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We narrowed to 651 results for: emb

Showing: 361 - 380 of 651 results
  1. An Introduction to Adenovirus

    Type
    Blog Post
    ... derived from the mastadenovirus members (mammalian-infecting members) of the Adenoviridae family, which...
  2. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post
    ...!). In 1997, Joseph Provost, then a new faculty member at Minnesota State (and now Professor of Chemistry...and it can be difficult to give up the control embedded in “cookbook” lab activities (follow the recipe...
  3. Tips for arabidopsis transformation

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    Blog Post
    ...2007). Alternatively, if you prefer Golden Gate assembly, there are a series of plasmids from the Dinneny...Arabidopsis.html?id=IfZAMNPWVk4C&redir_esc=y (Accessed: 21 September 2018). Additional resources on the Addgene blog...
  4. Plasmids 101: Colony PCR

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    Blog Post
    ...want to know the orientation of the insert. One member of this type of primer pair anneals to a sequence...Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org Read about...
  5. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post
    ...nucleus. To circumvent this requirement for transmembrane proteins, a split ubiquitin system has been ...genetic system optimized for systematic studies of membrane protein interactions. Proc Natl Acad Sci U S A...
  6. Your Lentiviral Plasmid FAQs Answered

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    Blog Post
    ...technically gamma-retroviruses - another, separate member of the retroviral family. While both lentiviruses...Lentiviruses are able to enter an intact nuclear membrane allowing them to infect both dividing and non-...
  7. Negotiating Work and Life: How to Find the Joy

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    Blog Post
    ...Maybe you’ll need to care for an elderly family member, want to train for a triathlon, finally buy that...laundry, take public transportation, call a cab and remember their own school assignments, the better. In my...
  8. CRISPR 101: Any Base Transversion Editors

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    Blog Post
    ... thereafter, using mouse instead of human AAG (remember, MPG and AAG are the same protein!) (Chen et al...A•T-to-C•G base editing in mammalian cells and embryos. Nature Biotechnology, 42(4), 638–650. https://...
  9. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...interested in working for this lab and/or company. Remember to proofread your cover letter, and if possible... originally written by Maria Soriano-Carot in September 2016 and was updated by Rachel Leeson in May 2024...
  10. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    Blog Post
    ...cleaving genomic DNA in mammalian cells and zebrafish embryos, and they can be used to modify genomic loci that...SpCas9 (Amrani et al., 2018). When choosing a Cas9, remember to check that your tracrRNA and crRNA (or synthetic...
  11. Hot Plasmids - August 2020

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    Blog Post
    ...with plasmids encoding FPs tagged with the plasma membrane targetin sequence derived either from p63(1-29...non-specific labeling. For labeling the plasma membrane, they tested different target sequences for improved...
  12. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post
    ...Members of the bacterial world produce an assortment toxins to claim territory or kill competing microorganisms... (hok), a long-living toxin that damages cell membranes; Suppression of killing (sok), a short-lived RNA...
  13. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...for Excellence in Molecular Plant Sciences since December 2020. His research focuses on the efficiency of...for Excellence in Molecular Plant Sciences in September 2019 as a Ph.D. candidate. Her research focuses...
  14. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...https://youtu.be/EHHhiawIdWM Saving salmon, one embryo at a time – A video by LeeAnne Thayer, a Ph.D. ...University of Maine, about her research on plummeting embryo survival rates. Video on We Share Science: http...
  15. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post
    ...harboring this mutation are deficient in outer membrane protease VII, which reduces proteolysis of the...pLemo (CamR) Expression of toxic, insoluble, or membrane proteins  T7 Express   IPTG-inducible expression...
  16. Plan Your Experiment

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    Collection
    ...read our three tips to improve HDR efficiency . Remember that HDR also requires donor DNA to serve as template...can transfect at high efficiency, such as human embryonic kidney 293 (HEK293) cells. For more difficult ...
Showing: 361 - 380 of 651 results