We narrowed to 457 results for: nsf

1. Illuminating Epigenetics with A FRET Based Biosensor

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   Blog Post

   ...restriction enzyme digestion, DNA adenine methyltransferase identification, and bisulfite sequencing. ...

2. Gaining Leadership Skills Volunteering at a Professional Organization

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   Blog Post

   ...to think about their career options, gaining transferable skills such as leadership skills can only assist...

3. Plasmids 101: 5 factors to help you choose the right cloning method

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   ...your insert, DpnI digest away the templates, and transform the PCR products into E. coli. Ligases within ...

4. 10 Basic tips for mammalian cell culture

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   ...too young and should not be used for important transfection experiments right after they’ve been thawed....

5. Fluorescent Tagging of Endogenous Genes with SapTrap

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   ...tag. The two PCR reactions are then mixed and transfected along with a Cas9 expression vector. They can...

6. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   ...molecules more closely is Förster Resonance Energy Transfer, FRET. Additional opportunities: Tracking intracellular...

7. Truncated gRNAs for Regulating Gene Expression

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   ...loci within a single cell. One might imagine transfecting cells with both the nuclease-positive and nuclease-null...

8. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   Blog Post

   ... alter the ability of one FP to nonradiatively transfer energy to the second FP, often decreasing the ...

9. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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   ...restriction digest of plasmid DNA, and bacterial transformation. And more plasmid-related educational resources...

10. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post

    ...explains it, her work has always been focused on gene transfer, from transgenes to plants, chloroplast to the...

11. Negative Can Be Positive: Open AAV Data with Addgene

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    ...S., & Klein, R. L. (2018). More expansive gene transfer to the rat CNS: AAV PHP.EB vector dose–response...

12. DNA Purification Without a Kit

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    Blog Post

    ...could interfere with interpreting the results of transfection assays. Go forth and perform your DNA extractions...

13. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    Blog Post

    ... lipoic acid ligase, biotin ligase, phosphopantetheinyltransferase) – These enzymes catalyze the covalent...

14. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    ...enhancers and genome math come in. They help us to transform raw genomic coordinates into meaningful experiments...

15. A Career in Grants: How To Become a Grant Professional

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    ...management, and program evaluation are highly transferable. Use your experience and your passion(s) to ...

16. Plasmids 101: Protein tags

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    ...peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be fused to recombinant proteins...

17. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    Blog Post

    ...of new technologies may be very effective in transforming conventional research. So, I hope my younger...

18. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    ...earliest uses of GFP. Fluorescence resonance energy transfer (FRET): This is used to study the interactions...

19. Plasmids 101: Codon usage bias

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    ...Charles G. Kurland. "Growth rate dependence of transfer RNA abundance in Escherichia coli." The EMBO journal...

20. To Codon Optimize or Not: That is the Question

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    ...important? Translation and protein synthesis relies on transfer RNAs (tRNAs). tRNAs bind to and deliver amino ...