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  1. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...meeting.  I experienced this at the ASM General Meeting (now Microbe) in 2013 in Denver, CO. While I had been...and colleagues had moved on as well, so I knew very few people. I didn’t really fit in anywhere and everyone... hashtag I had, however, started a Twitter account, and was following the meeting hashtag. I noticed Holly...Holly Bik live-tweeting about topics I needed to know more about. I caught up with her outside the room...with me talking about the tools I could use, and some experimental hurdles I would encounter. This was invaluable...folks at the meeting. I didn’t know anything about these people at the time, but I met some very accomplished...job, I was willing to introduce myself to someone new and ask for help. That was NOT something I would...

  2. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...listening to Simon & Garfunkel’s “I Am A Rock” on repeat in my bedroom. I realized I could and should be interacting...school’s equivalent of a senior thesis), I knew early on that I wanted to work with a partner. WPI’s emphasis...Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d spent...mutants by myself, I would have slowly gone crazy. As it was, my partner Mike and I split the work, shared... and I am known by some colleagues as the “cross-team guy” at Addgene, an unofficial title that I happily...happily embrace (and admittedly, I may have started that nickname myself). I have my own little Product Management...few in my 20+ year career that I can’t help but feel a bit dubious when I do come across one. Also, remember...

  3. How to Prepare for an Industry Interview

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    Blog Post
    ...my academic jobs, I interviewed with just the PI of the lab and for one position, I just had a phone interview...lasted for 30 minute to an hour. Although I never experienced this, I heard from friends that Scientist positions...story demonstrates I possess.   Bullet points on resumes are like cliff notes, so I use these as a starting...this information. I found it useful to write my list of questions in a notebook that I took with me to ...familiar to you. It was my experience not long ago. I just recently started working in the pharmaceuticals... four more years in academia. During my job hunt, I quickly noticed one of the biggest differences between... interview. I also transitioned between different lab groups without having a formal interview process...

  4. Building Global Connections with the International Mentorship Program USA-EUROPE

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    Blog Post
    ...Europe, something that I would not able to do without a program like this. However, I realized that this ...program was much more than that. I discovered that the challenges that I am facing in my career are common...Oviedo): “I couldn’t be luckier with my mentor, since she does research at UK in the same field that I do: ...will arrive to Spain at one point, since I am not sure that I want to pursue the academic track.” How ...2014-15, University of Valencia): “At the beginning, I registered at IMP to have an opportunity to improve...with mentors who are at a professional level where I would like to be in a few years, helping me to advance...boundaries for both professional and personal development, is essential to excelling in the modern world...

  5. Don’t Wait To Launch Your Health Science Startup

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    Blog Post
    ...facilitate your company’s development  Don’t be afraid to email strangers asking to meet. I have cold emailed ...background and/or connections to even begin this process. I am the cofounder and CEO of miLab, a Boston-based ... talented biomedical engineering team from MIT.   I would like to share my own experiences founding and...and running miLab to describe some key lessons I learned while becoming a health science entrepreneur ... ideas than mediocre people with excellent ideas. I am lucky to have launched miLab with two close friends... 50 company) and is our money guy (CFO).  As CEO, I am responsible for internal operations and building...miLab.  Although many emails resulted in no response, I was still able to get through to a number of experts...

  6. 6 Tips for Grant Writing

    Type
    Blog Post
    ...area, a line about the research I will be doing, and a line about what I hope to discover. This section.... Success breeds success and I have certainly found that the experience I gained in writing small travel... or professor becomes an overnight success. Below I have detailed some tips to improve your grant writing...off, and perhaps the most important bit of advice I can suggest, is to give yourself plenty of time when...when writing a fellowship proposal. Yes, yes, I hear you saying “We know this already!” But, you do need...unlikely to get past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor (your...helpful for them to know what we are doing and why. I always ask kindly friends and family to read my non-expert...

  7. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...universities, and medical schools. I will emphatically deny that I have a magic formula to guarantee a... explain what I was proposing. The result of my test was humbling. My conviction that I had a flair for...anecdotes, and insights. After I wrote the first version of my manual, I added new chapters to incorporate...original blog post below! --Ed.  Eight years ago, I decided to write a "how to" manual on applying for...distill a one-size-fits-all recipe for success. What I can promise is that my suggestions can help you make...think you can accomplish. No matter how many times I say this, candidates regularly try to convince me ...She would then look at me quizzically and ask why I didn't just say that? Bottom line is to appreciate...

  8. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...strong. There are a few women that I see at the gym every day. If I skip, I get a bunch of text messages asking... take larger goals (e.g. Figure out what I want to do after I get my PhD) and break them down into smaller...progress. To illustrate this point, consider that I try to exercise every morning, but the temptation ...on setting SMART goals using a Development Plan template. A Development Plan or mentoring journal will...perhaps, asked someone to support your career development as your mentor. How do you make the most of this... reap rewards in the form of reaching career development goals. Check out Joanne's Reddit AMA Add a ...

  9. Engaging with science and society at pgEd

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    Blog Post
    .... In this post, I hope to tell you a little more about how I ended up at pgEd, what I do there, and how... at pgEd I have always been very interested in the societal aspects of science, even while I was a bench...Personal Genetics Education Project (pgEd), where I currently work, strives to broaden the conversations...communities. As the director of new initiatives at pgEd, I take on a variety of projects in the space of science...studies in molecular biology and epigenetics. So, I sought out a number of different opportunities to ...policy, and science and technology studies (STS). I also worked with a number of organizations in public...freely available online; running professional development (PD) workshops for teachers; organizing Congressional...

  10. Addgene Welcomes John Doench to the Advisory Board

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    Blog Post
    ...explained: “I took a microbiology class my sophomore year and it was the first time that I was in a lab...faculty members and it just blew me away….  I have to come here, I need to spend time here, this is an incredible... school at MIT. Doench excitedly remembers: “When I came to MIT to interview, part of the interview process... his PhD work in Sharp’s lab.  Doench says, “what I loved about [RNAi], ... it was just this complete ...be able to do and to organize it and catalogue it, I mean that’s the critical function really…. Addgene..., you need a tremendous amount of technology development alongside it.” Doench starting working with ... Doench says he enjoys working on technology development under the Platform model at the Broad Institute...

  11. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ... market, I am sure I missed some of your favorite apps. If you know of a software app that I missed, let...Recently I learned that Addgene’s pLKO.1 cloning protocol is viewed around 3,000 times each month. I looked...grosses me out. Maybe it’s just me – I didn’t get a smart phone until after I had finished my PhD and left the...Addgene. I just began uploading our cloning protocols to the site and will keep you updated as I learn to... repository’s most frequently requested plasmids. I wondered how many scientists have used the protocol...used. After learning of it's continued popularity, I decided this protocol would be a worthy first contribution...contribution to the protocols.io community. Did I mention that this protocol is being used around the world...

  12. Negotiating Work and Life: How to Find the Joy

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    Blog Post
    ...some things can’t. I kiss my kids good night every evening I am home no matter how busy I am. Believe it ...lead a charitable cause.  While I can’t make it “all better” for you, I can offer some tips and tactics...penicillin – I am much less worried knowing that she can take care of herself this way (except I am glad she...mental relaxation. And, I’m not sure I should put this in print, but I actually let my daughter skip school...organizations supporting women in science and career development. I try to do a really good job in one area at ...you are saying to yourself, “I’m not married,” or “I don’t have children, so my life will be under control...when they leave home which happens very, very fast! I will never forget the time my eight-year-old daughter...

  13. Editor's Choice, September 2016

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    Blog Post
    ...for Listeria in food products). I picked this post for a few reasons: 1) I think phage are super cool and...for Rare Disease, Running for FOP, Running for AJ I have two picks for the editor’s choice posts this ...could be useful for a wide variety of reasons. 3) I would love to see more posts like this on the Addgene...time we’re raising money for a cause while running. I love running and this event gives me the opportunity...September posts in chronological order here: Four Things I Learned at the 10 Years of iPSCs Symposium Tips for...Picking a Lab and a Project That’s it for now. As I indicated in the opening paragraph, I’m currently ...our attention and has huge potential for the development of therapeutics. It also represents a first for...

  14. Four Factors that Differentiate the Stem Cell Field

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    Blog Post
    ... community with their research, I think I can speak for Addgene when I say that I'm glad we're so fast...suggested that I might be able to attend the 10 Years of iPSCs Symposium earlier this year, I jumped at the...exposed to many scientific communities, but usually I only get to scratch the surface of different fields...conferences. In my nearly 20-year career in science, I have never attended a scientific meeting that hosted... daunting because they have no clear cut answers, I applaud the stem cell community for addressing these...is leading to promising clinical trials for ALS.   I have an uncle with Parkinson's, a half-brother who...questions of ethics can have profound impacts on the development of applications with the potential to save lives...

  15. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post
    ...When I first heard about it, I got excited from a computer scientist perspective. But, the more I thought...space is the Victoria Makerspace in Canada, which I belong to. The team at the Victoria Makerspace is ...thought about it, the more I realized that it cuts across multiple disciplines, which really epitomizes ...in the Victoria area, please stop by. And if not, I suspect you can find a makerspace somewhere near you...counter movement by the public to access basic equipment and to become involved in developing tools and... skills to help do science on the cheap using equipment that would otherwise be discarded. For instance...learned to be resourceful and to repurpose old equipment for new uses. We’ll probably never use the Illumina...

  16. Creating accessible biology activities in schools with BioBits

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    Blog Post
    ... MIT. While I had always enjoyed learning about biology in high school, it wasn’t until I started working...that I learned about from textbooks just made everything click in my head and left me thinking: I wish...in my first molecular biology lab in college that I really fell in love with it. Something about being...wish I had this kind of experience earlier! After all, students build circuits in physics class to learn...Unfortunately, having live cells in a classroom presents equipment needs, such as shakers and incubators, and biosafety...Addgene Blog Find planarian tools for teaching developmental biology Use the pathways over time plasmids ...

  17. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    ...Director of Science Product Management “I knew very early on that I wanted to be an artist/designer. But ..."not a real job," and that I should pursue a real career. In art school, I came across Joseph Campbell...your bliss' mantra and never forgot it. Usually when I see inspiring artists who are thinking about getting...getting into the arts (or any career to be honest), I would tell them to follow your passion. Do what you...Career and skills development can happen anywhere, whether you realize it or not. In the lab, you could...

  18. Healthcare Consulting: A Door to the Business of Life Sciences

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    Blog Post
    ...capabilities. In just two years as a healthcare consultant, I have worked on most of these kinds of projects. Almost... must to be successful in this field. Personally, I have greatly benefited from and appreciated the training...economics, or in some combination of these fields. I got my PhD a couple of years ago in Bioengineering...critical analyses skills were applicable to my cases. I can directly use my understanding of the molecular...pulling your weight with your scientific expertise. Do I have to stick with consulting in the long-term? A ...be a great fit for former healthcare consultants. I have even seen consultants go back to research and...academic lab. How to get your foot in the door When I was first considering preparing for and transitioning...

  19. Finding Your Science Policy Path

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    Blog Post
    ...Administration, I could never get one straight answer about what science policy even is! In this article, I hope .... “But, seriously, what even is science policy?”  I have been asked this many times throughout my short...from academia is the uncertainty of it all. Though I had spent the first few decades of my life following...less than two years of walking away from the bench, I have worked for seven different organizations in a...and diverse workforce. Out of all the career spaces I explored for my post-PhD life, the science policy ...local level. The big secret about science policy that I didn’t learn until arriving here in DC, is that so... to get involved.  Why do you do science policy? I would argue that this is the most important question...

  20. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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    Blog Post
    ...tubes and gels). As a freshman in college I studied development and evolution of histone genes in Strongylocentrotus...tank, and observe the beauty of embryo development in real time. I was already a biology geek, but this ...nailed down my plan to become a research scientist. I recently was invited to attend the first Principal...Enabling Discovery Through Genomic Tools) Program and I met scientists from 21 different labs who clearly ...educational programs for K-12 students. You can see why I was excited to be invited to attend. My job is to ...important models for solutions to the coral reef crisis. I like how they are using protocols.io to create a protocol...studied over time. They recognize individual faces (I guess of other wasps) so can be used to study aspects...

  21. Fostering communication in remote collaborations

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    Blog Post
    ... are collaborating on projects. When I was a graduate student, I led a collaborative project between my...specialized equipment, or ensure that techniques are being performed the same way at different sites. I did a... a lab exchange as part of the collaboration I led. This gave me access to specialized microscopes and...interest in a French clinical cohort, neither of which I would have been able to do from my home lab. Scientific...collaborations are becoming more and more common. I hope these tips are useful whether you’re looking ...

  22. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    Blog Post
    ...people, I quickly realize that a company requires more than just good data to become a product. I spoke ... As a biomedical engineer and scientist, I like to envision that my research will have a direct impact...impact on healthcare and the community. As a result, I have occasionally pondered translating my research...insight into "the random-walk" of a budding startup, I asked Mike to outline 3 pieces that are essential ...Koeris, co-founder and current VP of Business Development & Operations of Sample6, for advice on how to...months in graduate school focusing on concept development and participating in business competitions. When... COO, then moved on to become VP of Business Development & Operations. Starting a biotech company ...

  23. How to Be an Excellent Trainee

    Type
    Blog Post
    ...you can say “I usually need time to gather my thoughts, but always have questions once I do – would it... more generally “How am I doing? Can you tell me what’s going well and what I could improve on?” Having... send me the protocol we’ll be using next week so I can familiarize myself with it.” It won’t be a bother...can you remember everything? Write it all down! And I mean everything! The bare minimum should be taking...have time, would you mind helping me with….” vs. “I need help with…now”. Ask for feedback so you can ...adjustments. This could look like “What are some things I could pay attention to, so my dissections go smoother...Visiting Scientist Edition Mentoring for Scientists: I Have a Mentor, What Now? Will You Be My Mentor? Finding...

  24. AAVs in Retinal Gene Therapy

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    Blog Post
    ...It was by serendipity that I got into the field of gene therapy, more specifically AAV-based retinal ...retinal gene therapy. The year was 2001 and I started a job as a technician in a lab using adeno-associated ...degenerative disease called Retinitis Pigmentosa. I quickly became fascinated by this emerging technology...potential for the treatment of some genetic diseases. I didn’t know it at the time, but gene therapy had fallen...s congenital amaurosis. This is the first of what I, scientists, doctors and patients hope are many more...29868583. PubMed Central PMCID: PMC5960719. 8. Trapani I and Auricchio A., 2018. Seeing the Light after 25 ...being benched due to the high cost of clinical development. As a result, Luk Vandenberghe and colleagues...

  25. Academic vs. Industry Postdocs

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    Blog Post
    ...academic postdoc. Why did I switch? Well, my long-term goal was to enter industry since I was drawn to the idea...grant-writing. The more I talked to people with industry experience, the more I heard that getting into...Desrochers, a postdoctoral scientist at AstraZeneca. Why I chose an industry postdoc I’m currently doing an industry...major life decision, the transition felt scary. While I knew two people from graduate school who were at AstraZeneca...assure me that they were having a positive experience, I was still incredibly nervous about leaving academia...will also have to attend many more meetings. How do I get an industry postdoc? Many companies have industry...AstraZeneca. She has a background in cell and developmental biology and is applying this towards researching...

  26. RUBY-Red Siliques

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    Blog Post
    ...Arabidopsis mutant that can’t set seeds. I study the development of the mutant plants, but the seeds needed...tissue for imaging or collection could contaminate it. I have used it for selecting transformation events and...Zhang, T., Sun, H. et al. Hortic Res. (2020).   I can do this by expressing a gene that rescues the ...RUBY and the construct that rescues the mutation. I will be able to easily choose the green seedlings ...these can be challenging and requires specialized equipment. Reporters for studying gene expression have similar...Willoughby is a graduate student studying plant development and cell-cell communication in the Nimchuk Lab...

  27. An Inside Look into Addgene's Dev Team

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    Blog Post
    ...of the developer and I get to see an approach that is likely different from what I would have done. And... for open science. I hope you enjoyed learning about Addgene’s software development team and how we work...developer. This is another point of collaboration for us. I enjoy doing code reviews because it’s like a little...take turns working on different parts of the code. I think the best way to learn about how any code works...software development happening at Addgene under the hood. A significant portion of our development may not...productivity. Get to know our software development team and the development process at Addgene. Diversity supports...When you think of Addgene, chances are software development is not one of the things that comes to mind. ...

  28. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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    Blog Post
    ...’s. I was facing the prospect of having to do a third postdoc in order to rebuild my resume, so I had ...laboratory lease, etc. I still flip to that page in my lab notebook from time to time. I wrote in large letters...often along the lines of “I hate GMOs, but what you are doing makes sense. I can support that.” That was...in a cutting-edge synthetic biology lab Keira and I worked in the same lab for four years. It was a cutting-edge...cutting-edge synthetic biology lab focused on product development: building a plant that could sense and respond...

  29. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    Blog Post
    ... et al. Although I was able to knock-in a 2xFLAG epitope into my gene of interest, I encountered low efficiency...the model organisms which I use to study gene regulation during developmental processes, CRISPR/Cas9 allows...CRISPR editing in C. elegans improves efficiency  I was fortunate – or unfortunate enough, depending on... alternate approaches. Being a “lazy” geneticist, I developed a co-selection approach relying on repair...courtesy of Jordan Ward, UCSF. In 2015 (Ward, 2015), I demonstrated that selection for repair of a temperature-sensitive...Nitschko V, Obermaier C, Philippou-Massier J, Wieland I, Gaul U, Forstemann K (2014) Efficient chromosomal...selective marker to remain in the edited animal. Development of CRISPR/Cas9 editing strategies in C. elegans...

  30. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    Blog Post
    ...but it works for me. I can often remember which paper I need to open, but when I can’t it’s an easy search...organization system that works for you. I have a folder where I dump all of my papers and they all have... it leads you to the same conclusion. Personally, I like papers that can end with some sort of model (...Scientific Manuscript by Maaike Pols, PhD, a Developmental Editor at F1000Research  ...

  31. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...management. I needed it when balancing a handful of demanding courses, a capstone paper I really wanted...creativity; I have developed these skills through school, jobs, and volunteer work, and I guarantee you...time work, bills, (at times) a social life and rest. I need it just as much in my current role as Customer... and Operations Manager at Addgene. In this role, I balance my daily tasks, meet cross-team project commitments...graduate school. These skills will be useful anywhere I work in the future; they are transferrable across ...interested in nonprofit management and international development.       Additional Resources on the Addgene Blog...

  32. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post
    ... the scientists were waiting. I look forward to sharing new developments on this project and on all the...world. Now that the service is successfully launched, I would like to thank some of the people and organizations...have such an impact on scientific materials sharing. I would also like to recognize a few scientists who ...practices and encouraging us to explore new directions. I would like to thank them for their time, dedication...Addgene Blog and our expanding website resources. I hope this gave you the impression that it took a lot...

  33. Summer SciComm Series: Modes of Communication

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    Blog Post
    ... right way?  First of all, I want to put to rest a common misconception. I find personal bias often unintentionally...how they prefer to consume information. Personally, I would much rather read the instructions than watch...the box. There are so many ways to communicate and I wholeheartedly encourage experimentation. You never...What resources do you have?  No matter the project, I can guarantee you that either resource or time restrictions...re happy with. Know if you’ll need specialized equipment and/or expertise from the start, or if you can... media formats. Cognitively demanding format. Equipment, expense and expertise needed can increase exponentially...

  34. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    Blog Post
    ... funding. I’ve been aware of these problems since I began my own career over 20 years ago, and I’m sure...misrepresent science, or misuse science, and that is what I would call bad science.”  The three chapters in this...describes the detection of gravitational waves--a story I was much less familiar with as a biologist--as an ... to be foremost in readers’ minds. Six months ago I would have nodded my head knowingly and appreciatively...slightly dated by next week.   A stepping stone When I discussed The State of Science with the five Addgene...challenges you face in your career.  Acknowledgements: I would like to thank fellow Addgenies Joanne Kamens...identification of Green Fluorescent Protein (GFP) and its development into an essential tool in biology also falls ...

  35. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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    Blog Post
    ...animals than i-GONAD and is a more technically difficult process. Overall, the main drawback of i-GONAD is...Diagram of ovary and oviduct anatomy with the equipment used for i-GONAD. A micropipette is used to inject ...responsible for Easi-CRISPR have also recently developed i-GONAD as a simple method for delivery of genome editing...germline. In contrast to standard microinjection, i-GONAD is performed directly on pregnant female mice...Ohtsuka et al. compared traditional microinjection and i-GONAD knock-in efficiency using a small ssDNA, and... with a long ssDNA donor was less efficient using i-GONAD (15%) vs. microinjection delivery (25-67%). ....2017.153 PubMed PMID: 29266098 Ohtsuka, Masato, et al. i-GONAD: a robust method for in situ germline genome...

  36. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ...were very anxious at this point. Here's the message I shared with the Addgenies on March 11, 2020 after ...figure out how to deliver when labs finally opened.  I was awed by the teamwork and initiative it took to...and the subsequent fluctuations in distribution. I don’t have space in this blog to call out every Addgene...new projects on track to succeed at the same time. I began approaching science funding organizations to...t raise money except occasionally for capital equipment or resources needed to launch a new service or...

  37. 5 Steps to Writing Grant Proposals for Grad Students

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    Blog Post
    ... week, I felt overwhelmed. Jumbled thoughts such as “I have no idea how to write a grant!”, “I don’t have... mind. Moving forward from that first experience, I learned the details of grant writing, volunteered ...Research strategy The Research Strategy highlights i) the significance and innovation of the project and...chance to delegate funds to supplies, personnel, equipment, travel, etc. As a first time budget writer, you...

  38. 9 tips for a successful postdoctoral experience

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    Blog Post
    ...additional mentors. I found this beneficial for my projects, my training, and when I went on the job market...: What is the most exciting story I can tell with my data?  Can I make better slides that more effectively...signing up for and how can you do it well? Below, I briefly describe some tips for a successful postdoc...unpublished information and reagents. Part of the reason I got my first faculty position was because the search...asked someone at my institute about me, even though I had not asked this person to write a letter of reference... improves your perspective like intense exercise. I highly recommend running or biking or something that...this person cares about your science and your development. Taken together, all of this means that you need...

  39. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...writing habit into beautiful graphs! I like to look at these graphs when I need that extra push to stick with...suggestions on how to make your writing less complex. While I don’t think you should accept all of the suggestions...School podcast episode 2 "Organizing Literature" : I found out about Zotero here Dear Grad Student podcast...use in black. This paper talks more about the development of the De-jargonizer.   Academic Phrasebank  ...

  40. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...creative thinking. I won’t go through all nine here, but I’ll discuss a few that I frequently observe ...optimize virus production with roller skates—though I could get around the lab faster that way— but the ... and prevent groupthink? Here are some ideas that I have found helpful in my team brainstorming sessions...may feel they can share opposing opinions as well. I am sure we have all experienced a meeting where one...protocols and even our Lab Inventory Management system. I hope these tips help you to see that the creative ...form at any time and a member of our Business Development team would review it. This survey generated 26...

  41. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...., Woodard, K., Cepko, C., Guerin, K. I., Rego, M. A., Ersing, I., Bachle, S. M., Kamens, J., & Fan, M...recently released Viral Vectors 101 2nd edition — I was incredibly excited when we picked our viral vector... Viral vectors are, after all, a delivery method. I would be remiss to talk about them without looking...from eBooks to guides to protocols, which was why I was so excited to delve into this data as we updated...Campus on the GENIE website.   Bibliography  Ersing, I., Rego, M., Wang, C., Zhang, Y., DeMaio, K. H., Tillgren...process have shaped and supported our growth and development. We’ve shared what we’ve learned, through manuscripts...

  42. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ... can help you. You Can Talk to Your Editors I wish I had been more cognizant of this during my PhD....the Future of Research (FOR) Boston Symposium 2015. I personally participated as a member of the Career ...ll point out some of the highlights and takeaways I got from the symposium. The goal of the Future of...achieve success in their careers. The take-away message I took from the career panel was that there are many... making it more easily digestible/available. What I viewed as the most important take-away from this session... to trainees navigating the waters of career development. Publishing Panel The Early Career Researchers...

  43. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post
    ...the classroom. I had the opportunity to talk with Provost recently about his development of what is now...planarian regeneration into one of my own courses, I was excited to hear about how he developed his program... like an exciting opportunity for your classroom, I highly recommend you try implementing one. It's likely...learning in a year-long biochemistry laboratory: Part I – guided inquiry – purification and characterization...progenitor cell maintenance in planarians in Developmental Biology (Kimball et al., 2020). Benefits and... pathway to diversify science. Evolution and Development. 24(5):127-130. Kimball, C., Powers, K., Dustin... progenitor cell maintenance in planarians. Developmental Biology. 457:119-127. Knutson, K., Smith, J....

  44. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    .... However, I have a list of what I really need in order to reach my research goals. Would I be able to...include: equipment, reagents, animals, glassware, and even people. When developing a budget, I suggest ...of the major equipment that you use in your current lab first. Generally, major equipment can be defined...requires specialized equipment, try finding institutes that offer shared equipment that you can use at ...defined as equipment with capital costs greater than $5,000 but this can depend on the institution/funding...funding source. Major equipment includes large centrifuges, sequencers and analyzers, and any ultra sensitive...sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying all...

  45. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...thing. Nevertheless, the models are compelling, and I encourage you to hold one. Why do we need physical...physical model. Until then, you can only wonder what I am talking about. How do researchers use physical ...they will almost invariably say something like “Oh,…I didn’t quite appreciate that….” For example, when ...polypeptide emerges from the exit channel.   He then said “I hadn’t realized how perfectly flat the complex is ...an initial solution to this problem with the development of Byron’s Bender – a mechanical device that ...molecular structures for use in professional development programs for bioscience educators. Using these...

  46. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...ion-channels and pumps in developmental biology, regeneration studies, and cancer biology. I was first introduced...introduced to bioelectricity when I attended the Developmental Bioelectricity satellite symposium at the 2019...meeting I had an increased appreciation for the role of ion channels and pumps in developmental biology...2019 Society for Developmental Biology meeting in Boston. The atmosphere was electric! After attending...whole scientific community. Over the years, developmental biologists and cancer researchers are beginning... decision-making in somatic cell networks. “Developmental bioelectricity is an extremely exciting emerging... "The body electric 2.0: recent advances in developmental bioelectricity for regenerative and synthetic...

  47. Reflections and Looking Ahead

    Type
    Blog Post
    ...need to push the boundaries of discovery. As I look ahead, I am excited to continue to grow and innovate...Houston, D. W., Polinski, N. K., Meijers, R., Levey, A. I., Saper, C. B., Errington, T. M., Turn, R. E., Bandrowski...science. Want to learn more about our latest developments? Subscribe to the Addgene newsletter. References...

  48. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...  It was with a sense of relief that I attended the amazing, postdoc-driven Future of Research Conference...supporters from all of the research centers in Boston.  I was relieved because it finally feels to me like the...the Workforce, Metrics and Incentives and Funding.  I can’t wait to see what solutions these talented postdocs...progress reports information on the Individual Development Plans (IDPs) for the graduate students and postdocs...

  49. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ... of tumor antigen. I asked Howarth to tell us more about SpyLigase, its development, and its potential... different approaches to improve CTC capture, but I hope that our antibody tentacles may have an impact...plasmids that may well be of use to others to Addgene. I am a big fan; thanks for the T-shirt! References:...

  50. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ... this event, I gave my own talk on reproducibility at Addgene and here I summarize what I learned both...irreproducibility with some solutions based on what I’ve learned. I hope you can apply some of these solutions to your...align better with an initial hypothesis. To be clear, I don’t think that the majority of researchers do these...whether the ultimate results were positive or negative. I can feel you rolling your eyes, but even if this ideal...plasmid repository 49:45 0 1:18:45 - Reagent Development - Steven C. Almo, Institute for Protein Innovation...
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