...molecular biology in our Plasmids 101 eBook! Researchers use various techniques to control protein expression..., biotechnological, and medical applications. Researches can visualize proteins in vivo by tagging them... also be purified for use in molecular biology research (eg. polymerases and other enzymes might be purified...types of plants such as maize, tobacco, rice, sugarcane, and even tubers of potatoes have been used for...and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins under the control...Resources on Addgene.org Bacterial expression systems Search for plasmids by expression system ...

... more than 10 modules often rely on two-step hierarchical approaches using different Type IIS restriction...Development of Golden Gate Assembly test systems Our research focused on three different levels of assembly:...number of transformants. To compensate for this, researchers can plate larger volumes of the outgrowth containing... to minimize this possibility. Recently, NEB researchers profiled the comprehensive fidelity of cohesive...important tools for many areas of science, and researchers continue to test the limits of DNA assembly approaches...and bias during DNA end-joining." Nucleic acids research (2018). PubMed PMID: 29741723. PubMed Central ...

... in humans such as measles, mumps, and rabies. Research on Ebola virus has been limited, in part, due ... virus (EBOV) are extremely useful, allowing researchers to study aspects of the EBOV replication cycle...Finally, having four separate plasmids allows researchers to partially control expression levels of each...as the one described here, are extremely useful research tools. In addition to the reduced requirements...biocontainment precautions, these systems allow researchers to investigate viral processes in extreme detail...stranded hemorrhagic fever viruses." Antiviral research 91.2 (2011): 195-208. PubMed PMID: 21699921. PubMed...

...request activity around March. A rough estimate shows that activity level in March was around 33% of that... the rest of the world, from mid-January to mid-March.  To obtain a better view of how plasmid orders ...our distribution, resulting in the following hierarchical model: Because the U.S., Europe, and China ...University. Max Shen is a Ph.D. candidate at MIT. His research uses applied machine learning and statistical ...Howard Hughes Medical Institute Investigator. Liu’s research integrates chemistry and evolution to illuminate...

...the term ‘grant writer’, but make no mistake, researching, winning, and effectively managing grants involves...served as a principal investigator for several research grants. One of the benefits of being a principal...niche as a grant consultant to faculty writing research grants, especially in the sciences and by extension...your existing skills & passion  Skills such as research, collaboration across multiple disciplines, writing...shelf raise money? Do you have experience with research grants? Are you interested in sustainable community...including grant readiness, funding opportunity research, application writing and development, project ...

... my introduction to protocols.io, I decided to research what other online (and mobile) tools are available...protocols, and courses, as well as a space for research labs to create their own lab or group websites...Protocol Online that you might find useful for your research. LabGuru Website: http://www.labguru.com/ Labguru...inventory, managing orders, tracking students and research projects, electronic lab notebooks and protocol...protocols to the protocols.io app so that all of our researchers can easily take those protocols to the lab bench...Data Freedom: The Expansion of Data Sharing in Research Publications by Jim Woodgett        ...

...array of ready-to-use viral vectors available to researchers around the world. While we still offer many resources...vectors from the Addgene repository can save researchers significant time and removes a variable from ...2023, we shared 93,491 viral vector preps with researchers in 45 different countries — and as of 2024, we...Addgene catalog would be broadly useful to the research community. It does, however, necessitate us first...the Data Hub before selecting an AAV can help a researcher make a more informed choice on which AAV to request...developed through the GENIE project at HHMI’s Janelia Research Campus on the GENIE website.   Bibliography  ...

...If you are job searching, you may be wondering if you need to go to the effort of writing a cover letter...the specific company or lab. Take some time to research the lab or company via their papers and/or website... topic]. I am extremely interested in pursuing research in [specific topic] and in the possibility of ...of joining your team as a postdoctoral researcher. Dear Hiring Manager, I am writing to apply for [title...over [number] years of experience in [field of research], and have excellent [list the most important ...'s mission, or your deep interest in the lab's research. Quick tips for writing a good cover letter Personalize...

... neural circuits is essential for neuroscience research. The use of genetic tools for visualizing and ...the brain. The Allen Institute and the Janelia Research Campus in the USA have used AAV1 and AAV2 serotypes...atlas of the mouse brain. This atlas enables researchers to quickly find the best AAV serotypes to target...tags to monitor single molecules - something researchers often want to do at neuronal synapses for instance...different FPs that emit across the visible spectrum, researchers can also use multiple FPs in single experiments...limitations of existing FP and epitope tags, researchers in Loren Looger’s lab have developed new molecular...

...A recent survey of PhDs found that many researchers feel that they lack formal training in a variety ...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working...aforementioned survey as one of the the skills favored by research-intensive careers. External collaborations are... partnerships are also very important in a non-research context, especially when considering small to ...connection by commenting and sharing. LinkedIn or ResearchGate allow you to send personal updates as messages... your organization is important for success in research-intensive careers. In addition, this skill is ...

... emotional phenomena like stress levels, but researchers have to be mindful of motivation overlap. The... involved in locomotor sensitization? Previous research has shown that regions such as the nucleus accumbens...thought to be involved in these diseases. The researchers injected Cre-expressing AAV-5 into the DRN of...supports the relevance of BDNF in depression. The researchers found that injecting lentiviral vectors expressing...than those that were not (Hare et al., 2019). The research described here is only a small selection of the... viral protocols and videos Find AAVs for your research ...

...contributed by Katherine Rogers, a postdoctoral researcher at the Friedrich Miescher Lab of the Max Planck...or morpholino. This observation unlocked novel research into genetic compensation, a previously unknown...neurons can be fluorescently labeled, allowing researchers to observe changes to these tissues under different... the embryo—have proven useful in a variety of research applications. Lineage tracing studies highlight...Society. Katherine W. Rogers is a postdoctoral researcher in Patrick Müller’s group at the FriedrichMiescher...molecular biology reference Find plasmids for your research ...

... Resources on Addgene.org Search for AAV vectors to use with your research. Visit Our AAV Guide Page ...at the University of Pennsylvania independently searched for this unique sequence of DNA and both identified....B. The Deverman Lab took a broad approach and searched for genetic difference between the 36 mouse strains...of the Mouse Genome Project. They focused their search on SNPs and small insertions and deletions (indels... sequencing of related hybrid mice narrowed the search to a ~4.5 Mbp region of the mouse chromosome. Of...

...formative experience nailed down my plan to become a research scientist. I recently was invited to attend the... need to be developed for each organism, but researchers are optimistic that this can be done for most....” Accepted proposals were required to present research plans that went beyond obtaining “omic” data and...data, and tools that result from their work. Researchers are encouraged to not just wait for papers to...melanogaster or Saccharomyces cerevisiae (both fine research organisms) -  perhaps you’ll choose to study Crepidula... Milkweed plants are known to many as the only Monarch butterfly food. They have chemical pathways generating...

... in Biomedical Research from Albert Einstein College of Medicine. Her doctoral research was conducted ...Jack Griffith, her research focuses on the replication machinery of Kaposi’s sarcoma-associated herpesvirus...Figure 1). When behaving properly, FPs enable researchers to investigate the localization and dynamics ...secretory pathway."Biochemical and biophysical research communications 430.3 (2013): 1114-1119. Pubmed...

...equal distribution. Lab tip #3: Barcode everything Use barcodes and scanners to keep track of reagents...range from technical tips as in our episode on Barcoding, to basic lab etiquette that will keep the lab...reagents in your lab. With barcode tracking, you will always be able to locate materials quickly and easily...easily. Learn more about using barcodes. Check out our “Lab Tip” filter on the blog to keep up to date...

...exist for a variety of species, cell types, and research applications. Labeling neurons with Brainbow The... used alongside common, existing tools for fly research that use Flp recombination. In addition to the...small population size of CFP++ cells permitted researchers to longitudinally map the turnover of Langerhans...such as the brain, transparent so that the 3D architecture of a fluorescent neuron is visible. Chemical...more details, check out this blog post from the researchers who developed Tetbow. Conclusion Stochastic multicolor...science 59.3 (2018): 1630-1640. Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright multicolor ...

...If you’re using antibodies in your research, you've probably found yourself staring at a browser full... this decision. In the open-access Data Hub, researchers interested in the Addgene antibody collection...Addgene Data Hub.   Antibody data reports can be searched and sorted by gene/target, name, application, ...the results include knockout data.    Figure 2: Search options in the Antibody Data Hub. Data reports  ...data, including negative data, which can help researchers save time and money when selecting an antibody...

...You can also use the "Search Table" box at the upper right of the table to search and filter the list of...bulleted list of plasmids is associated with the "Barcoding cells using cell-surface programmable DNA-binding...here to explore the TetO-FUW-OKSM plasmid page. Or search for your favorite plasmids and see where they've...the article that has used this plasmid. Happy researching! If you need any assistance finding plasmids ...

... to our audience. How can researchers work with you? We welcome researchers from all fields of science...utilize tax-payer money and government funding for research, and also to encourage women, students, and underrepresented...convey without having to extensively use Google search. Be careful about hidden connotations behind a ...ambassador, SfN community leader, and an early research career panelist at BioTechniques.     Additional...