We narrowed to 731 results for: Sis;
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TypeBlog Post... dynamic process which involves autophagosome synthesis, delivery of materials to be degraded to the lysosome...mammalian dual-emission autophagy biosensor. Its resistance to lysosomal proteases is key to its pH-dependent...
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Plasmids 101: Origin of Replication
TypeBlog Post..., 2020. Now that we know all about antibiotic resistance genes, let’s consider another basic element of...for single- and multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post...We believe that this simplified sequencing and analysis approach can be easily incorporated into standard... A Rapid and Robust Assay for Batch-to-Batch Consistency Evaluation of AAV Preparations. Molecular Therapy... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...University of North Carolina (plasmids at Addgene). It consists of two domains, LOV2 and Zdk1, where Zdk1 binds...RNA localization in mammalian cells. These tools consist of cobalamin-binding riboswitches that bind to ... -
Summer SciComm Series: Master of SciComm
TypeBlog Post...perception of head injury risk. In completing a master’s thesis in science communication, I had the chance to carry...necessary to inform the experimental aspect of my thesis: a survey instrument designed to measure how individuals... -
Intro to Our Summer SciComm Series!
TypeBlog Post...Instead, it targets a specific audience - giving consistency, purpose, structure, and intent to the messaging...your audience, and their expectations of you, consistent and clear. And now that we’re all on the same... -
Antibodies 101: Epitope Tags
TypeBlog Post...detection of the tagged protein. These peptides consist of as little as 8 or as many as several hundred...10.1007/s00253-002-1158-6 Baker, M., Reproducibility crisis: Blame it on the antibodies. Nature (2015) 521,... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog Post...degree and some marketing experience, with little emphasis placed on the marketing or business background...thought they would focus on “Has this person been consistent throughout their career?”, "Have they sought ... -
Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog Post...activity to start a discussion. The Forced Choice Analysis is a fun example and you can find it in our Mentoring...series of mentoring blog posts, the "Forced Choice Analysis Activity", the "Peer Mentor Groups Guide", "Tips... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog Post...modeling, directed evolution, site-saturation mutagenesis, and medium-throughput screening, she increased... plant surfaces. His system of choice is the Arabidopsis thaliana - Pantoea agglomerans interaction. But... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...visualized and results were consistent with copy number variation (CNV) analysis of the U2OS cells. ... -
Addgene Depositors Get More Citations
TypeBlog Post...controlling for journal. And so, while our quantitative analysis cannot rule out all other explanations, we do ...ssrn.com/author=1232466. Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT... -
Science Career Options
TypeBlog Post...Ecologist Computer and writing skills Writing a thesis or dissertation demonstrates that you have advanced...what each of these roles involves on a dad-to-day basis. Often, it is hard to know what a job title actually... -
The MTA at Addgene
TypeBlog Post...beginning makes the sharing process smoother, more consistent, and more pleasant for everyone, which in turn...including MTAs, is perfect, but it does provide consistency and transparency in an otherwise messy process... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...2017. Pubmed PMID: 16371404 Sniderman et al, Athersclerosis 2012. Pubmed PMID: 23068583 Thierer et al, ...UAS). The Wang Lab created a tripartite system consisting of dCas9, a gRNA fused to an RNA binding motif... -
Advice for Scientists Starting a Lab
TypeBlog Post... prepared me for what a PI without a personal assistant has to deal with. My inbox currently stands at...advisor Wally Gilbert: “Very interdisciplinary, with emphasis on genetic engineering, sequencing, and translation... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post...probable mature human miRNAs. Weiss’s collection consists of a library of reporter plasmids, each containing...However, a CRISPR-based tool for in vivo functional analysis of immune cells has been a gap in the field. Arlene... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog Post...integrate a reporter gene that contains a puromycin resistance gene followed by the target site (the same sequence...: A donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP. This visual... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post...flies? Sure, the vector will have an antibiotic resistance gene to select for transformed bacteria, but ...Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila midgut. Cell. 137, 1343–55 ... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...this multi-pronged attack. Inflammation and tumorigenesis The conference opened with a very interesting...highlighted the UCSF Immunoprofiler - a human biopsy analysis platform focused on analysing immune measures ...