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  1. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...intact β-galactosidase produces pigment from x-gal (included in the transformation plate medium), turning the... colony blue. We should note that IPTG is also included in the medium to ensure transcription of the lac...

  2. Addgene: The First Twenty Years

    Type
    Blog Post
    ...we’ll be expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene. I think...growing library of antibody educational resources, including our new Antibodies 101 eBook, the Antibody Guide...

  3. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...They are often used for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed...molecules can bind to a wide variety of targets, including proteins, peptides, amino acids, drugs, metal ...

  4. Antibodies 101: Selecting the Right Antibody

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    Blog Post
    ...pillars for antibody validation.  The 5 pillars include genetic strategies that test expression in knock-out...conditions. Be prepared to troubleshoot and be sure to include proper positive and negative controls as you get...

  6. Celebrating 15 Years of Scientific Sharing

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    Blog Post
    ...ahead of most companies in creating a welcoming, inclusive workplace for everyone. We had Unbiasing Training...movement. We are carefully attentive to genuine inclusion, but not worried because we are ahead of the curve...

  7. RNA Extraction Without A Kit

    Type
    Blog Post
    ...proteins that are found in and on everything, including your skin. Here are some general tips for working...degrade RNA. Keep a clean work area, which may include spraying your bench down with a product to get ...

  9. To Each HIS Own

    Type
    Blog Post
    ...Bornhorst 2000). To prevent this, wash steps often include low concentrations of imidazole. The concentration... C-terminus and how many histidine residues to include.  Placement As far as placement goes, there is ...

  10. All in a Twist: dsRNA

    Type
    Blog Post
    ...associated with cellular damage and dysfunction, it is unclear what the precise mechanism behind the production...been documented in several autoimmune disorders, including some forms of lupus and rheumatoid arthritis. ...

  11. Summer SciComm: Preprints

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    Blog Post
    ...journal for formal peer review. Some organizations, including some journals, also support open peer review or...community significantly sooner. Plus, authors can include the Addgene ID for their deposited plasmids in ...

  12. dTAG - You're it!

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    Blog Post
    ...distinct advantages over pharmacological inhibitors including rapidly depleting a protein-of-interest, increasing...terminus is favorable for tagging. However, if it is unclear which terminus should be tagged, the dTAG lentiviral...

  13. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...photobleaching rate is not linearly related to power, the conclusions reached at high power may not translate to real...cells at a power used for live-cell imaging. To conclude, the most photostable fluorescent protein can ...

  15. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...your expected full plasmid sequences (examples include Benchling, Serial Cloner, and Snapgene), and save...plasmid from its predecessor. Important features may include cloning junctions (where most sequence assembly...

  16. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...sites around the USA undertaking BEST programs, including local schools in Boston: the Boston University...market. Her many notable contributions in this area include publishing the book ‘How Economics Shapes Science...

  17. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...for a successful grant application. It typically includes overview information, key dates, funding description...deliver outsider opinions, and reveal items to include for the next application. Although some comments...

  20. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...Standard Curve To do that calculation, you’ll need to include a standard curve in your experiment. These are ...are wells on the same ELISA plate that include known concentrations of your protein of interest. The optical...
Showing: 361 - 380 of 758 results