...IBEX is a highly multiplexed tissue imaging method that uses iterative staining and chemical bleaching...Radtke et al., 2020, 2022). I do mean highly multiplexed: you can image over 65 different parameters using...an invaluable resource for anyone performing multiplexed imaging, not just IBEX. The IBEX Imaging Community...., & Germain, R. N. (2020). IBEX: A versatile multiplex optical imaging approach for deep phenotyping ...resources on the Addgene blog Antibodies 101: Multiplex Immunofluorescence Negative Can Be Positive: Open...

...lab presented three ways to use CAPTURE 2.0: 1. Multiplex capture of chromatin interactions The Xu lab performed...Long-range chromatin interactions identified with the multiplexed CAPTURE 2.0 largely replicated interactions previously... a large cluster of enhancers. CAPTURE 2.0’s multiplexing and high resolution allowed the Xu Lab to simultaneously...role in gene regulation The high resolution and multiplexed chromatin ‘photos’ generated with CAPTURE 2.0..., Orkin, S.H., Zhang, M.Q., & Xu, J. (2020). Multiplexed capture of spatial configuration and temporal...

...optimizing for LightCycler machines.) Multiplexing It is possible to multiplex qPCR when using fluorescent probe-based...change, the multiplexed protocol may have to be re-optimized.  While troublesome, multiplexing your reference...the Lightcycler while being relatively easy to multiplex (Thellwell, 2000). See Table 1 for a comparison...noise Probe Allelic specificity Can be multiplexed Single amplicon validation technique Hydrolysis... reagents in your chemistry, unless running a multiplex reaction or on a rapid cycle machine. Melt Curves...possible (Vandesompele, 2002), and it's possible to multiplex your reference genes to save on plate space. For...six reactions in a single well. In practice, multiplexing is quite difficult and requires significant ...

...articles.  Here's what you'll find in this post: Multiplexed perturbation and decoding in pooled CRISPR screens...System Neurodegeneration Collection updates   Multiplexed perturbation and decoding in pooled CRISPR screens...screening, Paul Blainey’s lab has developed a multiplexing approach to Cas9-based pooled CRISPR screens...uses two sgRNAs with internal barcodes (iBARs), multiplexed using tRNAs, within the lentiviral 3’ long terminal...reduce recombination and avoid separation of the multiplexed elements. The team designed sgRNAs that include...optimizations produced 90% accurate integration of multiplexed constructs into target genomes and a 10-fold ...2024). CROPseq-multi: a versatile solution for multiplexed perturbation and decoding in pooled CRISPR screens...

...us about a new tool for efficient multiplex base editing and multiplex prime editing (requiring minimal...editing tool for large inserts.  DAP arrays for multiplex gene editing: Guest blogger Qichen Yuan tells ...

...Base editing CRISPRi in Caulobacter crescentus Multiplexed gRNA for human pluripotent stem cells CRISPR-...the paper in mBio Find the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system for human pluripotent... deliver uniform and sustained expression of multiplexed gRNAs in human pluripotent stem cells. Previously... construct and the fluorescently-labeled gRNA multiplex. Read the paper in Scientific Reports Find the...

...as those generated by multiplexed delivery of multiple sgRNAs. Since multiplexing is commonly used to create...edits is critical to the CRISPR workflow. For a multiplex sample, ICE includes visual representations of...visualization helps the researcher see which of the multiplexed sgRNA was involved in a particular edit, and ...can analyze indels that result from single or multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9...the wild type sequence.  If you are viewing a multiplex sample, the cut site will be aligned to the most...

...Cis/trans activity, PAM requirement, gRNA, and multiplex ability Experimental guidelines and applications...you need more nitty-gritty details — how can I multiplex this enzyme? What other RNA components are required.... For each enzyme, it will also show you how multiplexed arrays would be designed, if that’s your end ...details to refine your search (trans activity, multiplex ability, gRNA design, etc.). With this feature...

...it except in the most dire of circumstances.) Multiplex assays Mo’ proteins, mo’ problems may not be a...common saying, but it probably should be. In a multiplex assay - one with multiple proteins of interest...different species and/or isotypes. For highly multiplexed assays, using secondary antibodies which recognize...of the number of protein targets, simplifying multiplex assays.  A brief note Since the indirect method...