Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 617 results for: Set

Showing: 361 - 380 of 617 results

  3. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ... used successfully in preclinical and clinical settings for ocular conditions. However, AdV was abandoned...responsible for an autosomal recessive, severe, childhood-onset form of blindness name Leber’s Congenital Amaurosis... of new capsid variants, designing expression cassettes to regulate and optimize transgene levels, and...

  4. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...mice CRISPRa has largely been limited to in vitro settings due to the large size of Cas9 activators, which...packaging limits (i.e., how many sgRNA expression cassettes will fit). Cre-dependent expression of the SPH...

  5. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...the day of your chalk talk Bring your own fresh set of erasable markers. You’ll have the colors you want...research? Do you have innovative tools? A novel dataset? Unique expertise? A novel hypothesis? Part of ...

  6. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ... the use of a given timer in many experimental settings. Monomeric fluorescent protein timers Since the... simplifies cloning and makes your experimental setup less cumbersome. 3. Portability across multiple ...

  7. MXS Chaining

    Type
    Blog Post
    ...sequences of the genetic elements may differ (e.g., a set of various mammalian promoters), the same cloning...sequences. Intermediate chaining products (called “cassettes”) can be readily reused in the assembly of any...any later construct (i.e.,functional cassettes are recyclable) Can only work if the restriction sites used...

  9. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...have become a powerful tool to address a diverse set of biological inquiries. References Ogura, Teru...death. The team used this cassette to knock out genes, knock in a GFP cassette, and introduced site-directed...for Zeocin resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct repeats which can be ... selects for those cells that have removed the cassette through a second recombination event between the...

  11. CRISPR Antimicrobials

    Type
    Blog Post
    .... suggest that it could be valuable in medical settings where antibiotic resistant bacteria have previously...Even when researchers develop new antibiotics, the onset of resistance is swift, as few as five years after...

  14. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...independent variable. Biological replicates In any given set of experiments, the biological controls are more ...

  15. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...multiple genes is valuable in many experimental settings. To achieve this, scientists use a multitude of...individual expression cassettes, having the genes expressed from the same cassette is sometimes advantageous...and you can then insert the whole multicistronic cassette into a backbone as a single unit. Although it ...

  17. Video Library

    Type
    Protocol
    ...technique, including personal protective equipment, setting up a clean workspace, and maintaining sterility...

  18. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...gene-targeting capabilities of full-length SpCas9. This set of plasmids includes AAV-Cas9C-VPR for targeted gene...genes in human cells using compact expression cassettes. Sci Rep 5: . https://doi.org/10.1038/srep10777...

  19. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...your “gating strategy.” This series of markers are set up sequentially, from broadest to most specific marker...Subtypes of immune cells are often defined by a subset of the markers used to identify them, such as “...
Showing: 361 - 380 of 617 results