...preclinical and clinical data about a disease or therapeutic Side-by-side comparison of the same experiment...reproducible. For an example, see our ReFigures on the therapeutic use of anti-CD47 antibodies in immuno-oncology...

...and digital articles where it’s easier to give attribution, than for journal publications. Google Image ...includes images that can’t be reused without attribution, despite filtering for images labeled for reuse...

...bioluminescence. Substrate pharmacokinetics and biodistribution therefore introduce additional confounding ...have deep tissue penetration and high spatial resolution. By piecing together genes from gas vesicle operons...

... improving expression of nutritionally and therapeutically significant proteins. In this project she aims...Expression Systems Learn about Global Plasmid Distribution ...

...bosses tell people where they stand clearly and routinely. They are clear with each employee about what ...easier with practice so you must build it into your routines. This is the third in a 5 part series on Management...

...out to science writers in your area or at your institution; the news or communications office is a good ...internship at a place like Science News. Ask your institutional news office if there might be opportunities ...

...being appreciated on an aesthetic level as well. Beautiful microscope images which traditionally languished...on blossoms, thus revealing the startling inner beauty of familiar flowers." This new x-ray technique ...passion for art and science? Do you look for the beauty in the microscopic organisms on your lab bench?...

...peptide (YPYDVPDYA) derived from human influenza hemagglutinin. The tag has two negatively charged amino acids... a few instances when they should be used with caution or not at all. The introduction of a tag at the...

...selection of transformed plants to decrease the routine usage of antibiotics which may contribute to the...Chapel Hill. He has a passion for microscopy and beautiful imaging.   References and Resources References...

...frustrating for solo flyers Other people will routinely scrutinize your data—science can be surprisingly more...

...for Enhanced CRISPR Genome Editing CRISPR has revolutionized how researchers make knockouts and other types...fantastic depositors and guest writers. Your contributions make Addgene and the Addgene Blog useful resources...

...scientist in Beijing, talking to people about taking beautiful photographs of their studies, reporting on amazing...dialogue specifically about the project and contributions, rather than getting defensive or getting into...

...assay in the mouse intestine, demonstrating its utility in long-term tracking in vivo (Guo et al., 2019... induced pluripotent stem cells or the clonal evolution of cancer. If you’re looking to use CellTag, you...

...is driven by the dedicated community of data-contributing researchers who use AAV tools. This word...opportunity to cite the image to give proper attribution.  We hope that this provides a clear overview...

...variety of journals. Since then, I developed a routine that works well for me to evaluate the submitted...general, I think that this is an (e.g. great contribution to the community because of..." This first couple...

...quite honestly, does not value your potential contribution to the healthcare landscape.  You will find ...partnerships with universities, companies, hospital institutions and funding bodies. Don't shy away from the ...

...close enough to cause a DSB. The nickase system is utilized in many CRISPR systems to achieve specific genetic... in nature, but have been created by directed evolution of the Escherichia coli TadA, a tRNA adenosine...window. These systems are well-suited to directed evolution applications. Examples of these base editing systems...activity. Knockout libraries often target 5′ constitutively expressed exons, while activation and repression...repression, many CRISPR epigenetic tools require constitutive expression of the dCas9 fusion and/or expression...CAPTURE system, developed by the Jian Xu lab , utilizes biotin tagging of dCas9 by fusing a biotin acceptor... Kim, D., Kim, S., & Kim, J. (2018). Directed evolution of CRISPR-Cas9 to increase its specificity. Nature...

... factor 1 alpha CAG Constitutive Strong hybrid mammalian promoter PGK Constitutive Mammalian promoter ...Description T7 Constitutive but requires T7 RNA polymerase Promoter from T7 bacteriophage Sp6 Constitutive but requires...Research Promoter Expression Description CMV Constitutive Strong mammalian promoter from human cytomegalovirus...Inducible Tetracycline response element promoter U6 Constitutive Human U6 nuclear promoter for small RNA expression...polymerase Promoter from Sp6 bacteriophage lac Constitutive in the absense of lac repressor (lacI or lacIq...

... Rep68 to initiate replication and a terminal resolution site that serves as the target site for Rep proteins...structure. The Rep binding element (RBE) and terminal resolution site (trs) are noted. Created with biorender....

...enabling everyone to participate in the biotech revolution. Our lab exists in what appears to be a funky...proof-of-concept experiments for discovering novel therapeutics. Dave is a chemical biologist with a Ph.D. from...