Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 1,091 results for: addgene

Showing: 361 - 380 of 1091 results

  2. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

    Type
    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...

  3. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ... Science Training Resources on Addgene.org Check Careers at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...small to mid-sized companies and organizations. Addgene as a nonprofit organization is very considerate...the community of biological scientists who use Addgene everyday. To get this and other projects like it... it going, someone (likely several people at Addgene) had to take the initiative to find a suitable partner...outside your comfort zone As part of my position at Addgene I am now involved in Strategic Partnership Management...collaborations is important? In my current job at Addgene I work with people from various organizations in...

  4. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...Additional Resources on the Addgene Blog Important Considerations When Using AAVs Addgene's AAV Quality Control...performed on all new viral vector preparations. Addgene has used this method to generally validate our ...   Figure 1: Electron micrograph of an Addgene AAV viral vector preparation after negative staining...    Which titer do people usually report? Addgene and other viral vector manufacturing facilities...compared.   Figure 2: Label that comes on Addgene’s AAV aliquots. Our titer values (circled in red...improvement over qPCR (Taylor et al., 2017). What do Addgene AAV titers mean for your experiments? Because (...AAV in vivo to determine the optimal dosage. At Addgene, we don’t reserve lots, but if you’re reordering...

  5. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...achieve temporary repression of protein expression. Addgene has a dCas9 KRAB plasmid for transient knockouts...Want to transiently induce expression? Check out Addgene's Lentiviral dCas9 SAM vector and Piggybac dCas9...on/off promoters and modes of action Check out Addgene's Lentiviral Tet-on and AAVS1 targeted Tet-on transgene...degradation. Adapted from Nabet et al. Check out Addgene's Lentiviral dTAG cloning vector and SMASh degron...PubMed PMID: 29581585. Additional resources on the Addgene blog Plasmid 101: Inducible Promoters Plasmid...for my Experiment Additional resources on the Addgene website Promoter Guide CRISPR Guide Lentiviral...

  6. Clear and Accessible Writing

    Type
    Blog Post
    ...Addgene was built on the concept of accessibility: making plasmids more accessible to scientists around...the next twenty years. But learn we did! From Addgene's very beginning, we had to consider digital accessibility... accessibility on our website.  As members of Addgene’s content team, we — Jo, Brook, and Angela — most... in a poster compared to an online abstract or Addgene blog post.  Here, we are presenting this image ... with the rest of the workshop.  For Figure 1, Addgene chose this alt text: "The left panel is a profile...writing!   Resources Additional resources on the Addgene blog Five Steps to Writing Grant Proposals to Grad...

  7. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    ..., and that means it's officially the start of Addgene's winter holiday celebrations! We're kicking it ...Joachim Goedhart  Created in collaboration with Addgene writers High Complexity Golden Gate Assembly with...big thank you to all who have contributed to the Addgene blog this year! Your contributions have helped ...guest authors, while others chose to work with Addgenie writers to help share their research, tools, and...

  8. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ... your research Subscribe to the Addgene blog Resources on Addgene.org Learn about the Michael Davidson...Allele Biotechnology that now can be deposited at Addgene. What makes these fluorescent proteins unique and...scientific community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "Directed evolution...PMCID: PMC3811051. Additional resources on the Addgene blog Read our fluorescent protein blog posts Find...Davidson fluorescent protein collection Check out Addgene’s fluorescent protein plasmids and resources Find...

  9. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ...options Resources on Addgene.org Check out Addgene's job openings Find eBooks from Addgene, including the ...development tips, take a look at the Addgene Science Career Guide eBook Addgene Science Career Guide eBook.   ...   Resources Resources on the Addgene blog Learn about career planning resources What Do I Do Now? ...

  10. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...have probably heard about Addgene’s viral service but did you know that Addgene also performs independent...101 eBook Additional resources on Addgene.org Learn about Addgene's viral service Learn about viral production...research? Since launching the viral service in 2016 Addgene’s research team has been actively investigating ...PMCID: PMC2957237. Additional resources of the Addgene blog Read our beginner's guide to viral vectors...

  11. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...Natronobacterium gregoryi, or NgAgo for short. Addgene has already distributed this plasmid all over the...supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and NgAgo head to head NgAgo has a number ...27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan C., et al.  “DNA-guided DNA interference...PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, Daan C., et al. “Argonaute of the ...PMCID: PMC4446448. Additional Resources on the Addgene Blog Read About Cas9 Homolog Cpf1 Check Out High...Genome Engineering Posts Additional Resources on Addgene.org Check Out Other Argonaute Technologies from ...

  12. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...ready to request from Addgene so you can combine the sensitivity of HiBiT with Addgene's quality control, ...Collection that express similar constructs available at Addgene. Read the original paper for more detail on whether...disruptive tag. Find HiBiT-TP53 Fusion Vector at Addgene! Following extracellular RNA editors Tracking factors...flow via EVs. Find ADAR-HiBiT Fusion Vector at Addgene! Watching protein degradation in real time Proteins...measurements. Find CFTR-HiBiT Fusion Vector at Addgene! Real-time tracking of receptor internalization...Find HiBiT-EGFR and EGFR-HiBiT Fusion Vectors at Addgene! Measuring messengers: growth factors Growth factors...receptor complex. Find sHiBiT-KDR Fusion Vector at Addgene! HiBiT advances detection of endogenous proteins...

  13. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ... deposited the plasmids used for this study at Addgene which include a C-terminal deletion in the spike...containing these mutations have been deposited at Addgene. Plasmids deposited from Alejandro Balazs’s lab... Bst polymerase constructs from their assay to Addgene.  Drew Endy’s and Philippa Marrack’s labs also ...investigate how the virus interacts with human cells. Addgene’s mammalian target plasmids express these and other...Brunello (knock out) and Calabrese (activation), from Addgene to better understand which human genes are important...10.1089/crispr.2020.0030 Additional resources on the Addgene blog Learn more about mapping the SARS-CoV-2 -... Plasmids & Resources page Stay up-to-date on Addgene's operations during the pandemic Find SARS-CoV-2...

  14. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...plasmids from this paper at Addgene. Additional Resources on the Addgene Blog Check Out Our CRISPR Featured... PMC5127784. Find plasmids from this paper at Addgene. 2. East-Seletsky, Alexandra, et al. "Two distinct...PMID: 27669025. Find plasmids from this paper at Addgene.  3. Gootenberg, Jonathan S., et al. "Nucleic ... PMC5526198. Find plasmids from this paper at Addgene. 4. East-Seletsky, Alexandra, et al. "RNA Targeting...: 28475872.  Find plasmids from this paper at Addgene.  5. Abudayyeh, Omar O., et al. “RNA targeting...PMID: 28976959. Find plasmids from this paper at Addgene. 6. Shmakov, Sergey, et al. "Discovery and functional...Multiplex Genome Editing with Cpf1 Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs...

  15. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...26411297. Resources at Addgene Find CRISPR plasmids at Addgene Read Addgene's CRISPR Resource Guide View...26422227. Find plasmids from this publication at Addgene. 2. Zetsche, Bernd, et al. "Multiplex Gene Editing...27918548. Find plasmids from this publication at Addgene. 3. Kim, Daesik, et al. Genome-wide analysis reveals...27272384 Find plasmids from this publication at Addgene. Kleinstiver, Benjamin P., et al. "Genome-wide...27347757. Find plasmids from this publication at Addgene. Makarova, Kira S., et al. "An updated evolutionary...

  16. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...handful of Click Biology tools available at Addgene. Addgene Link Click Biology Tag Description Humberg...by dialing up the right nucleic acid sequence (Addgene can help with this!). Besides requiring stable ...signaling. Many of these tools can be found in Addgene's repository (Table 1). Figure 2: Adding .... Many of these tools are already deposited at Addgene in multiple formats: for use in different organisms...s41589-025-01944-x   Additional Resources on the Addgene Blog Live and Let Dye: Self-Labeling Protein Tags...Plasmids 101: Degron Tags Additional Resources on addgene.org New England Biolabs Cell-Imaging Plasmid Collection...

  17. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...guide to chemogenetics Resources on Addgene.org Check out Addgene's chemogenetics guide Find chemogenetics...and subsequently another round of signaling.    Addgene Tools for Studying GPCR signaling  So with the ...look at a few examples with plasmids deposited at Addgene.  PRESTO-TANGO: The ligands that activate many...GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth’s lab also assembled a collection...table of DREADDS and their activity in neurons on Addgene’s chemogenetics guide.     References and resources...s0014-5793(02)02775-8 Additional resources on the Addgene blog Learn more about TRUPATH Read our quick guide... Various Addgenies...

  18. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...plasmids from this publication at Addgene. Resources on the Addgene Blog Read about Cas9 Homolog Cpf1...-HF1. The HypaCas9 plasmids are available from Addgene here! Future possibilities for Cas proteins With...26628643. Find plasmids from this publication at Addgene. 2. Nishimasu, Hiroshi, et al. "Crystal structure...26735016. Find plasmids from this publication at Addgene. 5. Chen, JS, et al. "Enhanced proofreading governs...Browse Our CRISPR 101 Series Resources on the Addgene Website Find CRISPR Plasmids Browse our CRISPR...

  19. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ... hear from Addgene’s former blog editor Tyler Ford on how initiative landed him at Addgene. What is initiative... Once in-person, peer-mentoring groups at Addgene have gone virtual!   2. Practice public speaking...almost every career talk I present, I’m asked what Addgene “looks for” when hiring a scientist. Of course,... comments below! Additional resources on the Addgene blog Read our science career blog posts Check ...

  20. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...Find plasmids from this paper at Addgene. Additional Resources the Addgene Blog Learn More about SunTag... remained elusive. Two techniques developed by Addgene depositors have made it easier to track translation...PMC2746483 Find ribosome profiling plasmids at Addgene. 2. Halstead, James M., et al. “Translation. An...: PMC4451088 Find plasmids from this paper at Addgene. 3. Yan, Xiaowei, et al. “Dynamics of Translation... Brightest Fluorescent Protein Resources on Addgene.org Find Fluorescent Protein Plasmids Check Out ...
Showing: 361 - 380 of 1091 results