We narrowed to 132 results for: multiplex

...conversion, gives users the flexibility needed for multiplexing. In the case above, you can’t costain with your...antibodies with the same isotype can not be used for multiplexed indirect staining methods since the secondary...the new Fc region. In addition to increasing multiplexing capabilities this process is frequently used...single-plexed experiments, it needs to be addressed when multiplexing. It is imperative that you use narrowly selective...selective isotype-specific secondary antibodies when multiplexing with chimeric antibodies. In the case above ...and mouse 1gG2a primary antibodies used in a multiplexed imaging experiment   We hope that this blog...

...IBEX is a highly multiplexed tissue imaging method that uses iterative staining and chemical bleaching...Radtke et al., 2020, 2022). I do mean highly multiplexed: you can image over 65 different parameters using...an invaluable resource for anyone performing multiplexed imaging, not just IBEX. The IBEX Imaging Community...., & Germain, R. N. (2020). IBEX: A versatile multiplex optical imaging approach for deep phenotyping ...resources on the Addgene blog Antibodies 101: Multiplex Immunofluorescence Negative Can Be Positive: Open...

...lab presented three ways to use CAPTURE 2.0: 1. Multiplex capture of chromatin interactions The Xu lab performed...Long-range chromatin interactions identified with the multiplexed CAPTURE 2.0 largely replicated interactions previously... a large cluster of enhancers. CAPTURE 2.0’s multiplexing and high resolution allowed the Xu Lab to simultaneously...role in gene regulation The high resolution and multiplexed chromatin ‘photos’ generated with CAPTURE 2.0..., Orkin, S.H., Zhang, M.Q., & Xu, J. (2020). Multiplexed capture of spatial configuration and temporal...

...optimizing for LightCycler machines.) Multiplexing It is possible to multiplex qPCR when using fluorescent probe-based...change, the multiplexed protocol may have to be re-optimized.  While troublesome, multiplexing your reference...the Lightcycler while being relatively easy to multiplex (Thellwell, 2000). See Table 1 for a comparison...noise Probe Allelic specificity Can be multiplexed Single amplicon validation technique Hydrolysis... reagents in your chemistry, unless running a multiplex reaction or on a rapid cycle machine. Melt Curves...possible (Vandesompele, 2002), and it's possible to multiplex your reference genes to save on plate space. For...six reactions in a single well. In practice, multiplexing is quite difficult and requires significant ...

...articles.  Here's what you'll find in this post: Multiplexed perturbation and decoding in pooled CRISPR screens...System Neurodegeneration Collection updates   Multiplexed perturbation and decoding in pooled CRISPR screens...screening, Paul Blainey’s lab has developed a multiplexing approach to Cas9-based pooled CRISPR screens...uses two sgRNAs with internal barcodes (iBARs), multiplexed using tRNAs, within the lentiviral 3’ long terminal...reduce recombination and avoid separation of the multiplexed elements. The team designed sgRNAs that include...optimizations produced 90% accurate integration of multiplexed constructs into target genomes and a 10-fold ...2024). CROPseq-multi: a versatile solution for multiplexed perturbation and decoding in pooled CRISPR screens...

...us about a new tool for efficient multiplex base editing and multiplex prime editing (requiring minimal...editing tool for large inserts.  DAP arrays for multiplex gene editing: Guest blogger Qichen Yuan tells ...

...Base editing CRISPRi in Caulobacter crescentus Multiplexed gRNA for human pluripotent stem cells CRISPR-...the paper in mBio Find the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system for human pluripotent... deliver uniform and sustained expression of multiplexed gRNAs in human pluripotent stem cells. Previously... construct and the fluorescently-labeled gRNA multiplex. Read the paper in Scientific Reports Find the...

...as those generated by multiplexed delivery of multiple sgRNAs. Since multiplexing is commonly used to create...edits is critical to the CRISPR workflow. For a multiplex sample, ICE includes visual representations of...visualization helps the researcher see which of the multiplexed sgRNA was involved in a particular edit, and ...can analyze indels that result from single or multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9...the wild type sequence.  If you are viewing a multiplex sample, the cut site will be aligned to the most...

... Depositor Collections MORF Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection...