Hot Plasmids - August 2020

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   Blog Post

   ...Nanobodies are smaller in size, more durable, and usually much easier to clone, express, and purify compared...

9. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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   Blog Post

   ... would result in GFP fluorescence that can be visualized and sorted via flow cytometry. Figure 3...

10. An Inside Look into Addgene's Dev Team

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    Blog Post

    ...feature as done correctly. These initial stories are usually a result of collaborations between Addgene’s Product...

11. Transferable Skills Guide: External Collaborations

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    Blog Post

    ...are aware of a problem or a need for something, usually someone else has realized this too. Begin with ...

12. Designing Your Chalk Talk for the Academic Job Interview

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    Blog Post

    ... talk. Their first exposure to a chalk talk is usually their own. This is a problem. The chalk talk is...

13. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    Blog Post

    ...particles and establishment of virus-free hiPSCs usually require about 5-10 passages. Furthermore, working...

14. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post

    ...Harvard University.    Which titer do people usually report? Addgene and other viral vector manufacturing...

15. Transferable Skills Guide: Career Planning Resources

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    Blog Post

    ... world. But simply attending the conference is usually not enough. How do you stand out from the hundreds...

16. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    Blog Post

    ...analysis tools have been developed to help parse and visualize expression data in useful ways. Zebrafish mutants...

17. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post

    ...while the neuronal marker TUJ1 is useful for visualizing neuronal differentiation by immunohistochemistry...

18. How to Write a Scientific Review Article

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    Blog Post

    ...Finally, you can sound credible and cool when you casually mention “Haery et al., showed that MYC expression...

19. Plasmids 101: Stringent Regulation of Replication

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    Blog Post

    ...imposed on the host with a beneficial function (usually antibiotic resistance).  In the lab, low copy plasmids...

20. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post

    ...cloning purposes, these E. coli strains are not usually well suited for recombinant protein expression....