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We narrowed to 800 results for: tec

Showing: 361 - 380 of 800 results

  1. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ... IMP USA-Europe not only provide the specific technical and logistic support to increase the chances of...of success, but also nurture a supportive and protective environment where individuals are empowered to...represented a diverse group of individuals with technical and career path needs, and came from the universities...Harvard University, Massachusetts Institute of Technology, Boston University, Johns Hopkins University,...

  2. Important Considerations When Using AAVs

    Type
    Blog Post
    ...Table 1: AAV Serotypes and Tropism Experiment  Detection Method CNS AAV1, AAV2, AAV4, AAV5, AAV8, AAV9...purposes. The University of Pennsylvania and the biotechnology firm Biogen have recently collaborated to develop...is their ability to be used with new exciting technologies (such as CRISPR/Cas9) as they allow for gene... fourth generation of neuroanatomical tracing techniques: exploiting the offspring of genetic engineering...

  3. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...HaloTag or SNAP-tag construct can help you better detect dim structures or low levels of expression with...performance and use super-resolution or single-molecule techniques.   Figure 2: Two-color super-resolution...proteins with small molecules in vivo. Nature Biotechnology, 21(1), Article 1. https://doi.org/10.1038/nbt765... V. (2008). HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis. ACS Chemical...

  4. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...scientific team came as an undergrad at Worcester Polytechnic Institute. Though some WPI students chose to ... physical kits are made and shipped; Chiara, the tech transfer expert who understands the somewhat complicated...repository, performing quality control, and providing technical customer service. Team members are highly trained...a biostatistician, and that one golden-handed technician who can do a certain difficult assay with her...

  5. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... label it with finesse using tRNA suppression technology (Fig. 1) (Dumas, Lercher, Spicer, & Davis, 2015... from depositing labs that have mastered this technique so it might be worth a try, especially since the...References 1. Degorce, François, et al. "HTRF: a technology tailored for drug discovery–a review of theoretical...Time-Resolved FRET-Based Approach for Antibody Detection–A New Serodiagnostic Concept." PloS one 8.5 (2013...

  6. The Twelve Days of CRISPR

    Type
    Blog Post
    ...Addgene gave to me: SHERLOCK and DETECTR, two CRISPR-based methods to detect nucleic acids.  On the third ... CRISPR, Addgene gave to me: five methods for detecting SARS-CoV-2 using CRISPR.  On the sixth day of ...

  7. 8 Great Depositor Research Stories

    Type
    Blog Post
    ... themselves. All of them have cool new tools, techniques, or information that is now available for other...demethylation. AAV Q&A with Tim Miles: While not technically a research story, I couldn't resist adding in...learning about it (and maybe even picked up a new technique or tool to try in your own work!) If you've got...

  8. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    ...particular microorganism, microbial technique, or microbial technology, sign up to write about it on the...CRISPR guide to find out how to use genome editing techniques in your microbe of choice. For more help with...

  9. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    ...using the streak plate technique. Time for the #BioSci3319 #StreakPlateChallenge part 1. Lab B needs a...Sciences at Missouri University of Science and Technology, has been teaching the microbiology lab every...

  10. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    ...CRISPR continues to grow as the genome editing technique has developed and improved. The CRISPR posts written... for more detailed information about this new technology. Our Plasmids 101 series continues to grab scientists...questions or if you have a topic, researcher, or technology you would like us to feature in the coming year...

  11. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...got to learn about intellectual property from our Tech Transfer team members Tony Cova and Chiara DeNatale...DeNatale and programming with our Chief Architect Pam McA' Nulty (check out our blog post on CRISPR software...presentation on the emerging CRISPR genome editing technology was a rousing success! Many of the students were...

  12. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    ... in skeletal muscle by exercise and endurance, protects the brain from depression. Mice that are genetically...stressors as usual, but somehow their brains were protected. Effects of Activation of the PGC-1a1 Pathway ...levels of kynurenine in the body, the brain is protected from stress-induced biochemical changes that could...

  13. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented an exciting way of listening to microbes...vesicles) as acoustic biomolecule-based dyes. This technology is widely applicable as bacterial expression ... high spatial resolution are benefits of this technique. It is even possible to multiplex ultrasound dyes...

  14. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...blog post, keeping up with the newest CRISPR technologies and their applications can be exhausting. A ...addition to mutations in the spacer itself, will protect the HR template from the Cas9. Again, if it's within...of the Cong et. al (2013) Science paper). If you detect positive HR, isolate single-cell colonies, grow...editing, regulating and targeting genomes. Nature Biotechnology, 32(4), 347–355. https://doi.org/10.1038/nbt...

  15. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ...perturbations – in the case of screens using CRISPR technology, gRNAs to knockout genes – are essential screening...the validity of your biological model and the technical performance of your assay. But, in many discovery-oriented... off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed Central...

  16. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...are also the foundation of multiple important technologies, including FRET and optogenetics. Even though...photoconvert GFP from green to RED! This simple technique has been shown to work in plant, Drosophila and...protonated form of GFP is photoconverted, so this technique may not be appropriate for high-pH cellular compartments...

  17. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    ...career goals, and celebrate accomplishments. These techniques are laid out in Vincent et al., and are easily...a recap of accomplishments, from learning new techniques to finally figuring out a tough experiment, sets... in every lab, but the principles behind this technique are beneficial for researchers at all stages. ...

  18. Science as Art

    Type
    Blog Post
    ...inner beauty of familiar flowers." This new x-ray technique provides scientist with a new method to explore...by Darwin on his voyage. Previous destructive techniques were incompatible with the need to conserve these...Richards’ work is held by The Museum of Jurassic Technology in LA, where it is currently on display.   Many...

  19. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...service. A small subset of AAV plasmids will have technical or legal restrictions that make them ineligible...be reviewed by Addgene for legal approvals and technical compatibility with our production process. This...found in our in-stock preps. If a request has technical considerations that may affect expected titers...

  20. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...environmental conditions, cells must be able to detect extracellular stimuli. One way they do so is through...pathways.  This dissociation can be measured using a technique known as Bioluminescence Resonance Energy Transfer...effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists and ...
Showing: 361 - 380 of 800 results