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Plasmids 101: 5 factors to help you choose the right cloning method

Type

Blog Post

...technologies currently available, you now face the challenge of trying to figure out what system might work...
Transferable Skills Guide: Managing a Team

Type

Blog Post

.... Personally I found this to be particularly challenging. As PhD students and postdocs we are used to ...
Truncated gRNAs for Regulating Gene Expression

Type

Blog Post

...different perturbations. Consider, for instance, the challenge of attempting to simultaneously and selectively...
Plasmids 101: Optimizing Plasmid Yields

Type

Blog Post

...and can therefore be cultured in media containing half the normally recommended antibiotic concentration...
PITChing MMEJ as an Alternative Route for Gene Editing

Type

Blog Post

...cell lines can be created in about a month and a half, without the need for complicated cloning of homology...
Plasmids 101: Multicistronic Vectors

Type

Blog Post

...segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes...
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Type

Blog Post

...fluorescent proteins. Visualizing fluorescence can be challenging in plants because of the auto-fluorescence of...
Great Results Start with Great Standard Curves

Type

Blog Post

...allergy testing lab, and the grad student down the hall from you have in common? All of them rely on standard...
An Introduction to Adenovirus

Type

Blog Post

... like other viral vectors, though significant challenges remain (Wold & Toth, 2013). While the pre-existing...
Summer SciComm Series: Modes of Communication

Type

Blog Post

...graphics for visuals. However, it’s much more challenging to design a hands-on interactive activity using...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

...also theoretically possible, but likely more challenging, with lentiviral expression. For more detail ...
A Guide to Starting Your Own Journal Club

Type

Blog Post

...success” of the journal club. This can be a challengingly vague metric but there are a few things you ...
SciComm: Taking Science to Elementary and Middle Schools

Type

Blog Post

...much in the making. One of the exciting, and challenging, aspects of talking to 8th graders was the broad...
Tips for arabidopsis transformation

Type

Blog Post

... method of identifying transformed Arabidopsis thaliana seedlings following floral dip transformation’...
Simplify Cloning with in vivo Assembly

Type

Blog Post

... to amplify away from the insertion site. Encode half the new sequence at the 5’ end of each primer, with...
AAVs in Retinal Gene Therapy

Type

Blog Post

... completing her PhD and medical training in Ophthalmology. Gene therapy was in its infancy, but the eye...
Transferable Skills Guide: Identifying Your Transferable Skills

Type

Blog Post

...presents itself even outside of work, consider challenging yourself, take the opportunity, and discover ...
Better Dyeing Through Chemistry & Small Molecule Fluorophores

Type

Blog Post

... include: Self-labeling tags (e.g., SNAP-tag, HaloTag, TMP-tag) – These widely used systems consist of...
Tips for Screening with Yeast Two Hybrid Systems

Type

Blog Post

...functional luciferase only when fusions of the two halves of the luciferase protein interact and enable intein...
A Career in Grants: How To Become a Grant Professional

Type

Blog Post

...Becoming a grant professional is rewarding and challenging! Jo Miller, GPC, CSMS is the founder and principal...

Showing: 361 - 380 of 430 results

Results per page:

Plasmids 101: 5 factors to help you choose the right cloning method

Transferable Skills Guide: Managing a Team

Truncated gRNAs for Regulating Gene Expression

Plasmids 101: Optimizing Plasmid Yields

PITChing MMEJ as an Alternative Route for Gene Editing

Plasmids 101: Multicistronic Vectors

Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Great Results Start with Great Standard Curves

An Introduction to Adenovirus

Summer SciComm Series: Modes of Communication

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

A Guide to Starting Your Own Journal Club

SciComm: Taking Science to Elementary and Middle Schools

Tips for arabidopsis transformation

Simplify Cloning with in vivo Assembly

AAVs in Retinal Gene Therapy

Transferable Skills Guide: Identifying Your Transferable Skills

Better Dyeing Through Chemistry & Small Molecule Fluorophores

Tips for Screening with Yeast Two Hybrid Systems

A Career in Grants: How To Become a Grant Professional

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