...to one or a subset of organisms. Examples: “organism agnostic”, “model organisms”, “small genome”, “large...require the organism to be specified and are truly organism agnostic. Other tools require an organism to be ...only one, or a subset of organisms. In only a few cases the software is organism agnostic. Most tools are...tools will be marked as organism agnostic and have currently available organisms listed in this section...input types can be readily converted.  Examples: “organism”, “sequence”, “identifier”, and “load”. Trends... tools also requiring that the user specify an organism. Output diversity: Different tools provide results...large genome”, and “organism biased scoring”. Trends: Many tools can handle genomes of any size and are...

...Resources Resources on Addgene.org Addgene viral services Addgene Biosafety Guide Addgene Viral Plasmids ...from organization to organization, so you’ll need to be familiar with your specific organization/department...-2 labs. When working with lentivirus, many organizations require or recommend disposable gowns over your...cabinet, even if it is not required by their organization. It’s always okay to add another level of protection..., 10% bleach, or 1% iodine are useful. Some organizations or cities may require a higher percentage of...cut. Following all sharps procedures for your organization will significantly reduce the risk of an accident...also need to familiarize yourself with your organization’s reporting mechanisms before you start an experiment...

...responsibilities Being a leader in an organization, you need to make sure the organization speaks in “one voice” whilst...great organization to develop these leadership skills in particular because every year it organized a “Committee...acquire during their PhD and postdoctoral training. Addgene executive director Joanne Kamens has written a ... achieved by volunteering for a professional organization or a scientific society in your field. My leadership... in the long run was most beneficial for the organization as a whole. I came to understand that not only...only was I growing as a leader but the organization was growing too and with that would come necessary... had to be professional as I represented the organization, not just myself as a scientist. I learnt to...

... Resources on Addgene.org Learn how Addgene engages with the community on our Addgene gives back page...different organizations and individuals, to identify ways of collaborating, and to organize events that...professional development (PD) workshops for teachers; organizing Congressional briefings in Washington DC; convening...Hollywood; and working directly with community organizations, libraries, youth groups, and faith institutions...studies (STS). I also worked with a number of organizations in public engagement efforts, including giving...middle and high school students. One of these organizations was pgEd. After obtaining my PhD and working...audiences. Many universities or cities have organizations (often volunteer-based) that put on public lectures...

...Collection are sorted by organism (see screenshot above). Don’t see your favorite organism listed? Help us serve...us know about other organisms we should include by emailing us at help@addgene.org. If you are looking...Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be... and say hello! Just in time for this meeting, Addgene is launching a new resource page for the Microbiology...plasmids used in E. coli and yeast, you’re in luck! Addgene has so many of these resources that we created ...to narrow your keyword search to one of these organisms. Help us continue to develop resources for your...technology, sign up to write about it on the Addgene blog. ...

...Conjugation, and Transfection Resources on Addgene.org Addgene's Synthetic Biology Collection...Here at Addgene, we often refer to plasmids as lab or experimental tools. They certainly are very handy... a variety of functions that allow their host organisms to thrive in competitive environments. The genes...the competition, and Penicillium isn’t the only organism that knows it. Some bacteria possess plasmid-borne...encode genes that allow the host to infect other organisms. If you’ve ever wondered why E. coli is safe to...means to deliver DNA and other molecules to host organisms. For instance, vectors derived from the Agrobacterium...like by living where no one else wants to. Some organisms manage this with plasmids that allow them to eat...

...post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates. Scientists...conference here. The Future of Research conference is organized by postdocs for postdocs. I am delighted to see...importance of the topics to be discussed, the organizers are building valuable skills and meeting new ...Students also partner with independent career organizations to get the grants and programs they need such...offices in the last few years. However, some organizations are woefully behind and some just don't have...Symposium on the Future of Science: A postdoc-organized symposium on the future of the scientific endeavor...

...path.   Yeast as a model organism Yeast are single cell, eukaryotic organisms formally classified as fungi... Additional Resources on the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors ...know that yeast is a powerful eukaryotic model organism – its genome is easy to manipulate, it’s affordable...cerevisiae (budding). Yeast make for an excellent model organism for a few reasons: Affordable to culture and... methanol metabolism occurs in methylotrophic organisms. K. phaffii is an excellent model for peroxisome...et al., Komagataella phaffi as Emerging Model Organisms in Fundamental Research. Front. Microbiol. 11-...studies Peroxisomes are small, membrane-bound organelles essential for sequestering certain enzymes and...

...nonprofit" organization what do you think of? Maybe the term brings to mind a social service organization like...pantry or community arts organization. Many people are surprised to learn that Addgene is officially filed,... at Addgene If you’re ever curious what specifically drives the decision making process at Addgene, just...in the community Addgene fosters a culture of respect, collaboration, and fun Addgene believes there is... on the Addgene Blog Learn about Our Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports...Our Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...partnerships with organizations that share our community-oriented values. Addgenies like the nonprofit...

...seller. However, Addgene is a bit different from other vendors. As a nonprofit, Addgene does not own the...Anyone affiliated with the recipient organization can create an Addgene account and place an order: a purchasing...through your organization in this field. Please also enter the billing address for your organization. We will...here at Addgene. If you’ve called our customer service line or sent an email to help@addgene.org, you’ve...plasmids online is a little trickier -- but at Addgene, we try to make the process as smooth and simple... bigger question: How does a customer place an Addgene order using a PO number? Why do I have to order...distribute it on behalf of depositing institutions. Addgene cannot fulfill orders based on PO documents that...

... Here’s how Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!)... on collaboration (it’s the heart and soul of Addgene’s mission), but legal complexities can arise when...institutions require them for material transfers. When Addgene entered the plasmid sharing space, we heard over...confidence. Benefits of the UBMTA: Peace of mind Addgene has adopted the terms of the Uniform Biological... for more details. Benefits to the provider (organization / lab that owns the material) Affirmation ...materials will only be shared with non-profit organizations Assurance that recipient may not use the ...Limited liability Benefits to the Recipient (organization / lab receiving the material) Permission ...

...reproducibility workshops and organizational skills. Meanwhile, Addgene was also thinking about ways to...In 2017, Lenny Teytelman, CEO of protocols.io, organized a panel to discuss reproducibility issues in research... there were other academics and like-minded organizations with similar ideas who were also thinking about... an eLife ambassador, had previous experience organizing open access events and grassroots efforts. After... to Teytelman’s connection with Joanne Kamens, Addgene also had the opportunity to contribute to this ...anyone could present the material. For example, Addgene’s contributions by Tyler Ford included reagent sharing... in the year and in the fall of 2018, I helped organize a workshop for community scientists at the Global...

... first hear about Addgene?  I heard about Addgene from Joanna Brownstein, an Addgene board member. When...resources on the Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe...Subscribe to the Addgene blog Resources on addgene.org: Meet the other members of Addgene's Board of Directors...We’re excited to welcome Julie Cicalese to the Addgene Board of Directors. Cicalese served as the Chief...experience and insight to the now 13 member board at Addgene. Let’s meet the newest board member and get to ...School, Joanna was my colleague. She mentioned Addgene was looking for someone with HR expertise to join...serves as an objective body to oversee some of Addgene’s compensation decisions. It was a great time for...

...as a free event! Organizing a science conference for the public How does one organize a public scientific... Here at Addgene, we’re dedicated to advancing and sharing science! In association with the Harvard graduate... graduate student organization Science in the News (SITN), we recently sponsored a first-time event called...communication skills and engage with the public. The organization runs a wide variety of programs, including fall...terms of cost and content level. Sponsorship from Addgene and Harvard Integrated Life Sciences (HILS) made... do you share your science with the community? Addgene would love to hear about your efforts to engage...opportunities to improve our communication and organizational skills provide great training for a number ...

...Popular Model Organisms by Alyssa Cecchetelli and Lukas Morgan There are many model organisms people are...proteins at Addgene. 7. Designing Your Chalk Talk for the Academic Job Interview by Erik Snapp Addgene depositor...2020! Additional resources on the Addgene Blog Read other Addgene news Find the most recent plasmid ...posts Resources at Addgene Browse the repository Find educational resources at Addgene Search for plasmids...discovery. We’ve documented many new tools on the Addgene blog and have created and updated guides on various...interview including defining your research vision, organizing your vision into a chalk talk, and tips for practicing.... In this blog post, we’ve covered five model organisms: mouse, fruit fly, yeast, zebrafish, and worm....

...easily deliver their “cargo” to target cells and organisms, and when that cargo is designed to make you sick...biological tools In nature, viruses infect cells and organisms (which we refer to as hosts) via viral particles...and fuse with. In nature, this determines which organisms and cells they infect. In the lab, this means ...can also be directly injected into hard-to-reach organs, such as the brain. Secondly, some viruses have... makes them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward...strong immune response when introduced into an organism. This feature makes them an attractive tool for...          Click Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration...

...Why have zebrafish become such a popular model organism? Zebrafish (Figure 1) were initially recognized...reasons that zebrafish have been embraced as a model organism, I’ll outline a few of the available genetic resources...reason for the success of zebrafish as a model organism is its amenability to genetic manipulation. Tools...Europe, and China. In addition, the Zebrafish Model Organism Database ZFIN actively curates information on ...Generating zebrafish transgenics Creating transgenic organisms by introducing foreign DNA into the genome is ...fertilized zebrafish zygote transforms into an organism with a clearly established body plan including...pinhead—contribute to the “sculpting” of tissues and organs during embryo development. Single cell sequencing-based...

...body is made up of organs, cells comprise compartments and structures, called organelles. Take a sneak peak...fluorescent microscopy - even in living cells and whole organisms. You can take advantage of the many Fluorescent...pathways, it is possible to map the internal organization of a cell. This mapping can be done by using antibodies...constructs you create in your own work available to the Addgene community. Download our Markers of Subcellular ... FRET Microscopy. Additional Resources on the Addgene Blog Learn More about Plasmids for Endogenous ... proteins” which are known to be a part of an organelle or involved in intracellular transport pathways...for commonly used markers for major mammalian organelles and transport pathways. It is best, though not...

...more. Being that this is the Addgene Blog, I’ll, of course, mention Addgene as a source of sequenced and...blogs - both hosted by individuals and by organizations like Addgene (a good example is our series of blog... with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility. The minisymposium...event, I gave my own talk on reproducibility at Addgene and here I summarize what I learned both in preparation... additional resources and information about organizations promoting reproducibility in this booklet (which...31 - 49:45 - Reagent Sharing - Susanna Bachle, Addgene the nonprofit plasmid repository 49:45 0 1:18:45...within a published manuscript. Luckily, many organizations have been working to provide tools that make...

...another successful event! Addgene company culture At Addgene we organically developed the philosophy of...working at Addgene?” Most Addgenies reply with an enthusiastic, “Our company culture! Addgene is the best... Benjie Chen co-founded Addgene in 2004. They worked tirelessly to get Addgene from a concept to an up... culture here at Addgene has remained paramount. Candidates who interview at Addgene frequently ask the...ideas and company. The Addgene summer picnic This past weekend was the annual Addgene Summer Picnic - a time... happened naturally when Addgene was a family affair back in 2004. As Addgene has grown, it has taken ...Use it! 8. Organize If you’re doing an event that requires people to be in groups, organize your troops...

...away and here at Addgene we couldn’t be more excited! As a small, tight-knit organization we thrive on 3...this Friday will combine all of these. Addgenies have been organizing Halloween festivities for years – searching...email system.  Our 2013 winners dressed up as Addgene's favorite lunch... a BonMe noodle meal, crammed...procrastinating choosing their costume?  As our organization has grown, we continue to have an amazing turnout...interested in finding out who wins this year’s Addgene costume contest, like us on Facebook and checkout...  addgene...

... development. These are required for a growing organism to make the complicated transition from a clump...dynamic processes are largely absent in adult organisms, where tissues structures are more stable and ...movement from the bone marrow and other lymphoid organs to specific tissues where they can scan for any...is large scale cellular movement throughout an organism. Studying cell mobility within these complex...tools to directly follow immune cells in lymphoid organs. The development of cell sorters together with ...antibodies made it possible to track cells from one organ to another. Thus several labs were able to decipher...microscopy images of chemically coloured lymphoid organs did in the past. How do researchers track fluorescently...

... the organization of the nucleus in space and time, and to understanding how nuclear organization varies...varies across the life of a cell, or how organization may be altered in disease states. dCas9 gets a colorful...multiple protein molecules (and also available from Addgene!) Since the signal level provided by SunTag is ...work to increase our understanding of chromatin organization. This fluorescent CRISPR/Cas9-based method is...pantheon of useful CRISPR/Cas9 techniques, we at Addgene are excited to see what’s coming next! References.... Find the plasmids from this publication at Addgene. Dynamic imaging of genomic loci in living human.... Find the plasmids from this publication at Addgene. A protein-tagging system for signal amplification...

...Ready-to-use AAV available from Addgene's viral service. Engineered systemic constructs from Viviana ...As part of our Viral Vector Packaging Service , Addgene is distributing ready-to-use viral preparations...target cell type or tissue of interest, and model organism under study. *Many engineered AAVs for mice use...depth descriptions of each serotype, check out Addgene's blog on Systemic Capsids . To review performance...data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER Center’s repository...produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005) . Note on PHP.eB tropism and specific ... produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006) . Browse Available PHP.S AAV ID Name Promoter...

...nonprofit or U.S. governmental research organizations, and organizations that require the H-1B employee to ...entity, or nonprofit research organizations or governmental research organizations. Since there is no numerical... a Good Cover Letter Resources on Addgene.org Careers at Addgene Global Plasmid Distribution ...Additionally, since most universities and non-profit organizations do not have the April 1 H-1B queue process or... or quota like industry, these organizations can be a good choice for individuals needing sponsorship.... in your field. Membership in a professional organization – this is not referring to one that you can ...program self-driving cars, tissue engineering for organ replacement).  In addition to presenting your expert...

...McKenna and Bert W. O’Malley. Survival of all organisms depends on efficient energy maintenance - through...nervous system signaling to peripheral effector organs, which then return information such as nutritional...processes are regulated at the cellular, tissue and organ level by nuclear receptors (NRs), their coregulators... of such signals between central and peripheral organs because of their established roles as molecular...context defined by ligand, cell type, and promoter, organizing the expression of functional groups of genes ...sections of the current NURSA website. NURSA and Addgene The great progress that has been made in the NR...literature and acquiring them in a timely manner. Addgene represents an excellent solution to the problem...

...professional works for or with an organization to evaluate the organization’s grant readiness, identify grant...stories about a time when their passion for an organization or a program and a funding opportunity coincided...submitting it, to evaluating the capacity of an organization to receive and manage funds.  Grant professionals...targeted to the grants profession? The number of organizations, both public and private, that qualify for grants...oriented nonprofits. Like Deb, I worked for an organization in a position that required some involvement...your ideal client or job will save you and the organization(s) you serve time and resources. 3. Get a mentor...Additional Resources Read other Career Posts on the Addgene Blog! ...

...them paralyzing an organization. I think these are things that I can bring to Addgene -- that comfort with...We’re happy to introduce the newest member on Addgene’s Board of Directors, Bart Newland. Newland is General...all. Q: How does your experience help you as an Addgene board member? BN: Having seen so much in the business...of a lawyer can bring a new perspective to an organization. I'm used to thinking about the downstream results...that business experience and judgement to the organization and see how I can help. Q: What do you enjoy...

...on the Addgene Blog Learn About Experimenting in an Open Source Lab Resources on Addgene.org Try the... is essential to stay in touch with the living organism I study, to be able to see it all scales. Most... a way to better understand and appreciate the organism, for both scientist and non-scientist alike. “...inner structures of delicate plants and other organisms without destroying the original, which could provide...Do you look for the beauty in the microscopic organisms on your lab bench? Let us know in the comments...

...post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates. Panel...result of the content. Here are some of my tips to organizing and running a memorable panel discussion. Check...make it look effortless to the audience, but the organizers and moderator should have a well-planned approach...panelists to the moderator. The moderator and organizers should develop a list of 4-6 pre-event interview...interview questions. The moderator (possibly with an organizer) should set up a 30 minute call with each panelist...example, at a recent event on nonprofit science organizations, I asked audience members to stand and describe...networking time. Both the moderator and program organizers can help make sure the event stays on schedule...

... (XFP) of different colors to label an organ or entire organism. Because each cell can have multiple copies...fluorescent proteins topic page Resources on Addgene.org Browse Addgene’s fluorescent protein collection Learn...The ability to track single cell dynamics at the organism level has been made possible by tools that allow.... Genes encoding the fluorescent proteins are organized in tandem with a single promoter, but only the...developed for tracking various cell types and organisms and for improving the level of detail possible...that allow for multicolor labeling of an entire organism or a specific tissue. XFPs are introduced using...systems allow single cell dynamics in a variety of organisms to be observed in more detail than ever before...

...Everyone workshop at TAGC with the three other organizers. We asked them about their experiences as they...Reproducibility for Everyone Workshop, Abitua and the other organizers found several avenues to keep the audience engaged...conference was over. Depending on the conference, organizers may make the poster available after the conference...actively reach out. It’s more proactive versus organic networking,” she says.Depending on the meeting,...conference format. Additional resources on the Addgene blog Read all of our blog posts about conferences...scientific conferences have followed suit. Two Addgenies, Susanna Bachle and Yijun Zhang, recently attended...conferences (TAGC and ASGCT, respectively) and Addgenie Angela Abitua hosted a virtual Reproducibility...

...Viral Services Resources on the Addgene blog Viral Production at Addgene Viral Vectors 101: What is a Virus...Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene Viral...   Is there an easier way? In fact, there is! Addgene has a number of ready-to-use viral preps available...depends on two things. First, what type of cell or organism context you are targeting? Second, what is the...moderate levels. With regard to target cells or organism, some primary cells and differentiated cell types...shine is when a specific cell type within a live organism is the target. Although rarely done, viral pseudotype...for a type or subtype of cells. This allows an organism to be injected with a virus, but enables tissue-specific...

...Addgene Executive director, Joanne Kamens, recently participated in a Reddit AMA (short for “Ask Me Anything...but, ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum ...trolls. 3. The AMA process was simple and well organized. You can check out the r/Science AMA Submission...only small downside to the AMA from my end as an organizer/promoter was that I didn’t have the direct link...content on many of the requested topics to the Addgene blog The blog has many other excellent posts on... science talk here). Finally, my main content organizer is the amazing app Evernote. I have a paid account...which gives me all the features I need to save, organize and easily find the resources that I come across...

...reading, and organizing Tips for writing a scientific review article   Write for the Addgene Blog ...also another free citation manager that lets you organize and cite papers. With the Zotero Connector, papers...managers. If you want more tips on finding and organizing papers, check out this related blog post: Early...Resources You Can Grad School podcast episode 2 "Organizing Literature" : I found out about Zotero here Dear...method in grad school Additional resources on the Addgene blog: Tips for writing scientific manuscripts:...

...many organisms. When supplemented with adenine analogs such as 2-fluoroadenine (2-FA), organisms with ...Zurich. He is fascinated by how organisms exquisitely and robustly self-organize at the cellular and multicellular...Resources on Addgene.org Find tools for mammalian RNAi Browse all plasmids for RNAi Check out Addgene's plasmids...Fortunately, researchers studying plants and other organisms lacking this innate immunity need not worry and...work is required, several vectors can be found at Addgene that could serve as an excellent start (#22988,...many tools for shRNA in mammals are available at Addgene. Identifying actively silenced plants Once you’...you’ve introduced your RNAi into your organism, how do you identify the actively silenced ones? Here, I’...

...has taken place in other organisms. In many bacteria, unlike other organisms, CRISPR-induced double stranded...diseases have connections to either specific organisms or organism/gene interactions with the host. This scenario... in bacteria, but have no fear - plasmids from Addgene depositors are making it easier than ever to use...CRISPR-induced double stranded breaks (DSB) are lethal. Addgene depositor Luciano Marraffini’s lab took advantage...CRISPR system in E. coli. The system available from Addgene consists of two plasmids: pCas9: carries Cas9 ...can consider the microbiome as a collection of organisms, their genes, and metabolic processes, occupying...to diseases where the presence of just a single organism, such as Clostridium difficile, could be targeted...

...plasmids with Addgene. These can be used to express genes in a variety of model organisms. Use the links...Lambda) and Higher Organisms (Cambridge, MA: Cell Press). Additional Resources on the Addgene Blog Try Out... attL-containing entry vector.   Pro Tip: Addgene also has ready-made entry clones available for ... will depend on a number of factors, like your organism, desired expression level, and experimental purpose...expression system in just one recombination step. Addgene’s ready-made entry clones can be used with a large...of the Gateway Multisite plasmids available at Addgene, including the Frew Lab Multiple Lentiviral Expression... Genomic DNA and is available for all kind of organisms from mammals to E. coli. Glossary of Gateway cloning...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Set up your own Science by the Pint series and organize evens where scientists can grab a beer and chat...discuss the importance of actionability on the Addgene blog and how SITN short-form blog posts help readers...institutional knowledge and continuity - two lead organizers on each program, one junior and one senior.  ...For more info about starting your own SciComm organization and to learn about some of SITN's other initiatives...Additional Resources on the Addgene Blog Resume Writing for Non-academic Science Careers 10 Steps to a Perfect...

...to ensuring plasmid quality is to stay organized. Here at Addgene, we collect data on every plasmid we ... Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website View our ...the best things about sharing plasmids through Addgene is that we provide an added level of confidence...can take to verify your own plasmids. Staying organized With hundreds of samples coming through our doors...selection markers. You don’t need a fancy database to organize your own plasmid collection, although you will...physical sample and its associated information are at Addgene, we are ready to sequence verify the plasmids. ...plasmid you are working with, but, in most cases at Addgene, we check what we call the “insert,” (the gene ...