We narrowed to 437 results for: ena

1. AAVs CREATed for Gene Delivery to the CNS and PNS

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   Blog Post

   ...making pretty pictures (see figure 4). It also enables single-cell anatomical study and neuronal tracing...

2. Droplet Digital PCR for AAV Quantitation

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   ...Increasing the elongation time to 2 minutes and the denaturation time to 1 minute has been shown to increase ...

3. Simplify Cloning with in vivo Assembly

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   ...beta-actin promoter of the pHLsec vector. In this scenario, IVA can be employed by cutting the vector with...

4. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   Blog Post

   ...of green fluorescent protein (GFP) sparked a renaissance in biological imaging. Suddenly, cell biologists...

5. Tips for Screening with Yeast Two Hybrid Systems

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   ...two halves of the luciferase protein interact and enable intein splicing (5). Screening with yeast two hybrid...

6. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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   ...-toxic and could be expressed in living cells, enabling the study of dynamic, physiological processes....

7. Negotiating Work and Life: How to Find the Joy

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   ...home no matter how busy I am. Believe it or not, teenagers just go to bed and you’ll never know it so don...

8. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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   ...PMC5424143. 2. Tsai, Shengdar Q., et al. "GUIDE-seq enables genome-wide profiling of off-target cleavage by...

9. Analyzing CRISPR Editing Results with ICE from Synthego

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   Blog Post

   ...quantitative, NGS-quality analysis of CRISPR editing - enabling a ~100-fold reduction in cost relative to NGS-...

10. Deciphering the Mysteries of Behavior with Viral Vectors

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    Blog Post

    ...be used as a readout for associated emotional phenomena like stress levels, but researchers have to be...

11. Save Time with Transient Plant Leaf Transformations

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    Blog Post

    ...cultures or plants, our transient expression method enables us to quickly screen and investigate transcription...

12. CRISPR-mediated Plant Base Editors

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    ...gene system to clone multiple protospacers which enables us to target more than one genomic site simultaneously...

13. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ...restriction-modification systems mediate plasmid maintenance." Journal of bacteriology 177.12 (1995): 3451...

14. Finding nucleic acids with SHERLOCK and DETECTR

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    Blog Post

    ...amplification step in combination with the ssRNA reporter enables SHERLOCK to detect DNA or RNA with atomolar sensitivity...

15. Plasmids 101: Gateway Cloning

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    ... variety of plasmids. Speed The Gateway system enables the generation of the expression construct in only...

16. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post

    ...specific attachment sites via sequence engineering, enabling even more efficient integration activity. They...

17. Viral Vectors 101: Pseudotyping

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    Blog Post

    ...glycoprotein pseudotyping of lentiviral vectors enables retrograde axonal transport and access to the nervous...

18. Twenty Years of Sharing: Expanding Accessibility

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    Blog Post

    ...classroom, in their lab, in their experiments, to enable virtual learning during the pandemic. Feedback ...

19. Twenty Years of Sharing: Addgene's Viral Vector Service

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    ...using/depositing. Our viral vectors data is less amenable to such analysis, due to its inherent bias: Addgene...

20. Hot Plasmids Spring 2024

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    Blog Post

    ...PE7, it will be exciting to see future studies enabled by this tool as well as additional potential improvements...