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  1. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...technique utilizes Cas9 proteins harboring receptor ligands (C in the figure above), which result in the internalization...allowing for ligation to pyridyl disulfide-activated ligands. Due to the concerns of off-target mutations and...

  2. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...these vectors for gene therapy. In 2003, VIRxSYS began the first clinical trial with a lentiviral vector... vector. Naldini L, Blömer U, Gallay P, Ory D, Mulligan R, Gage FH, Verma IM, Trono D. Science. 1996 Apr...

  3. CRISPR Antimicrobials

    Type
    Blog Post
    ...their suppliers, and public health and medical organizations are working to help educate the public about...chance of an opportunistic infection like for organisms like C. difficile. In 2014, Citorik et al. and...

  4. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...directly from A. Victoria and expressed in any organism while still maintaining fluorescence. The protein...soon as its sequence was elucidated, scientists began engineering new versions of GFP through mutagenesis...

  5. Hot Plasmids - November 2023

    Type
    Blog Post
    ...proximity labeling experiments in cells, tissues, and organisms. Lee, S.Y., Cheah, J.S., et al. (2023). Engineered...hottest antibodies in Addgene’s collection since we began distribution and has been verified to perform well...

  6. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...specific to that country. ‌For example, when we began offering temperature-sensitive materials, we noticed...materials and learn about them. As early as 2007, we began grouping similar tools together in what would eventually...

  7. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ... salaries (which have been made by multiple organizations across academia). Increased postdoc wages, overtime... salaries as their website implies. These 5 organizations, according to the NSF data, account for 18% ...

  8. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...and stably maintain the genes they carry among organisms belonging to many different phylogenetic groups...bacteria and express proteins within different host organisms. pBBR1-based plasmids and plasmids in incompatibility...

  9. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...region (approximately 500bp is best) and select the organism. It will then tell you the available gRNA targets... much more from our favourite mammalian model organisms in the near future.  Many thanks to our guest...

  10. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...plant tissue cultures.   C. roseus is not a model organism such as Arabidopsis or tobacco. Studying gene ...transgenic plants is a common problem in non-model organisms. Therefore, transient methods are usually the ...

  11. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...-coupled receptor (GPCR) recognizing a specific ligand (Ino et al. 2022). The oxytocin sensor MTRIAOT ...various neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP to urotensin. The MTRIA...

  12. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA synthetase, despite... Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156...

  13. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...functional, fluorescent protein in almost all organisms and cell types, (ii) no need to add a co-factor...mTurquoise2, not shown) remains fluorescent in acidic organelles (Figure 1), showing that its acid tolerance is...

  14. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based technique, validation...you are confident, especially if you work in an organism that is underrepresented in the biomedical research...

  15. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...difficult given that the viral reservoir spans multiple organ systems. Both approaches may prove more successful...Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young WB, Khalili K, Hu W. CRISPR...

  17. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...which allow AAVs to broadly spread throughout an organism, including difficult-to-target tissues like the...Byrne, L. C., Klimczak, R. R., Visel, M., Yin, L., Merigan, W. H., Flannery, J. G., & Schaffer, D. V. (2013...

  18. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...membrane on an agar plate selective for your target organism and incubate overnight according to appropriate...on hand that will specifically bind your target organism, it can be helpful to spot this protein directly...

  19. Finding Your Perfect Job After University

    Type
    Blog Post
    ...Luckily, I have worked for a variety of different organizations, so I tend to provide them with an overview ...practices, but can sometimes go unnoticed at large organizations. Another downside to large commercial companies...
Showing: 361 - 380 of 607 results