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We narrowed to 84 results for: pooled crispr library plasmids dna

Showing: 11 - 20 of 84 results

  1. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...use pooled lentiviral CRISPR libraries and keep the following in mind: These libraries are pooled. Most...Two-way CRISPR screening In October, Jonathan Weissman’s lab introduced additional pooled CRISPR libraries... pooled crispr libraries...optimize humans for CRISPRi and CRISPRa.” Before considering the use of pooled libraries in your experiments...Silvana Konermann, the first generation of CRISPR-Cas9 plasmids were not designed with gene activation in... the CRISPR system was a large one.  The question for her then was this: Can you engineer CRISPR-Cas9 ...achieved.” That is, until now. Enter the SAM pooled library In a paper published in Nature in December,...

  2. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ... receiving a pooled gRNA library in plasmid format, a researcher must amplify the library, use next-generation...popular set of tools in the repository are CRISPR pooled gRNA libraries that can be used to screen for genes...publications associated with these libraries. Please note, the pooled libraries we distribute in lentivirus ...receive transfer plasmid DNA that you can use along with lentiviral packaging plasmids to create your own...rough approximations of the total number of CRISPR plasmids for that particular organism available at Addgene...ready-to-use CRISPR lentiviral preps to researchers across the globe. Why provide CRISPR as virus? “Like...Validated gRNAs to use with your Cas9 Virus Pooled libraries now available in lentiviral format Another...

  3. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...Watch the Video! CRISPR Library Amplification Amplify CRISPR pooled-plasmid libraries Diagnostic Restriction...the Video! DNA Ligation Assemble DNA using DNA ligase Bacterial Transformation Introduce DNA into a bacterial...phenol-chloroform extract, and precipitate DNA DNA Quantification Measure DNA concentration with a spectrophotometer...and analyze plasmid DNA with restriction enzymes Agarose Gel Electrophoresis Separate DNA by size on an...Add a short stretch of DNA to a plasmid Gibson Assembly Combine overlapping DNA fragments in a single ...Store bacterial strains or plasmids for long term use Watch the Video! DNA Purification Miniprep, phenol-chloroform...FAQ Watch the Video! Purifying DNA from an Agarose gel Isolate specific DNA bands cut from a gel Watch the...

  4. What's New in CRISPR - September 2019

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    Blog Post
    ...inducible CRISPR/Cas9 activation E. coli genome-wide inhibition library CRISPR knockout libraries for cancer...metastasis CRISPR screen. The Chen Mouse Tumor Suppressor Gene CRISPR Knockout Library is an AAV CRISPR knockout...Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid...Cas9 plasmids E. coli genome-wide inhibition library In the last edition of “What’s New in CRISPR,” we...Bikard E. coli Genome-wide Inhibition Library CRISPR knockout libraries for cancer research The Sidi Chen...published two CRISPR knockout libraries for cancer research. The Mouse Metastasis CRISPR-Cpf1(Cas12a) ...

  5. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post
    ...Specific Mutations in Your Plasmids Learn How You Can Use CRISPR Pooled Libraries to Generate Mutant Populations... Your Plasmids Resources on Addgene.org Find Plasmids from the Whitehead Lab Use CRISPR Pooled Libraries...winnows the pool down to plasmids expressing variants with optimal function. High-throughput DNA sequencing...homologous DNA fragments in a single-tube reaction. For mutational scanning experiments, libraries of DNA fragments...This technique relies on the generation of a plasmid library that expresses all desired variants of a protein...functional score based on its library frequency before selection compared to its library frequency after selection...that can be used to generate complex libraries of mutant plasmids ready for deep mutational scanning. ...

  6. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...23328393 Wu lab human lentiviral CRISPR library     CRISPR pooled libraries enable researchers to screen ...at Texas Tech developed a pooled CRISPR knockout library and used this library to screen for genes involved...KnockOut (TKO) CRISPR Library. This complex second-generation CRISPR lentiviral library targets nearly...PubMed PMID: 26950006 Plasmid ID Plasmid Name Plasmid Type 74309 pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry... Wu lab knockout library is available alongside a growing number of CRISPR libraries from Addgene’s repository...General information regarding how to use a CRISPR pooled library as well as criteria of a good screening ...Drosophila CRISPR library Addgene has had the privilege of distributing several human and murine CRISPR pooled...

  7. The AAVantages of AAV in CRISPR Screens

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    Blog Post
    ...resources at addgene.org CRISPR pooled libraries Guide to pooled libraries CRISPR guide AAV guide ...Limitation for CRISPR Delivery Genome-wide Screening Using CRISPR Performing In Vivo CRISPR Screens Using...development and improvement of CRISPR-based technologies over the past decade. CRISPR screens are easily customizable...supported by the wide array of lentiviral-based CRISPR libraries available at Addgene. The large packaging ...process of these screens is the same: Deliver CRISPR library to cells of interest. Select for cells of interest...vivo starts with transducing cells with the CRISPR library in vitro before transplanting them into mice...screens, mice are directly infected with the CRISPR library. In vivo screens can become costly, so having...

  8. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...Research Find Pooled Libraries for Your Research View our Hot Plasmid Pages to See the Latest Plasmid Technologies... each to make the library usable for pooled screens. Applications of the cancer pathways ORFs kit Wood...How the Clon Tracer Library Has Been Used in Cancer Lineage Tracing Read How CRISPR Can Be Used in Genome-Wide...identified a set of one to three mutant complementary DNAs (cDNAs) representing core nodes that, when over-expressed...literature to curate the perfect library, obtained the appropriate cDNAs from other researchers, and cloned...cloned all the cDNAs into a single consensus lentiviral expression vector and attached a DNA barcode to each... drugs in specific contexts, so he assembled a plasmid kit designed to do exactly that. As described in...

  9. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid...Zhang lab developed a genome-scale CRISPR inhibition pooled library (~60,000 gRNAs) that targets ~4,000... the E. coli genome-wide CRISPR inhibition library Covalent tethering of DNA repair template to Cas9 ...blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources...help you find new CRISPR tools for your research! This time: E. coli genome-wide CRISPR inhibition Covalent...Covalent tethering of DNA template to Cas9 SECURE base editors Nme2Cas9 CRISPR interference in Candida albicans...

  10. Download Science-Themed Zoom Backgrounds from Addgene

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    Blog Post
    ... COVID-19 research, pooled CRISPR lentiirus libraries, pooled barcoding libraries, and more. Download ...lentiviral plasmids include popular envelope and packaging plasmids as well as transfer plasmids. Download...Otherwise, check out our plasmid and DNA backgrounds. Download the blue plasmid background.     You can...the green DNA background.     Since we ❤️ DNA at Addgene, we're presenting you with another DNA background... the plasmids that Addgene offers by visiting our collections page. Here, you can browse plasmids by field...your favorite reagent, scientific technique, or plasmid repository at your next meeting.     If you’re...cute mascots!  Blugene has been helping us make Plasmids 101 videos and needed a break so they're hanging...
Showing: 11 - 20 of 84 results