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We narrowed to 57 results for: Feng Zhang

Showing: 11 - 20 of 57 results

  1. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    ...screening. They and their colleagues including Feng Zhang used the GeCKO library to identify genes essential...titer due to the smaller size of the plasmids. The Zhang lab, on the other hand, uses a 1 plasmid, single...Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. Science. 2013 Dec 12. (PubMed). Genome-Wide Recessive...

  2. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...in December, Konermann and her colleagues in Feng Zhang’s lab describe just such a system, which they ...perfect complement for one conducted earlier by the Zhang lab for GeCKO, a lentiviral CRISPR library enabling...enabling genome-scale, knockout screening). As the Zhang lab's Nature paper reports, “Here we have shown ... Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O. Cell. 2014 Feb 27;156(5):935-49. doi... Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2015 Jan 29;517(7536):583-8. doi: 10.1038...

  3. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...: 237-243. PubMed PMID: 20882. Mei, Yuan, and Feng Zhang. "Molecular tools and approaches for optogenetics... 28772120. PubMed Central PMCID: PMC5830081. Zhang, Feng, et al. "The microbial opsin family of optogenetic...membrane potential in response to visible light (Zhang et al., 2011). What do microbial rhodopsins and ...electrophysiological methods (Deisseroth, 2011; Mei and Zhang, 2012; Towne et al., 2016). Optogenetic neuromodulation...neural activity (Bernstein and Boyden, 2011; Mei and Zhang, 2012). For example, blue light activates channelrhodopsins...

  4. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...loading control. 1 lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described in...libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8):783-4. (Link opens...

  5. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...little room for additional regulatory elements. Feng Zhang's group previously packaged Cas9 and multiple ...a smaller, but equally potent Cas9 protein, the Zhang lab took a different approach. They analyzed over...compare the off-target effects of SpCas9 and SaCas9, Zhang’s group used an approach called BLESS (direct in ... test the efficiency of AAV-SaCas9 in vivo, the Zhang lab created an all-in-one SaCas9 and sgRNA construct... Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR... Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing with CRISPR-Cas13. Science ... Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O (2014) Crystal Structure of Cas9 in ...

  8. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...study author F. Ann Ran, a graduate student in Feng Zhang's lab, in a press release. "In the future, it ...

  9. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR FAQs Zhang Lab's CRISPR Frequently ... author of a seminal CRISPR article from Dr. Feng Zhang's Lab, has assembled a list of FAQs about using...author of one of the seminal articles from Dr. Feng Zhang's Lab ( Science , February 2013 ), has assembled...Frequently Asked Questions You may also like... Zhang Lab CRISPR plasmids General CRISPR Plasmids Dr. Le Cong, ...about using the lab's CRISPR technology. Visit the Zhang Lab page and forum to learn more. CRISPR Design ...Double nickase' is a new system, developed by the Zhang lab, which has comparable efficiency to the optimized...nickase system, including one recently from the Zhang group . Many protocols have been published with ...
Showing: 11 - 20 of 57 results