We narrowed to 91 results for: psin
-
TypeBlog Post...controls: pAAV-hSyn-DIO-EGFP (50457-AAV5): The Synapsin promoter directs broad, neuronal expression. AAV...specific neurons. We recently packaged a soma-targeted opsin from Christopher Harvey’s lab. According to their...
-
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...receptor for SARS-CoV. The lab also found that cathepsins play a role in SARS-CoV infection and deposited...deposited plasmids for the expression of cathepsin S, L, and B. This became a great starting point for SARS-CoV... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post...AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1) targeting parvalbumin cortical interneurons...use viral prep in serotype AAV5. Get the channelrhodopsin here Astrocyte specific tight affinity glutamate... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...kinase II alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal) promoters (Figure 1b), and sgRNAs... Neuron-specific expression is mediated by the synapsin 1 (SYN) promoter, which was superior in its ability... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...Activation of these neurons via the channel Channelrhodopsin 2 (ChR2) reduced glutamate transmission from...mouse to deliver Cre-dependent expression of Channelrhodopsin 2 (ChR2), a modified receptor protein that... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...derived from direct comparative analysis of microbial opsins." Nature methods 9.2 (2012): 159-172.5. Goshen ...Addgene A Primer on Optogenetics: Introduction and Opsin Delivery The Materials Science of Optogenetics Experiments... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post..., Adam Cohen's lab presented Archaerhodopsin 3 (Arch) as a new rhodopsin based voltage indicator for use...plasma membrane. To obtain a more sensitive channelrhodopsin actuator for triggering action potentials ... -
New Viral Vectors - Spring 2025
TypeBlog Post...far-red fluorescent marker, a Flp-dependent channelrhodopsin in the retrograde serotype, and a dTomato ... -
New Viral Vectors - Summer 2024
TypeBlog Post...optoGPCR" (coupled to Go, so it's an inhibitory opsin!) and due to its bistable nature, it enables inhibition... -
Newly Updated AAV Data Hub!
TypeBlog Post...report describes a functional vector such as a channelrhodopsin Images showing the injection site as well ... -
Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies
TypeBlog Post...injected retrograde AAVs at one location result in opsin expression in their cell bodies. Optical stimulation... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog Post...containing plasmids for expressing different types of rhodopsins and sensors as well as references and links to... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog Post...very high, ubiquitous expression, while the human synapsin 1 promoter is neuronal specific (Haery et al.,... -
The Importance of High Titer for AAV Transductions
TypeBlog Post...receptor constructs with AAV. In the case of channelrhodopsins, fewer channel proteins are required for the... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...method allowed Tian to optimize a red‑shifted archaerhodopsin‑derived genetically encoded voltage indicator... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...they wanted, the team identified Archon1, a novel opsin-based fluorescent voltage indicator. Archon1 can... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post... also successfully regulated expression of channelrhodopsin-2, commonly used in optogenetics, opening ... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...the same transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog Post... purified via streptavidin pulldown. Following trypsin digest, PPIs are identified via mass spec analysis... -
Hot Plasmids - October 2020
TypeBlog Post...a Cre-dependent, soma-targeting inhibitory channelrhodopsin. It’s now available as AAV5. Find the AAV... -
Plasmids 101: FLEx Vectors
TypeBlog Post...and Sternson SM. 2008. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range... -
Common Injection Routes in Mice
TypeBlog Post... place the needle in the correct vein without collapsing it. Retro-orbital injections are also thought... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post...line, and its transfection efficiency. We often trypsinize the transfected cells on the day after transfection... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far) or for cancer... -
Hot Plasmids Spring 2024
TypeBlog Post...they determined that an A122D mutation in the rhodopsin domain conferred several new improvements. The... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post... M. Sternson. 2008. “A FLEX Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog Post...blue-light controlled optogenetics, such as channelrhodopsins and tools based on light-oxygen-voltage (LOV... -
Hot Plasmids: Spring 2025
TypeBlog Post...to express either the optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post...Selective promoters Selective promoters, such as synapsin, glial fibrillary associated protein (GFAP), or... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...intracellular Ca++ release with an optogenetic receptor, melanopsin, requiring brief exposure to 470nm blue light...