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Addgene

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We narrowed to 612 results for: Set

Showing: 361 - 380 of 612 results

  1. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...nature, they are largely underutilized in research settings — mostly a consequence of Cascade's complexity...

  3. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...antigen or an antibody depending on how the assay is set up. Then they block the capture reagent to prevent...binding to a primary antibody.  While the precise setup of the method can vary, typically users first coat...

  4. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...facilitates the use of prime editing in pooled settings with hundreds to thousands of pegRNAs. Chromatin-based...primer binding site, and many more. Once we had our datasets with all the different features and editing efficiencies...PRIDICT2.0 (Mathis et al., 2024). The additional datasets used in the training of PRIDICT2.0 make this model...

  5. Hot Plasmids - November 2023

    Type
    Blog Post
    ...This modular approach combines an interchangeable set of fusogenic and targeting components to reprogram...Every few months, we highlight a subset of the new plasmids, antibodies, and viral preps in the repository...

  7. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...and/or perceived professional risk involved in setting up a CURE, especially if it’s a new approach within... amenable to in-class labs. Provost ultimately settled on a two-semester model, in which students learn...

  9. CRISPR Antimicrobials

    Type
    Blog Post
    .... suggest that it could be valuable in medical settings where antibiotic resistant bacteria have previously...Even when researchers develop new antibiotics, the onset of resistance is swift, as few as five years after...

  12. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...independent variable. Biological replicates In any given set of experiments, the biological controls are more ...

  14. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...multiple genes is valuable in many experimental settings. To achieve this, scientists use a multitude of...individual expression cassettes, having the genes expressed from the same cassette is sometimes advantageous...and you can then insert the whole multicistronic cassette into a backbone as a single unit. Although it ...

  16. Video Library

    Type
    Protocol
    ...technique, including personal protective equipment, setting up a clean workspace, and maintaining sterility...

  20. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...gene-targeting capabilities of full-length SpCas9. This set of plasmids includes AAV-Cas9C-VPR for targeted gene...genes in human cells using compact expression cassettes. Sci Rep 5: . https://doi.org/10.1038/srep10777...
Showing: 361 - 380 of 612 results