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We narrowed to 850 results for: met

Showing: 361 - 380 of 850 results

  1. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ..., starvation or the use of caloric restriction mimetics trigger autophagy and, in a mouse model led to...anti-cancer immunosurveillance and reduced tumor mass. Methods to study autophagy can be found here. Fun fact:...immunotherapy is the combination of the CRISPR/Cas9 methodology with T cell receptor engineering (Ref 5)  as ...Pietrocola, Federico, et al. "Caloric restriction mimetics enhance anticancer immunosurveillance." Cancer...

  2. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ... remind you of anything? Perhaps the Scientific Method? Imagine your problem is just a question you have... skills” is the ability to apply the scientific method to problems in a variety of contexts.  How do you...skills? Thankfully, as scientists, the scientific method is a core part of our academic training.  Developing...their logic. Or you can try applying the scientific method to everyday problems in your life. This tactic ...

  3. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...explained in more detail here. There are a variety of methods that can be used to measure your fluorescent protein...complicating the interpretation. Because of this, these methods only give a qualitative view of dimerization tendency...the protein forms dimers. Finally, spectroscopic methods (based on fluorescence polarization) can be used...homodimerization. However, all the aforementioned methods only determine the capacity of homodimerization...

  4. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...sophisticated methods of sex and gender analysis. Universities need to incorporate these methods into their...Innovations. This project: 1) develops state-of-the-art methods of sex and gender analysis and 2) provides case...For more practical help, there are numerous methodological articles to consider—we have loaded those we...

  5. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ...is sifting through the long list of titles for something that is actually useful to you. When embarking... the jargon and background in your field, but sometimes papers are also just poorly written. By nature... or are trying to replicate an experiment, the methods section probably won’t get much attention reading...of the appropriate controls? Refer back to the methods section for finer points of the protocols. Are ...

  6. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...repeats separated by an 8-bp long asymmetric core spacer sequence. The asymmetry in the core sequence gives ...specified times. Combining this with some of the loxP methods described above, a genetic modification can be ...site have been created, which contain a unique asymmetric spacer "NNNTANNN", where "N" indicates which ...

  7. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... tool in the field as it provides a noninvasive method for manipulating cell activity, especially neuronal... a chemogenetic system, you’ll need to choose a method for targeting receptor expression as a ligand and...expression must be restricted to specific circuits. Methods for targeting expression involve tissue specific...as a DREADD agonist due to its ability to be metabolized into clozapine (CLZ). While some scientists argue...

  8. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...more than one protein in your sample? Or what if something weird happened during your western and your results...antibodies, especially with more stringent stripping methods. If you are planning to detect multiple proteins... most PVDF membranes recommend re-wetting with methanol before rinsing with wash buffer, as dry PVDF won... that has been dried, no matter what stripping methods you throw at it. Ideally, dry your membrane once...

  9. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...pieces of the FP back together (Figure 1). This method is generally known as Bimolecular Fluorescence ...GFP reassembly is a fast, efficient, and robust method for detecting protein-protein interactions in vivo...options, check out the 2in1 Kit for FRET and ratiometric BiFC from Christopher Grefen’s lab or explore...Remember that the FP fragments themselves can sometimes interact in cells and produce background signal...

  10. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ...beginning. Variability source Standardization parameters Animal Strain, age, sex, housing conditions...reduce expression levels of certain genes. A popular method uses Cre recombinase on transgenic flox animals... area of interest may be very limited with this method, especially in adult animals, and not targeted ...order to maximize possible effects. Injection Parameters: The injection needle should be as thin as possible...

  11. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...the laser and measuring its output with a light meter before and after polishing. Efficiency of the implant...series already provided or check the materials and methods of a paper that uses the variant you wish to use... pulses. An example of a standard stimulation parameter is 20Hz (laser output), 5ms “on”, 50ms “off”.  ...lasers are also available. Optimal stimulation parameters must also be determined empirically but it’s ...

  12. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...screens can be carried out using a few different methods — in vivo, in vitro, and ex vivo. The general process..., so having an efficient and effective delivery method is vital for the best outcomes. The go-to in vivo...vivo option AAV has become the preferred delivery method for in vivo CRISPR screens. AAV vectors can be ...that is unmatched by other viral vector delivery methods. Lentivirus has limited packaging options, with...

  13. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...luminescence, which require UV lamps, fluorometers, or luminometers, chromoprotein detection can be done...taken up the plasmid. Of course, other follow up methods like sequencing are more definitive, but visual...before taking on more time consuming and expensive methods. While techniques such as  blue-white screening...

  14. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...yields by stabilizing rate-limiting enzymes. In this method, the major capsid protein VP1 is co-expressed with...Cheah et al., 2021.   After validating this method through monitoring GFP degradation, this protein...acid, suggesting that MPyV system can improve metabolic pathway yields by encapsulating a single enzyme...pathway, the MPyV system provides a simple, efficient method for protecting a single enzyme that drives biosynthetic...

  15. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...(Anzalone et al., 2019; Chen & Liu, 2023). This method uses a prime editor (typically a Cas9 H840A nickase...pegRNAs in one experiment (Figure 1). Through this method, we found key determinants of prime editing efficiency...Koeppel et al., 2023), their predictive power is sometimes limited across different targets due to variations...Parallel; Akhtar et al., 2013; Schep et al., 2021) method to link prime editing efficiency to chromatin data...

  16. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...engineered nucleases. Nat Methods 11:1051–1057 . https://doi.org/10.1038/nmeth.3075 Lukashev AN, Zamyatnin...Scientific Reports paper introducing the delivery method in 2014, Manuel Goncalves lab report that AdV-mediated...lab found in a second paper published in Nature Methods the same year that delivering RNA-guided nucleases...

  17. Transferable Skills: Negotiation

    Type
    Blog Post
    ...grad student, I thought that “negotiation” was something that I would mainly do over the price of a used...unprepared. Mainly, I thought of negotiation as something done not by people wearing a lab coat, but instead... argument but felt bad afterwards). Or worse, sometimes there’s no agreement at all and everyone leaves...example provides a scenario where both people get something valuable. Importantly, as the third example shows...

  18. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...company is “…where molecules become real”.  We sometimes forget that the models we create are around 30...interrogation process, they will almost invariably say something like “Oh,…I didn’t quite appreciate that….” For...determined electron density, have been known to say something silly like “See there, arginine 53 really is right...them to slow down and focus their attention on something tangible. Under the guidance of a trained educator...

  19. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...mother. Inside the pupal case, the larva will metamorphose into its adult form. In all, it takes roughly...homozygous alleles, others cannot. Geneticists need a method for maintaining stable lines of genetic mutants...recombine chromosomes during meiosis. This can sometimes be a handy feature when setting up genetic crosses...symbol”. Additional Resources on the Addgene Blog Methods for Site Specific Recombination in Drosophila Learn...

  20. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ... genome engineering parameters via a library-on-library approach." Nature methods 12.9 (2015): 823-826...RNA-guided gene regulation and editing." Nature methods 10.11 (2013): 1116-1121. PubMed PMID: 24076762....genome editing, activation and repression." Nature methods 12.11 (2015): 1051-1054. PubMed PMID: 26344044....
Showing: 361 - 380 of 850 results