...volume = more cells = more plasmid. Plasmids can vary in copy number, the number of plasmid copies supported...plasmid DNA yield. Different plasmids and strains will vary in their optimal growth conditions. For many high...cell density or by harvesting and miniprepping at various time points. Measuring the optical density of the...

...discovered that there are many ways to get a taste of a variety of careers while still working towards your degree...graduate office arranged opportunities to meet with various professionals, and there were multiple avenues ... Find Plasmids for Your Research Learn About A Variety of Different Fields on Our Collections Pages ...

...Technology Development Fellow at the Wyss Institute and Harvard Medical School. Scientists making transgenic organisms...seeks to keep the organisms in the laboratory. It varies by organism, but your local biosafety officer should... cas9 gene). An immunizing reversal drive is a variant that also spreads through the wild population and...

...one used in Luxturna™, can efficiently transduce various types of retinal neurons. Nowadays a toolbox of...serotypes are currently used in clinical trials, a variety of engineered ones have shown great promise in ...improve the technology: engineering of new capsid variants, designing expression cassettes to regulate and...

...infection and screening by PCR enrich for capsid variants with increased ability to infect the cell type...transduced with multiple viral particles and labeled a variety of colors (figure 4b). But notice in figure 4b ... BP, et al. Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain...

...PspCas13b-ADAR2DD(E488Q) displayed robust editing with various spacer lengths from 30-84 nucleotides, and designated...targeted 34 pathogenic G->A mutations from the ClinVar database. They successfully edited 33/34 sites ...in therapeutic applications, the authors tested various dRanCas13b truncations that would allow packaging...

... new cutting edge science, researchers of all varieties must stay current with literature and emerging...clear that while several people across teams had various levels of experience with bioinformatics and HTS...welcome to ask any questions they have.  These variations are suited for different applications and should...

...10% of DNA repair, with this rate varying across cell types. Variance can be caused by cell cycle rates...mutated in cancer cells, specifically in breast and ovarian lines commonly used in lab. It is recommended you...

...scientists understand how our vectors perform in various experimental settings. It is a curated set of technical...the knowledge base of AAV vector performance in various brain regions and cell types. Power in numbers ...Boulis, N. M., & Donsante, A. (2019). Lot-to-Lot Variation in Adeno-Associated Virus Serotype 9 (AAV9) Preparations...

...lacZ reporter gene (3, 4), there are now many variations, including many that use auxotrophic rescue genes...provides a baseline level of reporter activation. Vary the expression levels of bait and prey proteins ...your protein are screened for binding. Using a variety of expression levels and systems can alleviate ...

...researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out... people outside the department to accomplish a variety of goals. For example, I worked with some microscopy...current job at Addgene I work with people from various organizations in science and beyond. For example...

... knowledge they require. Biotechnology companies vary to what extent they hire experts for their technical...complain about high background. In many instances, variations of the product’s specific application instructions...support roles A job in technical support can add a variety of skills to your CV and lead to other job opportunities...

...use Gateway to clone a gene of interest into a variety of expression systems. Keep reading to learn more...ready-made entry clones can be used with a large variety of plasmids. Speed The Gateway system enables the...Addgene. These can be used to express genes in a variety of model organisms. Use the links below to find...

...still waiting for an indication of what (most of) Harvard, Johns Hopkins, (most of) MIT and the University...sciences. The Dana Farber Cancer Institute (at Harvard) and MIT BCS (Department of Brain and Cognitive...Boston Children’s Hospital is the only part of the Harvard group that has responded to the FLSA at the time...

...endogenous Uracil DNA N-glycosylase (UNG) enzymes. Various other proteins included in different CGBE tools... efficiency, purity, and indel rate of each tool vary considerably. And there’s so much more exciting ...models-assisted optimization of a uracil-N-glycosylase variant enables programmable T-to-G and T-to-C base editing...

...researchers feel they lack formal training in a variety of transferable skills. At Addgene we're helping...5 mental mistakes that kill your productivity. Harvard Business Review. Retrieved January 14, 2022, from...brief exercise to spur innovation on your team. Harvard Business Review. Retrieved January 14, 2022, from...

...al., 2013, Cho et al., 2014). There are several variants of the CRISPR/Cas9 system floating around, and...and many pre-designed plasmids containing these variants ready for purchase. But what is the easiest and...mouse lines that could be used to study a wide variety of phenomena, it’s possible that we’ll learn much...

...cell lines can become cross contaminated and are invariably much older than those available from biorepositories...thaw a new batch. The exact number of passages can vary between cells lines, as this can be dependent on...culture research experience through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune...

...inert green, cyan, yellow, and blue variants that can be used in various combinations. For example, with ...one of the most inert solutions for imaging in a variety of cellular compartments including the secretory...

... Mammalian Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan...Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange... mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly used with Clover pGWF1 Cyan & Yellow...