...: Čelešnik, Helena, et al. "Biosafety of biotechnologically important microalgae: intrinsic suicide switch..., Xuechen Zhu, and Charles E. Melançon III. "Detection and Quantification of Ribosome Inhibition by Aminoglycoside...fusions reported in the literature include RNA detection, RNA cleavage, and translational repression or...Genetically Encodable System for Sequence‐Specific Detection of RNAs in Two Colors."ChemBioChem 17.10 (2016...

...with antiviral therapies such that the virus is undetectable in the blood. But the virus doesn’t completely...respond. Latently infected cells evade immune detection because they produce very little or no viral protein...reinfection. Importantly, no off-target effects were detected. Kaminski et al. observed no negative effects ... field, the lessons learned in pursuing one technological application can benefit many others and accelerate...

... IMP USA-Europe not only provide the specific technical and logistic support to increase the chances of...of success, but also nurture a supportive and protective environment where individuals are empowered to...represented a diverse group of individuals with technical and career path needs, and came from the universities...Harvard University, Massachusetts Institute of Technology, Boston University, Johns Hopkins University,...

...Table 1: AAV Serotypes and Tropism Experiment  Detection Method CNS AAV1, AAV2, AAV4, AAV5, AAV8, AAV9...purposes. The University of Pennsylvania and the biotechnology firm Biogen have recently collaborated to develop...is their ability to be used with new exciting technologies (such as CRISPR/Cas9) as they allow for gene... fourth generation of neuroanatomical tracing techniques: exploiting the offspring of genetic engineering...

...HaloTag or SNAP-tag construct can help you better detect dim structures or low levels of expression with...performance and use super-resolution or single-molecule techniques.   Figure 2: Two-color super-resolution...proteins with small molecules in vivo. Nature Biotechnology, 21(1), Article 1. https://doi.org/10.1038/nbt765... V. (2008). HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis. ACS Chemical...

...scientific team came as an undergrad at Worcester Polytechnic Institute. Though some WPI students chose to ... physical kits are made and shipped; Chiara, the tech transfer expert who understands the somewhat complicated...repository, performing quality control, and providing technical customer service. Team members are highly trained...a biostatistician, and that one golden-handed technician who can do a certain difficult assay with her...

... label it with finesse using tRNA suppression technology (Fig. 1) (Dumas, Lercher, Spicer, & Davis, 2015... from depositing labs that have mastered this technique so it might be worth a try, especially since the...References 1. Degorce, François, et al. "HTRF: a technology tailored for drug discovery–a review of theoretical...Time-Resolved FRET-Based Approach for Antibody Detection–A New Serodiagnostic Concept." PloS one 8.5 (2013...

...Addgene gave to me: SHERLOCK and DETECTR, two CRISPR-based methods to detect nucleic acids.  On the third ... CRISPR, Addgene gave to me: five methods for detecting SARS-CoV-2 using CRISPR.  On the sixth day of ...

... themselves. All of them have cool new tools, techniques, or information that is now available for other...demethylation. AAV Q&A with Tim Miles: While not technically a research story, I couldn't resist adding in...learning about it (and maybe even picked up a new technique or tool to try in your own work!) If you've got...

...particular microorganism, microbial technique, or microbial technology, sign up to write about it on the...CRISPR guide to find out how to use genome editing techniques in your microbe of choice. For more help with...

...using the streak plate technique. Time for the #BioSci3319 #StreakPlateChallenge part 1. Lab B needs a...Sciences at Missouri University of Science and Technology, has been teaching the microbiology lab every...

...CRISPR continues to grow as the genome editing technique has developed and improved. The CRISPR posts written... for more detailed information about this new technology. Our Plasmids 101 series continues to grab scientists...questions or if you have a topic, researcher, or technology you would like us to feature in the coming year...

...got to learn about intellectual property from our Tech Transfer team members Tony Cova and Chiara DeNatale...DeNatale and programming with our Chief Architect Pam McA' Nulty (check out our blog post on CRISPR software...presentation on the emerging CRISPR genome editing technology was a rousing success! Many of the students were...

... in skeletal muscle by exercise and endurance, protects the brain from depression. Mice that are genetically...stressors as usual, but somehow their brains were protected. Effects of Activation of the PGC-1a1 Pathway ...levels of kynurenine in the body, the brain is protected from stress-induced biochemical changes that could...

...them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented an exciting way of listening to microbes...vesicles) as acoustic biomolecule-based dyes. This technology is widely applicable as bacterial expression ... high spatial resolution are benefits of this technique. It is even possible to multiplex ultrasound dyes...

...perturbations – in the case of screens using CRISPR technology, gRNAs to knockout genes – are essential screening...the validity of your biological model and the technical performance of your assay. But, in many discovery-oriented... off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed Central...

...are also the foundation of multiple important technologies, including FRET and optogenetics. Even though...photoconvert GFP from green to RED! This simple technique has been shown to work in plant, Drosophila and...protonated form of GFP is photoconverted, so this technique may not be appropriate for high-pH cellular compartments...

...career goals, and celebrate accomplishments. These techniques are laid out in Vincent et al., and are easily...a recap of accomplishments, from learning new techniques to finally figuring out a tough experiment, sets... in every lab, but the principles behind this technique are beneficial for researchers at all stages. ...

...inner beauty of familiar flowers." This new x-ray technique provides scientist with a new method to explore...by Darwin on his voyage. Previous destructive techniques were incompatible with the need to conserve these...Richards’ work is held by The Museum of Jurassic Technology in LA, where it is currently on display.   Many...

...service. A small subset of AAV plasmids will have technical or legal restrictions that make them ineligible...be reviewed by Addgene for legal approvals and technical compatibility with our production process. This...found in our in-stock preps. If a request has technical considerations that may affect expected titers...