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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 780 results for: scr

Showing: 361 - 380 of 780 results

  1. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...molecular biology experiments was in the job description, but this skill is not just about reacting to...research non-academic careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined...

  3. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...video_player "embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button...', style='' %} Resources on the Addgene blog Subscribe to the Addgene blog Read our viral vector blog...

  4. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...Another similarity to Cas12a is that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged... the genome. Cas13, similar to Cas14, also indiscriminately cleaves ssRNA when engaged at its RNA target...

  5. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...figure out how to optimize protocols for in vivo screening with lentiviral pooled shRNA libraries. Later,... Blog Learn about the Sabatini and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource...

  6. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ... fragments, these problems can be overcome by screening a higher number of potential clones. Golden Gate...In early 2011, the Bogdanove and Voytas groups described a new Golden Gate-based technology for genome ...

  7. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...Cre-dependent gene expression – placing a series of transcriptional and translational termination features with... Combining this with some of the loxP methods described above, a genetic modification can be restricted...

  8. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...method seems simple, I found it hard to remember to describe all four parts of a STAR answer once the interview...hard during this talk since you’ll be sharing your screen and won’t be able to see who’s asking a question...

  10. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...of citations per year and the number of other manuscripts that heavily cite the paper are displayed. You...the context of these citations. The number of manuscripts that cite the background, methods, or results...

  12. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...cloning, the Haynes lab’s Protocols.io protocol describes an elegant system that allows for the assembly...editing assemblies are sensor-actuators, either transcriptional regulators or chromatin-modifying enzymes. ...

  13. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...Resonance Energy Transfer (FRET) was originally described by Theodor Förster in 1948 as a variation of the...FRET probe, try searching PubMed for articles describing the FRET tool that you are looking for and check...

  14. Hot Plasmids February 2024

    Type
    Blog Post
    ...authors used a multispecies approach to generate and screen a library of AAV9 variants (Chuapoco et al., 2023... Figure 3: Overview of library generation and screening that led to identification and characterization...

  15. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...forward as quickly as possible and submitted our manuscript to the journal Cell just six weeks later. The...Asian-, African- and American-lineages). Strain discrimination is especially important in the current outbreak...

  17. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...used to quantitate RNA levels using reverse transcription-qPCR (RT-qPCR). The first step of this process...requires RNA to be converted to cDNA using reverse transcription and the cDNA is subsequently quantified by qPCR...

  18. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...processes. To create the antibody toolbox, they screened antibody candidates using a synthetic human scFV...cross-reactivity, antibody candidates underwent affinity screening to select for slow off-rate binders. The best ...

  19. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...sense, models can be both useful, and dangerous. Subscribe to CRISPR Blog Posts How do educators and students...reaction to this model on his blog. Or, for a description of how effective this model was in explaining...

  20. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...while seated or with your poster on your computer screen than it is to pretend you’re standing at the session...poster board well ahead of time ensures you aren’t scrambling to tack up your poster seconds before the session...
Showing: 361 - 380 of 780 results