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We narrowed to 93 results for: aav gfp cre

Showing: 11 - 20 of 93 results

  1. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...with AAV-S5E2-GCaMP6f, now available as a ready-to-use viral prep in AAV1!  New ready-to-use AAV prep ...sender” cells express GFP on the surface and “receiver” cells express an anti-GFP nanobody on the cell ...Synaptophysin-GFP. Synapse targeting GCaMP6s from the Rylan Larsen lab, now available in a ready to use AAV5 viral...to track cell-cell interactions By Jennifer Tsang GFP-based Touching Nexus (G-baToN) is a new tool developed...cell surface. When the cells interact, GFP is transferred to the receiver cell, and the receiver cell turns...other fluorophore/antibody pairs. Because the anti-GFP nanobody cross-reacts with BFP, sender cells expressing...Plasmids for delivering CRISPRi components by a single AAV vector are now available from Tonia Rex's lab.  vfCRISPR...

  2. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...sensor was engineered from a GFP variant and a DA receptor such that GFP fluoresces when the receptor ... Yulong Li’s lab has created the GRABDA sensor based on a similar strategy: a GFP variant bound to a DA... DA receptor, such that the GFP fluoresces upon DA-receptor binding (Sun et al., 2018). These sensors ...behaviors. Find dopamine and other biosensors in our AAV vector inventory. References Darvesh, Altaf S.,...viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources ...service Find viral protocols and videos Find biosensor AAVs for your research ...submicromolar) DA affinity and high-magnitude responses (increases in fluorescence) at low concentrations (e.g., ...

  3. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...makes an AAV transfer plasmid an AAV transfer plasmid. ITR sequences are 145 bases each and AAV plasmids... want to do, but it requires some AAV. You’ve never worked with AAV before, but you aren’t going to let...experiments, making AAV starts with some plasmids. You just need three plasmids to start making AAV:  the packaging... the AAV transfer plasmid and take a look at each of its parts. Figure 1: Parts of an AAV transfer...   Key elements of an AAV plasmid First let’s talk about the key parts of an AAV plasmid. Inverted terminal...packaged into the AAV molecule. What’s outside of the ITRs does not get packaged in the AAV vector. The cloning...ITR loss abolishing AAV packaging. To prevent ITR loss, it’s best to propagate AAV constructs in recombination-deficient...

  4. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...and Share AAV Data with Addgene's New AAV Data HubCelebrate Open Data Day with Addgene’s AAV Data Hub!...with AAV vectors in neuroscience Let’s consider my first challenge when I started working with AAV vectors... answers hundreds of AAV questions every year on a range of issues related to AAV vectors, many from people...was: What AAV vector is best for my specific system?  Sharing data and community concerns AAV vectors ...transfer to the rat CNS: AAV PHP.EB vector dose–response and comparison to AAV PHP.B. Gene Therapy, 25(... to express GFP in a group of young rats. We found that, in this case, AAV1 (23%) and AAV6 (<5%, data ... Fig. 1: Testing of three different AAV serotypes (AAV1, 8, 9) injected into the A2 noradrenergic...

  5. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...Flp-dependent Cre AAV in AAV9 and AAVrg. See our new additions here: Cre vectors pAAV-EF1a-fDIO-Cre (121675...: pGP-AAV-syn-jGCaMP7f-WPRE (104488-AAV8) pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (104492-AAV8) pGP-AAV-syn-jGCaMP7c...mCherry)-EGFP (112677-AAV5) Calcium Sensors pGP-AAV-syn-jGCaMP7b-WPRE (104489-AAVrg) pGP-AAV-syn-FLEX-jGCaMP7b-WPRE...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (104493-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (104495-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE.... Our new AAVs include: EGFP-expressing AAV for serotype testing Calcium sensors and GABA sensors Optogenetics...expresses nuclear EGFP in the presence of Cre. Biosensor AAV (calcium sensors and GABA sensors) Calcium sensors... of Cre-dependent GABA sensors. See all our GABA sensor AAV inventory here. pAAV.hSynap.iGABASnFR (112159...

  6. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...self-complementary AAV (scAAV). However, scAAV vectors have limited size compared to single-strand AAV (ssAAV). To ...Voltron2 voltage sensors AAV vectors for targeting microglia The REVeRT Dual AAV Vector System Neurodegeneration...investigators found that administering these AAVs with a dilute Cre-expressing vector resulted in a sparsely-labeled... incorporated in an AAV vector (Figure 5). Efficiency and specificity of these AAV vectors for microglia...Dual AAV Vector System By Vaibhav Kawde To circumvent the payload limitations of traditional AAVs, the...Ace2N-mNeon-A122D and Voltron2 are available as Cre-dependent AAV1 vectors and are suitable for extended imaging...Representative confocal images showing GFP expression from ssAAV5s with hIBA1 or hIBA1a promoters in mice...

  7. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...elements are inserted into an AAV transfer vector and used to generate AAV particles. Transient or stable...common choice for conducting genome-wide screens using CRISPR. AAV transduction Only compatible with SaCas9...reporter protein such as GFP? All of these outcomes can be most effectively achieved with different CRISPR components...typically used for gRNA. May contain reporter gene (e.g. GFP) to identify and enrich positive cells or a selection...transfer vectors. May contain reporter gene (e.g. GFP) to identify and enrich positive cells. Likely contains...or gRNA. Infects dividing and non-dividing cells. AAV is least toxic method for in vivo viral delivery....hard Viral delivery   Lentivirus Retrovirus AAV Cells Dividing or nondividing Dividing Dividing...

  8. An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

    Type
    Blog Post
    ...al. “Cell type-specific manipulation with GFP-dependent Cre recombinase.” Nat. Neurosci. 18(9) (2015):...about Professor Cepko's recent work on developing GFP-activated proteins, read our recent blog post. Check...Multiple Genes to Cells for Gene Therapy 28:40-35:10 : GFP Activatable Tools Being Developed in the Cepko Lab...on the Addgene Blog Read our Recent Blog Post on GFP Activable Plasmid Tools Being Developed in the Cepko... with Michael Koeris Get Cepko Lab Tips on using AAVs in Your Experiments  Resources on the Addgene Website...

  9. Hot Plasmids - August 2020

    Type
    Blog Post
    ...syn-FLEX-axon-jYCaMP1s. Find these AAVs at Addgene Cre-dependent EYFP AAV in the new serotype PHP.V1 that...optogenetic AAV for expression in forebrain GABA-ergic interneurons. These include mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato...vesicular brain cells. Find this AAV at Addgene New serotypes for the Cre-dependent Flpo recombinase pEF1a-DIO-FLPo-WPRE-hGHpA...pEF1a-DIO-FLPo-WPRE-hGHpA, which is now also available in AAV5 and AAVrg. Find these AAVs at Addgene New controls, biosensor...plasmids at Addgene! CRISPR RNP electroporation and AAV donor infection (CRISPR-READI) enables genome engineering...sensors include syn-jYCaMP1s (slow variant), the Cre-dependent syn-FLEX-jYCaMP1s, and the axon-targeted...hDlx-GiDREADD-dTomato and mDlx-ChR2-mCherry. Find these AAVs at Addgene ...

  10. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...combines the Cre/lox system with optogenetics. Cre activity is controlled by red light by splitting Cre and fusing... Figure 4: The C-terminus of Cre (CreC) and the N-terminus of Cre (CreN) are fused to the truncated Phytochrome... previous Cre/lox or drug-induced Cre systems (ex: tamoxifen). As the name suggests, CreLite combines ...here.        AAV delivery of split base editors Article contributed by Jennifer Tsang AAVs are a great ...transduced at levels similar to that of a single AAV expressing GFP. The lab tested the base editors in vivo ... dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB...proteins separate, inactivating Cre. This is critical for reducing Cre toxicity in long-term experiments...
Showing: 11 - 20 of 93 results