We narrowed to 539 results for: try;

...working on, start wherever it makes sense to start and try to be as chronological as possible — if you find ..., be approachable. Engage with your audience and try to learn from them. Science is a collective endeavour...

...yourself enjoying?  To find master’s-level postings, try adding “master’s” to your favorite search term, i.e...how people describe their actual work. To do this, try looking at LinkedIn profiles of people who have masters...switch your field of work, both in academia and in industry. Advancing along a career path in these two fields...fields looks different however. In industry the career ladder is larger and there are more opportunities...Career advancement for PhDs, both in academia and industry, often means moving from the lab to a desk, although...

...experiment will likely depend on: The protein you are trying to purify The expression system If you want the...fusion proteins from being purified by GST (maybe try MBP if you are having solubility issues!). Need a...

...protein will allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get away with generating...downstream of the open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer the more...

...correct plasmid, scientists would spend hours to days trying find their correct clones. Additionally, the presence...cassette within the coding region of the gene they are trying to disrupt or delete, which both inactivates the...

...questions. Talk to as many lab members as possible. Try to get them in a setting out of the lab (coffee, ...Unfortunately, a bad mentor can be bad news for anyone. Try to speak to them more than once to get a full picture...link from 2020) on this topic), for this second entry in the “Mentoring for Scientists Series”, let’s ...

...fluorescence (for some background on fluorescence, try the Introduction to Fluorescence Microscopy video...that work in one context may not work in another. Try to choose primary antibodies that have been validated...time to carefully review your options. If possible, try to select a couple of antibodies that seem promising...see associated with IF are immunocytochemistry (ICC) or immunohistochemistry (IHC). All three of these ...

...example, if your work requires specialized equipment, try finding institutes that offer shared equipment that..., instead of solely discussing numerical values, try to keep conversations focused on research relevant...needs and limitations. Everything is negotiable. Try asking for discounts, collaborations, access to internal...sites (like, indeed.com, or salary.com). Search industry and academic job titles in the regions where the...

...well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique or the Flowtime Technique....these tips are helpful to you no matter what you’re trying to accomplish during this period. ...

...researcher decides on a new project and begins by trying to reproduce someone else’s result. Then, they ...that they thought would be moving forward instead trying just to get going. It’s like being stuck in jail...

...vector is large (>10 kb) or has a low copy number, try following protocols for low copy plasmids. For example...plasmid and the recombined vector backbone, you can try re-streaking for single colonies and testing multiple...

... enough - but this week we’re certainly going to try!      Here’s our Depositor Week schedule:  Monday...

...limitation . Browse Addgene's in-stock AAV preps or try Addgene's Packaged on Request service. Delivery Using...

...from Ma et al., 2018 with permission.   Why try CRISPR-Sirius? With all the tools out there to visualize...visualize genomic loci brightly, give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References...

....  We hope you will give our new search engine a try to find reagents and educational material. Let us...us know what works for you and what doesn’t. Try out the new Addgene search engine! Additional resources...

...Relationships for Success”. Choose 1-2 tactics to try. One of my favorites is making the pledge to have... being an educated mentee. Other opportunities: Try virtual mentoring at Mentornet.net. This is an online...seeing a lot of success at universities around the country with groups who are piloting this. Watch for my...

...occasional errors. That can be advantageous if you are trying to knock out a gene. One can also exploit homology-directed...induced pluripotent stem (iPS) cells from patients and try to compare those lines, they may all have the mutations...

...molecular cloning. Depending on whether you are trying to overexpress or knockdown a gene, you might need...not always clear which side he roots for. You can try to convince him to use Twitter @jonchowphd but he...

...RNA structure and the site in the genome you are trying to target play a large role in Cas9 activity and... CRISPR mutant of interest, give these methods a try.  References Højland Knudsen C, Ásgrímsdóttir...homology-directed repair. Journal of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074/jbc.m117.777722...

...how the scavenger hunt works works: You will be trying to find pictures of Addgene pets with Addgene-branded...

...that blue and violet light can penetrate. How can I try using photoswitchable kinases in my experiments? ...into loops of a protein of interest. If you plan to try this, it’s important to check that the function of...

...send an unpersonalized, generic invitation. Don’t try to connect from the LinkedIn phone app either as ...suit, but this is not Facebook so no pets or kids. Try looking a bit over your shoulder for the picture....

...during protein folding, in which case you should try tagging the opposite end.  If neither placement works...protein of interest is expressed at a very low level, try a longer histidine tag to increase capture. Longer...

...nitrocellulose, you may decide you have nothing to lose by trying to strip and reprobe. In this case, we recommend...procedure, perhaps using longer incubation times, or try the stringent solution if you started with the mild...

... you. When embarking on a new literature search, try to find a recent review article to give you an overview...you are looking for a particular protocol or are trying to replicate an experiment, the methods section... you to our guest blogger! Johnna Roose is a biochemistry instructor in the Department of Biological Sciences...photosynthesis and writes about autotrophs and biochemistry on her blog. Follow her on Twitter @JLRoose....

...gloves can sometimes be annoying. To make it easier, try this quick trick. Just flip, and press! Lab tip...

...summarize, but we wanted to mark the occasion by trying.  When we started in 2004, we were the plasmid...

...not doing yourself or your science any favors by trying to go it alone. The help of fellow lab members .... If I invite someone to be on a project team, I try to make it very clear why that person is there. If...thinking “What’s my angle on attacking this problem?”, try thinking “Who can I assemble to attack this problem...

... get a lot of office politics (many individuals trying to divert more resources towards their projects...modern genetics from the perspective of somebody trying to get hands-on lab experience is that the technology... may seem quite unusual but it has allowed me to try out working in many different sectors and to discover...applied for a role in Industry. I wanted to get "a real job" and roles in Industry do tend to pay better... (GLP) (important buzzwords in industry) as well as skills in industry positions. There were, however... a little different. Industry and academic jobs do exist, but working in industry just wasn't for me, ...months experience working in a developing tropical country. This is why I set up a placement working in a ...

...currently available, you now face the challenge of trying to figure out what system might work best for your...streamlined approach, you might want to give Gateway a try. This method, developed by Invitrogen and based on...

...system that is the best fit for the experiment, trying to produce virus of a usable titer, or fine-tuning...beginning an experiment, it is recommended that you try a few different ratios of transfer, envelope, and...

...Experiments tend to be equally complex with users trying to probe multiple targets in parallel. In these...cross-reactivity. To further limit species cross-reactivity, try to find secondary antibodies that are all derived...Mouse Tissue Sections. In Journal of Histochemistry & Cytochemistry (Vol. 46, Issue 8, pp. 977–983). ...

...first (and second) you won’t succeed, but you must try, try again. Now, this does not mean that you should...a different university, perhaps in a different country, choose where you go carefully. Funding agencies...

...started: Determine what type of outcome you are trying to achieve: Do you want to permanently knock out...to activate or repress gene expression? Are you trying to create a single point mutation? Do you want ...

...gondii genes.  2) What genetic modification are you trying to make? Addgene carries CRISPR libraries for gene...activation, repression, and barcoding.  3) Are you trying to target every gene in the genome, or a specific...

...and to get their answers to the questions. Don't try to get all panelists on the phone at once – you won't...knows some of the people in the audience. I always try to meet audience members before a panel discussion...

...to verify plasmids or validating your new clones, try these tips to make the most of your time and sequence... a number of sequence alignment tools available. Try these resources for lists of alternatives to BLAST...databases available are unlikely to have an exact entry for your favorite gene fused to an epitope tag or...

...Meet People at a Scientific Conference New to SfN? Try A Novice's Perspective on Neuroscience - SfN17!  ...

... maybe even picked up a new technique or tool to try in your own work!) If you've got a research story...

...glycerol stock before you begin your plasmid mini-prep. Try not to freeze/thaw your glycerol stock too many times...

... that you cannot perform your duties. Be sure to try out different sizes to know your fit. It is best ...

...much easier to go where your audience is than to try to convince your audience to come to you (a TikTok... project like a hot potato or burn yourself out trying to slog through unappealing work in your precious...used in all countries; not all areas of any given country have equal access to high-speed internet. Before... Written works Low Flexible. Low barrier to entry. Ample distribution channels both digitally and ...discouraging to build audience. Need space & higher entry barrier for audience versus passive digital consumption...combine multiple types of media. High barrier to entry (people have to enter a building or exhibit). No...video. Easy to target audiences. Low barrier to entry for both audience and communicators. Well-developed...

...confocal microscope. You’re trying to get better images for a paper and she’s trying to get some data for an...

...and doing everything, it can be annoying when we try to use them in an experiment and they don't do anything...during replication. To avoid genomic rearrangements, try amplifying the viral vector with bacteria designed...adsorption on target cell membranes." Biophysical chemistry 97.2 (2002): 159-172. PubMed PMID: 12050007. 7...

...molecular biology tool kit. Consider giving it a try next time you’re screening for positive clones! ...the Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional...

... writing a review, or if you’re currently stuck trying to write one, hopefully this post will help you... or recent review exists. Once you pick a topic, try to be specific about exactly what aspect of the field...

...Recombines with the entry clone to generate an expression clone   Ready to try out Gateway cloning?...Press). Additional Resources on the Addgene Blog Try Out FastCloning Learn About Methylation and Restriction...to generate entry clones Entry vector attL sites for recombination Used to generate entry clones by TOPO...1: Generate an Entry Clone There are a few different ways to generate our desired entry clone - human ... an attL-containing entry vector.   Figure 4: Method B to create an entry clone: TOPO cloning ... an attL-containing entry vector.   Figure 5: Method C to create an entry clone: Restriction cloning...an attL-containing entry vector.   Pro Tip: Addgene also has ready-made entry clones available for...

...less resources and are particularly useful when trying to screen a limited number of cells like primary... plays a significant role in library efficiency. Try the Dolcetto library! Now also available as a lentiviral...

... and should be designed accordingly. If you are trying activate a gene, you should design a control that...that shows maximal activation. Likewise if you are trying to repress a gene, your control might be a system...

...protocols, and, of course, plasmids. Today we’re trying a new way to present plasmid info with a new segment...