We narrowed to 636 results for: erf

1. Deep Dive: qPCR

   Type

   Blog Post

   ...most machines have a protocol that automatically performs the normalization step.  Machines Most general-use...the results are correct. Primers and probes can interfere with amplification reactions, and reagents can...

2. Technical Design of a Western Blot

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   Blog Post

   ...different than the validation assays, we recommend performing your own validation assays. If you’re using previously...interest is not available in a pure form, you can perform relative quantification, which measures protein...

3. Protocol - pLKO.1 – TRC Cloning Vector

   Type

   Protocol

   ...antibiotics (no penicillin or streptomycin). Day 2: b. Perform the transfection in the late afternoon because ...

4. Suggest A Plasmid You'd Like to Find at Addgene

   Type

   Blog Post

   ...thought to yourself, “Oh! This plasmid would be perfect for my next experiment!” but were unable to find...

5. The 12 Days of CRISPR: 2021

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   Blog Post

   ... day of CRISPR, Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth...

6. The 12 Days of CRISPR

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   Blog Post

   ...CRISPR, Addgene gave to me: CRISPR activation and interference screens for primary human T cells🎵 On the 3rd...

7. Top Requested Plasmid of 2017 - pMD2.G

   Type

   Blog Post

   ...VSV-G envelope protein under the control of the powerful mammalian CMV promoter. Envelope proteins are ...

8. New Educational Resource: The Addgene Videos Page

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   Blog Post

   ...our brand new Addgene Videos page! Videos are a powerful tool for communicating scientific knowledge. On...

9. Celebrating the 2024 Nobel Prize in Chemistry

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   Blog Post

   ...Kuhlman and Dantas et al., 2003). That made it a powerful proof of concept that this kind of protein design...

10. Twelve Amazing Guest Blog Contributors!

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    Blog Post

    ...deposits from Arlene Sharpe’s lab. X-Chime and Performing In Vivo CRISPR Screens Using the FITS Approach...

11. Addgene Protocols Go Viral: AAV Purification and Titration

    Type

    Blog Post

    ...unloading of the column and give some tips for performing this purification.  Check out the AAV Purification...

12. Deck the Lab 2022!

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    Blog Post

    ...Falcon tube trees to giant paper mache Krampus performing routine lab maintenance tasks. Hot tip: make ...

13. PEI Calculator for Planning AAV Packaging Transfections

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    Blog Post

    ... what type of tissue culture dish you will be performing the transfection in: 15 cm dishes OR CF5 stacks...

14. Streaking for Single Colonies: The Streak Plate Challenge

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    Blog Post

    ... available from Addgene, he knew it would be a perfect fit for the streak plate challenge. Chromoproteins...

15. Editor's Choice, October 2016

    Type

    Blog Post

    ...had our yearly Halloween party (see all of our wonderful costumes in the pictures below, yours truly is...

16. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type

    Blog Post

    ... biology students about the functions of these powerful tools and to use them as platforms for the construction...

17. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

    Type

    Blog Post

    ...characteristic allows monoclonal antibodies to be used as a powerful reagent in the laboratory.   The DSHB focuses ...

18. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

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    Blog Post

    ...our system,” Sakuma said. “These are sure to be powerful tools to modify the expression of multiple genes...

19. What's New in CRISPR - Winter 2018

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    Blog Post

    ...plants, it has been difficult to compare the performance of different nucleases. Nicola Patron’s lab compared...

20. Inntags: Innovative Protein Epitope Tagging

    Type

    Blog Post

    ...antibody. There is one major caveat - a tag may interfere with protein localization and/or function, so ...