We narrowed to 416 results for: nans

1. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   ...  More resources on Addgene.org Ready-to-use recombinant antibodies at Addgene Addgene's Antibody Guide...

2. Five Popular Model Organisms

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   Blog Post

   ...gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming sequences." The...

3. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

   Type

   Blog Post

   ...fragments are likely non-functional, they could be dominant-negative and/or toxic. A potential solution is...

4. CRISPR 101: Homology Directed Repair

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   Blog Post

   ...The size of the intended edit is the biggest determinant when selecting a type of donor. ssDNA templates...

5. Antibodies 101: Introduction to Immunofluorescence

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   Blog Post

   ...Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...

6. Plasmids 101: Expressing shRNAs for RNA Interference

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   Blog Post

   ...-3_10 Vergani-Junior, C. A., Tonon-Da-Silva, G., Inan, M. D., & Mori, M. A. (2021). DICER: structure, ...

7. Multiple Plasmids at a Low Price: Kits from Addgene

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   Blog Post

   ...Protein Expression Kit has been used to produced recombinant proteins for in-vitro assays: Koch, Daniel, ...

8. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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   Blog Post

   .... Cell Rep. 2016 Apr 5. PubMed PMID: 27068471. Ramanan V, Shlomai A, Cox DB, Schwartz RE, Michailidis ...

9. Pouring LB Agar Plates

   Type

   Protocol

   ...recommended temperature should kill most potential contaminants, certain types of spores will stubbornly survive...

10. Western Blot

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    Protocol

    ... Antibody Guide Immunocytochemistry Protocol Recombinant Antibody Purification Protocol Introduction Western...

11. Pouring LB Agar Plates

    Type

    Protocol

    ...recommended temperature should kill most potential contaminants, certain types of spores will stubbornly survive...

12. Western Blot

    Type

    Protocol

    ... Antibody Guide Immunocytochemistry Protocol Recombinant Antibody Purification Protocol Introduction Western...

13. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    ...administered systemically, without causing disease? Recombinant adeno-associated viral vectors, or rAAVs, can...

14. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post

    ...https://doi.org/10.1093/nar/gkw159 Williams RM, Senanayake U, Artibani M, Taylor G, Wells D, Ahmed AA, Sauka-Spengler...

15. CRISPR 101: Off-Target Effects

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    Blog Post

    ...CRISPR work, this often means DNA cleavage at unanticipated sites. While off-target effects are nearly impossible...

16. PiggyBac-ing Through the Genome Editing Field

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    Blog Post

    ...or when the gene is constitutively-active or a dominant-negative mutant. Reducing the copy number can ...

17. Finding Your Perfect Job After University

    Type

    Blog Post

    ...American system), in 2009 (at the beginning of the financial recession) I knew obtaining work in scientific...

18. AAV ddPCR Titration

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    Protocol

    ...droplet digital PCR (ddPCR) to titer purified recombinant Adeno-associated viral vectors (AAV). This protocol...

19. AAV ddPCR Titration

    Type

    Protocol

    ...droplet digital PCR (ddPCR) to titer purified recombinant Adeno-associated viral vectors (AAV). This protocol...

20. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type

    Blog Post

    ...cells are growing so slowly, there isn’t much maintenance involved. Also it was really fun to find individual...