We narrowed to 151 results for: GFP reporter gene

...your regulatory element or gene of interest into to create a luciferase reporter. Unless otherwise noted,... 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic ...Multiple Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-b, c-Myc...luciferase). Feng Zhang Return to Top Reporter Constructs Already know what gene or regulatory element that you...Explore Addgene's collection of luciferase plasmids for gene expression assays and bioluminescent reporters...Root 105533 pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase and GFP James Wilson...Collection Empty Backbones Expression Constructs Reporter Constructs Other Highlights Additional Resources...

...Synthetic Photobiology: Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used...study of protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria...bacterium. Plasmids containing easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent proteins...Bacterial one-hybrid (B1H) systems also use reporter genes to identify interactions between proteins and... and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...pET28a_T5-ARG1 Bacterial cells Ultrasound (acoustic reporter gene, ARG1) Escherichia coli Nissle 1917 Mikhail ...pET28a_T7-ARG1 Bacterial cells Ultrasound (acoustic reporter gene, ARG1) Escherichia coli Mikhail Shapiro 106474...

... SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) to detect single molecules of RNA or...plasmids Tagging human transcription factors with GFP Kevin White’s lab recently deposited a set of CRISPR...plasmids to tag human transcription factors with GFP. As part of the Encyclopedia of DNA Elements (ENCODE...proteins for optogenetic control of CRISPR-Cas9. By combining AcrIIA4, a Streptococcus pyogenes Cas9 inhibitor... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...adapted for detecting SNPs. Learn more from this Addgene blog post Read the paper in Science Find the...the Cas14 plasmids Anti-CRISPR proteins for optogenetic control of CRISPR-Cas9 Anti-CRISPR proteins that...

...NIR FPs can be used similar to GFP-like probes by supplying a single gene to cells. Still in bacteria, ...molecular brightness (Table 1).  NIR reporters and biosensors NIR reporters can tell us about cellular events...brightness than previously reported widely used dimeric iRFPs (Table 1). In general, the monomeric state of...normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0 was later found..., which do not produce BV, an additional gene coding for heme oxygenase should be supplied (Baloban et...crosstalk-free three-color imaging with green and red GFP-like FPs (Shcherbakova et al., 2016), as well as ...protein-protein interactions, we recommend using NIR split reporters based on miRFPs (Shcherbakova et al., 2016), since...

...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...DsRed1-E5 as a reporter for mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial...Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca 2+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ...maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation process by a novel reporter protein...Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters via electrochromic...Description Gene/Insert Vector Type Eligible for Viral Packaging PI Return to top Other Reporters ONE-GO Biosensors...

...expression of Gene X using shRNA expressed from a plasmid   Figure 1: Expression Level of Gene X The results... level of Gene X in cells treated with Plasmid A is similar to the expression level of Gene X in the untreated...plasmid that constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either co-transfected...backbone itself on gene expression in your target cells. In experiments employing gene targeting or genome...trying to repress a gene, your control might be a system where expression of that gene is knocked out completely... shRNA targeting human Gene X in Plasmid A is the decreased expression of Gene X. Ergo the positive control...targeting human Gene X. The non-targeting control also assesses any effects of the general introduction ...

... developed four acoustic reporter gene (ARG) plasmids to better monitor gene expression in vivo using ...plasmids at Addgene. Liu Q et al., Neuron. 2019. PubMed PMID: 30704911. Acoustic reporter genes for noninvasive...contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment In recent years, scientists ...rely heavily on fluorescent protein genes. These optical reporters have limited deep-tissue performance...demonstrates engineered gas vesicles as the first reporter genes using ultrasound and provide a major benefit...29300010. miRNA reporter vectors shed new light on mechanisms of miRNA-mediated gene regulation Article...collection of reporter plasmids is amenable to Golden Gate assembly for turning the single-input reporters into...

...deletion of one or several of these genes permits the expression of genes farther downstream in the construct... two tandem sets of genes aligned head-to-head and the deletion of one of the gene pairs. Brainbow 2.0... from the default GFP (green) to RFP, CFP, or YFP fluorescence. In Brainbow 2.1, GFP is tagged with a ...recombination in this mouse generates cells that randomly express either nuclear GFP, membrane CFP, cytoplasmic...Brainbow uses a Cre/lox system to label neurons. Genes encoding the fluorescent proteins are organized ...organized in tandem with a single promoter, but only the gene directly following the promoter is expressed. Cre-mediated... which uses Cre to trigger the inversion of two genes aligned head-to-head. Another variation, Brainbow...

... to track where GFP labelled B cells were in mounted slices of these organs. These GFP labelled cells ...enabled by fluorescent proteins The optimization of GFP and its derivatives by the late Roger Tsien changed...stimulus. Using an epifluorescence microscope and GFP fusion proteins controlled by promoters specific ...the immune lineage, scientists could easily track GFP expressing cells. For example, by harvesting lymph...transgenic mice expressing fluorescent protein reporter constructs in the immune lineage. By specifically... the infiltration of neutrophils expressing LysM-GFP (green) into a draining lymph node after infection...using 2-photon microscopy as this hasn't been well reported for many fluorescent proteins. Additional fluorescent...

...the limitations is the size of GFP: ~240 amino acids or about 28 kDa. GFP and its homologues have a beta-barrel...fluorescent protein (GFP) from Aequorea victoria and its variants are genetically encoded fluorescent ...interference is known as steric hindrance. For instance, GFP may occlude catalytic sites or obstruct binding sites...post-translational modifications. Despite these potential issues, GFP has been used successfully in countless fusion proteins... an inert light emitting module, any tendency of GFP to homodimerize should be avoided because it will...the linker sequence short In the early days of GFP applications, many were concerned with steric hindrance...fluorescent proteins derived from Aequorea victoria GFP sticks out and can be considered a linker (figure...

...systems allow researchers to understand bacterial gene clusters that produce useful natural materials, ...capable of robust expression of high G+C content genes, as validated through monitoring the expression ...plasmids use “Cas9-mediated adenosine transient reporter for editing enrichment”, or XMAS-TREE, to identify...coding sequence for a green fluorescent protein (GFP). The success of the A-to-G conversion could be seen...through the success of the change in expression of GFP, for the stop codon should have changed from “TGA...cytometry is used to sort populations by mCherry and GFP expression to check for ABE’s. b) Quantification ...human mRNA binding proteins with 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode...

...molecule it detects. This change then generates a reporter signal. Reporter signals may be electrochemical or...biosensors To focus on a particular gene or protein, check out our gene-specific biosensors. These constructs...light-based, with luminescent and fluorescent reporters being especially popular. We’ll give you an introduction...biosensors as an example. Many calcium biosensors include GFP fused to calmodulin (CaM) and the chicken myosin ...glutamate sensor pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget about the other end of the biosensor...Visualization Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent... OR enzyme activity. Small molecule biosensors Addgene has many sensors that measure the concentration...

...among other things) gene delivery, generating pluripotent stem cells, and developing gene therapies for monogenic...and lentiviral vectors that are used for gene regulation, gene of interest expression, knockout, and screening...gamma-Retroviral Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for...for cloning and expressing your gene of interest with GFP expression.  Cloning and expressing RNAi pMKO...technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral vector from the Gage lab encodes GFP and Cre-recombinase...Experimental uses include: Cre-dependent gene expression, gene knockout, and selection marker removal. ...Expressing your gene of interest pLenti-puro (Plasmid #39481) You can clone your gene of interest into...

...epitopes, such as GFP (Paix et al., 2014). A further development of this approach reported in 2017 uses the... Mello lab also reported that modifying the 5’ modification of oligos used to generate linear dsDNA repair...where his group focuses on gene regulation in nematode molting and spermatogenesis. His group also builds ... one of the model organisms which I use to study gene regulation during developmental processes, CRISPR...drug resistance (Chen et al., 2013), fluorescence (GFP; Tzur et al., 2013), or rescue of mutant phenotypes...Craig Mello’s lab – used inactivation of the unc-22 gene as their selection marker (Kim et al., 2014). Meanwhile...approaches may become even more powerful following a report that one can use linear repair templates (ie. PCR-derived...

...stem cells By Jennifer Tsang CRISPR gene interference (CRISPRi) and gene activation (CRISPRa) are great ways...GABA-ergic interneurons. These include mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato, mDlx-GCaMP6f, hDlx-GiDREADD-dTomato...into the hPSC genome using AAV1 and contain an EGFP reporter. Expression of the dCas9-KRAB activator or dCas9...the plasmids at Addgene! A new tool for synthetic biology uses CRISPRi to control gene expression. This...nucleus and modulate transcription of the mCitrine gene. mCherry is constitutively expressed. Cells are ...selectivity and found that the best motif was from the Lck gene. For marking microtubules of mammalian cells, the... ways to modulate the expression of nearly any gene via specific guide RNA (gRNA) sequences. But these...

...viral replication and transcription as well as reporter genes. To learn how you can use minigenomes in your...repression of your target gene, activation and repression of different genes in a single cell, and localization...Favorite Fluorescent Protein Oligomerizes in Cells GFP oligomerization can lead to misleading artefacts ...Lab at MIT developed plasmid based systems that report the order in which specific chemical inputs are...recombinase-based state machines could potentially be used to report upon and/or engineer “temporally ordered biochemical...derived from Zika virus and, upon binding, produce reporter proteins. These toehold switches can be used to... them in action. AAV: A Versatile Viral Tool for Gene Expression in Mammals Adeno-associated viruses (...

...nature of GFP fluorescence to create an improved fluorescent aptamer system. Knowing that GFP’s  structure...Protein Activity with GFP Resources at Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids...Resources on the Addgene Blog Tips for Using FRET in Your Experiments Learn more about GFP Controlling Protein...highly customizable in terms of how they actually “report” as biosensors. Making aptamers glow The flexibility...increase its fluorescence, essentially mimicking GFP.  Though the aptamer, called Spinach, required longer...and used in assays for over half a century; the Addgene blog has an excellent primer and tips from experts...can be created in many different ways, but the general premise is that an aptamer in its unbound state...

...-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoter. 8 Alexander Dityatev...lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter. 8 Alexander Dityatev...Cre-dependent Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby for labeling of axon terminals...dependent manner. 1 Don Arnold 124364 pAAV-FLEX-DTR-GFP CBA-driven, Cre-dependent Cre-dependent expression...expression of diphtheria toxin receptor fused to GFP for studying cell ablation. 2 Thomas Jessell , Eiman Azim...Long Overexpression Tools These AAV encode various genes for overexpression. ID Name Expression System Activity...glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses infected in postsynaptic cells. 5 Don ...

...irreversible PA-FP reported was PA-GFP. PA-GFP was derived from Jellyfish (Aequorea victoria) GFP (wtGFP) by mutating...mutation in PA-GFP generates a chromophore population that is more neutral, causing PA-GFP to display almost...example, by engineering PA-GFP tagged to a mitochondrial matrix protein (mito-PAGFP) the Youle Lab was able...Multi-Color Imaging Catch Up on Your GFP Background Resources at Addgene.org Find Photoactivatable FPs in ...the neutral to the anionic form, which allows PA-GFP to exhibit up to a 100-fold increase in fluorescence...when illuminated with blue light (1). Similar to PA-GFP, scientists later developed red fluorescent PA-FPs...in vivo imaging models perfer the PA-RFPs over PA-GFP. Another class of irreversible PA-FPs are the photoconvertible...

...viral prep pAAV-CAG-GFP AAV9-X1.1 Controls Edward Boyden New serotype pAAV-hSyn-EGFP AAV PHP.eB Controls...services collection or check out Addgene's Data Hub to see viral vector reports from other users. And don't...AAV9. You can now target these cells with AAV-CAG-GFP packaged in AAV9-X1.1!  We also have eight new AAV-PHP.eB...preps available in the Addgene viral vector repository! This includes the first Addgene prep packaged in the... AAV1 Optogenetics Karl Deisseroth New serotype pAAV-hSyn-hChR2(H134R)-EYFP AAV2 Optogenetics Karl Deisseroth... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...other new AAV vectors as well — including next-generation biosensors for GABA, dopamine, serotonin, and...

...levels for these two reporters simultaneously. By fusing sfGFP and/or mCherry to genes of interest, it is...expression of two genes without cross‐talk. (A) Schematic of the strain expressing two reporters and dCas9 under...attachment sites. The levels of the two reporters, mCherry and sfgfp in this case, can be controlled using...to bind on the chromosome. When dCas9 binds to a gene, it blocks the progression of the RNA polymerase...enables robust, noiseless knockdown of bacterial genes. Molecular Systems Biology, 14(3):e7899. doi: 10.15252... the tracrRNA sequence (not shown). (B) Relative GFP and RFP concentration given relatively to the non‐targeting...carrying two CRISPR guides. The first one binds to sfgfp with either 0, 10, 11, 14, or 20 matching nucleotides...

...sequence (UAS) of its target gene, in this case a reporter gene (e.g. luciferase or GFP), regardless of whether... promoter of the reporter gene to activate expression (Figure 2c). The reporter gene then allows detection...rescue genes as reporters and some that repress instead of activate transcription. The HIS3 reporter system...co-expressed in a yeast strain that also contains a reporter gene cloned downstream of a promoter containing the...to as the “bait”, Figure 2b) cannot activate reporter gene expression on its own, but, if the two POI’s... activator, bacterial LexA DBD and the lacZ reporter gene (3, 4), there are now many variations, including... to reduce the likelihood of indiscriminate reporter gene activation. Including a prey-only control also...

...vitro and in vivo for gene expression and/or function. These data are sometimes reported or posted on the ... later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the...genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (Addgene 84445-AAVrg) alone at 1.1E6 vg/mL, or both...Karl Deisseroth (Addgene viral prep # 55632-AAVrg). pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] was a gift from... plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral helper sequences...recipient's initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit...

...targeting a gene associated with diphtheria toxin lethality and were sorted into single wells to generate clonal...PubMed. Find the Plasmids @Addgene: iTOP-tested proteins and reporters Previous Protein Delivery ...instance, it’s much more difficult to introduce a gene of interest into primary cells, so most primary ... cells and activate expression of a luciferase reporter. CPP was not required for protein entry into cells...primary cell types using Cre and various loxP reporters. In both mouse and human embryonic stem cells,...Neuronal stem cells transduced with iTOP resulting in GFP expression. Image courtesy of Diego D'Astolfo. ... risk of viral integration causing mutations and gene expression changes elsewhere in the genome. Second...

...Adeno-Associated Viral Vector-Mediated Gene Delivery to the Hypothalamus. Human Gene Therapy, 21(6), 673–682. https...conditions commonly encountered in human gene therapy trials. Human Gene Therapy Methods, 26(2), 71–76. https...neurons and GFP (green) to label neurons transduced by each serotype. Working at Addgene has shown ...It is a curated set of technical reports on AAV vectors from Addgene and the CLOVER Center, each one submitted...submitted by researchers like you. Reports are reviewed by Addgene scientists for clarity and accuracy... cassette with an ubiquitous promoter to express GFP in a group of young rats. We found that, in this ...Grames, M. S., & Klein, R. L. (2018). More expansive gene transfer to the rat CNS: AAV PHP.EB vector dose–...

...imaging with this set are mTagBFP2, EGFP or one of the improved GFP variants, mRuby2 or TagRFP-T, and an...with this filter set and have reported brightness measurements. Here, EGFP outperforms the improved folding... / em 527 nm) can be imaged together but CFP and GFP (ex 488 nm / em 507 nm) show some crosstalk between...TagRFP to give a brighter signal than mCherry. In general, filter sets designed for Rhodamine / Cy3 will ...mammalian cells, one of the improved folding variants of EGFP like mEmerald or Clover is probably best; mNeonGreen...set of proteins the green and red proteins are generally the most detectable and so should be used to tag...

...permuted GFP (nLightG/nLightG2) or mApple (nLightR/nLightR2) for green and red fluorescence reporting, respectively...Image from Morano et al. 2026. Addgene Report https://doi.org/10.57733/addgene.6tylmq reproduced under CC ...unfortunately, nLightR2 is incompatible with some reporters or optogenetic tools due to photoswitching upon strong...pairing. Check out the antibody page and Data Hub reports to learn more and see if it's the right tool for...Inspired by recent improvements on another GPCR-based reporter for dopamine, GRABDA3m, Rohner et al. tested a... a variety of mutations in the nLight reporter domains. The resulting constructs, nLightG2 and nLightR2... (magenta) and nLightG2 (green) rAAVs, showing reporter expression and optic fiber location (dashed lines...

...lines and are not used for general plasmid selection. Instead, reporter genes are often employed to easily...test for your gene of interest may be another method to assess transfection success. GFP is often used...expression levels in the cells. Ideally, the chosen reporter is unique to the cell, is expressed from the plasmid...used as a reporter and we will be covering this ever-versatile fluorophore in a later post, so stay tuned...conjunction with positive selection to ensure your gene gets targeted to a specific location within the ...used selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action...use G418. This can be confusing since the neo/kan gene confers G418 resistance; however, like gentamicin...

...fluorescent protein reporter under the control of promoters that control cell cycle gene expression.  Additional...Resources on the Addgene Blog Visit the fluorescent protein topics page Get the basics on GFP Find microbiology..., Bernach plans to use a suite of fluorescent reporters. Bacteria containing unstable fluorescent proteins...colonizer, labelled with a constitutively fluorescent reporter can also be used to track how one population responds...work in Lin Tian’s lab at UC Davis is to generate a genetically encoded fluorescent sensor for serotonin... the TIMER growth rate bioreporter. During protein maturation, the bioreporter spontaneously changes from...sequence an individual’s genome. But when they find genetic variants, what does that mean? Previous research...

...fused to GFP forms fluorescent biomolecular condensates. A GFP-nanobody fused to the killswitch (GFP-nb–KS... killswitch can be genetically fused to a protein of interest or targeted to a GFP fusion protein using...Grelet Lab developed MitoTRACER, a retroviral-based reporter system that permanently labels the cells receiving...using an anti-GFP nanobody (Figure 4). Once incorporated into a condensate, the killswitch disrupts its ...CRISPRa components to adipose cells to activate genes involved in converting white adipose tissue (WAT... metabolizing glucose to dissipate heat. Of the genes they tested, activating UCP1 had the most potent...increasing oxygen consumption and upregulating other genes normally expressed in BAT. When donor-derived CRISPRa-AAV-treated...

...capture module (SpyTag-GFP nanobody) assembled on beads. C) Micrograph of H1.8-GFP nucleosomes collected...Prime-editing-Assisted Site-Specific Integrase Gene Editing (PASSIGE), their previously-reported technique for precisely integrating...editing, this epigenetic strategy doesn’t induce DNA damage or permanently alter a gene product; it simply...In MagIC-cryo-EM, target proteins (expressed as a GFP fusion in this proof-of-concept study) are captured...SpyTag-SpyCatcher linkers and spacer proteins ending with a GFP nanobody (Figure 1). This enrichment streamlines ...) 3D model of the obtained structure of the H1.8-GFP-bound nucleosome. Images adapted from Arimura et ...). Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases...

...both introduce genes into an organism’s genome (see figure) and to disrupt endogenous genes at the site ...puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain... fused to a region of the RPL41 gene, to drive the selection/reporter parts of each construct. This new...first-generation transposase (as determined by transposition assays of antibiotic-resistance genes conducted...). In their Nature Genetics paper, the lab showed that efficiencies of stable gene transfer using SB100x... the rapid generation of stable transgenic cell lines which can robustly express your gene-of-interest... exciting tool for producing specific genetic mutations and gene disruptions without the use of viral ...

...and a mCherry reporter downstream of the PBAD promoter. To use this plasmid with your gene of interest,...the DNA-binding domain of AraC using a mCherry reporter downstream of a PBAD promoter. While none of the...interest, you would replace mCherry with your gene of interest. pBLADEONLY: This plasmid contains only BLADE ...already have a pBAD33-based plasmid expressing your gene of interest, all you have to do is co-transform ...co-transform pBLADEONLY. That way, a previously cloned gene of interest, under the pBAD promoter, can be controlled...strain transformed with pBLADE expressing superfolder GFP instead of mCherry under the PBAD promoter. They ... Resources on Addgene.org Check out the Molecular Biology Reference Download Addgene's Plasmids 101 eBook...

...popular studies involve using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. pPD80_08) and following...applications used to study gene function. From making transgenic animals, gene targeting and genome modifications...modifications to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g. pLKO.1 - TRC cloning vector). ...These vectors allow you to test the function of Your Gene Of Interest (YGOI) in a controlled environment under...design. You can create a new strain with a disrupted gene by transforming wild-type yeast with an integrating...visualize YGOI in vivo (try pcDNA3-EGFP). If you can get away with generating transiently transfected cells... More Plasmid Resources from Addgene: Plasmids 101 blog series Addgene's Molecular Cloning Guide & Other...

...GCaMP6 family of sensors are commonly used calcium reporters with different kinetic parameters. There are three...ones? Many of the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free...also available. Detection methods Many calcium reporters use a single fluorescent protein (FP), requiring...unwanted variables. Twitch is a FRET-based calcium reporter derived from troponin C. It is engineered with...for in vivo studies) There are several calcium reporters specially designed for the aims and requirements...Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral Services...calcium sensors. Selecting a calcium sensor Genetically encoded calcium indicators (GECIs) are fluorescent...

...of where the genes were expressed. What’s the use of stable gene expression? Constant gene expression can...Researchers express genes of interest from plasmids in order to study gene function or to engineer cells...input and gene expression is the output. The input, plasmid copy number, directly influences gene expression...buffered against the effects of copy number on reporter gene expression. On the other hand, using a constitutive... varied in size or carried different genes. In these cases, gene expression also remained stable regardless...media and growth stage can both affect gene copy number. Moreover, gene copy number is affected differently... constitutive promoter upstream of GFP increased expression of GFP six-fold, but a stabilized promoter...

...to induce high levels of gene activation for both reporter and endogenous genes. It is highly specific ...anti-FLAG-FB-GFP binding to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-GFP labeling...between MARCH5 and WSB2 genes using Cas12a’s ability to knock out multiple genes in parallel. Lastly, this...cellular targets Optimized genetic screening technique using Cas12a for multiplexed gene perturbation The Penn...10846-1.   Optimized genetic screening technique using Cas12a for multiplexed gene perturbation by: Gabrielle...many CRISPR-based genetic screening methods have primarily used Cas9 to knock out genes, researchers at ...multiple genes within a single cassette provides increased simplicity for combinatorial genetic screens...

...limits the delivery of larger transgenes, such as base editors which have a gene size of ~5.2 kb. To overcome...Ace-mScarlet voltage indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed PMID: 31685893. A MoClo...Article contributed by Jennifer Tsang AAVs are a great gene therapy delivery mechanism, but their small packaging...levels similar to that of a single AAV expressing GFP. The lab tested the base editors in vivo and achieved...dosage comparable to those currently used in human gene therapy trials. They further tested the base editors...voltage indicators, however, fall short compared to GFP-based sensors in terms of response and brightness...expression levels from different promoters, and inhibited gene expression using CRISPRi. The kit functions in the...

...has dubbed these fusions LumiFluor Reporters. The LumiFluor reporter constructs were designed to be packaged... bright enough to detect endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc...imaging tools generate cyan, yellow, green, orange and red fluorescence. The paper reports a wide variety...photoactivatable, optogenetic accuators to depolarize or hyperpolarize membranes. Traditional FRET reporters could...would also activate the optogenetic accuators. The researchers took a FRET reporter and adapted it into a...Fusion Protein, codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc® Luciferase are available...couples NLuc to fluorescent proteins to make better reporters and the second couples NLuc to fluorescent proteins...

...Serotype Testing AAV These AAV encode fluorescent reporters and can be used to compare the tropism of different...AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV in...plasmid 37825 (deposited by Edward Boyden ) and direct GFP expression from the CAG promoter. For information...Ready-to-use AAV available from Addgene's viral service. Control EGFP vectors in various serotypes for... 37825-AAVrg.T 20 µL $ 158 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description : Ready-to-use AAV ...plasmid 50465 (deposited by Bryan Roth ) and direct EGFP expression from the human synpasin promoter. For...