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  1. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ... boss. But it does not mean shouting orders and trying to get everything done your way. According to Merriam-Webster... direct with a degree of skill, To work upon or try to alter for a purpose and To direct the professional... you will take responsibility (within reason!).  Try to buffer them from unnecessary pressure “coming ... What Makes a Good Mentor? Tips for Working in Industry and Success in Management Resources at Addgene.org...

  2. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...another industry-geared option, try the Biospace career events page. Biospace holds career events all over...necessarily need to be someone a lot older than you are; try using ResearchGate to see where other graduate students...and post-docs from your institution have gone, and try sending them a professional email asking for information...employment within industry or academia; although the ACS career fair is more industry-focused. For another...over the country; sometimes several per month, giving you a plethora of opportunities to explore! Again...

  3. The Strength of Story Telling

    Type
    Blog Post
    ...working on, start wherever it makes sense to start and try to be as chronological as possible — if you find ..., be approachable. Engage with your audience and try to learn from them. Science is a collective endeavour...

  4. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...experiment will likely depend on: The protein you are trying to purify The expression system If you want the...fusion proteins from being purified by GST (maybe try MBP if you are having solubility issues!). Need a...

  5. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ...yourself enjoying?  To find master’s-level postings, try adding “master’s” to your favorite search term, i.e...how people describe their actual work. To do this, try looking at LinkedIn profiles of people who have masters...switch your field of work, both in academia and in industry. Advancing along a career path in these two fields...fields looks different however. In industry the career ladder is larger and there are more opportunities...Career advancement for PhDs, both in academia and industry, often means moving from the lab to a desk, although...

  6. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...protein will allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get away with generating...downstream of the open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer the more...

  7. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...correct plasmid, scientists would spend hours to days trying find their correct clones. Additionally, the presence...cassette within the coding region of the gene they are trying to disrupt or delete, which both inactivates the...

  8. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...questions. Talk to as many lab members as possible. Try to get them in a setting out of the lab (coffee, ...Unfortunately, a bad mentor can be bad news for anyone. Try to speak to them more than once to get a full picture...link from 2020) on this topic), for this second entry in the “Mentoring for Scientists Series”, let’s ...

  9. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...fluorescence (for some background on fluorescence, try the Introduction to Fluorescence Microscopy video...that work in one context may not work in another. Try to choose primary antibodies that have been validated...time to carefully review your options. If possible, try to select a couple of antibodies that seem promising...see associated with IF are immunocytochemistry (ICC) or immunohistochemistry (IHC). All three of these ...

  10. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...example, if your work requires specialized equipment, try finding institutes that offer shared equipment that..., instead of solely discussing numerical values, try to keep conversations focused on research relevant...needs and limitations. Everything is negotiable. Try asking for discounts, collaborations, access to internal...sites (like, indeed.com, or salary.com). Search industry and academic job titles in the regions where the...

  11. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique or the Flowtime Technique....these tips are helpful to you no matter what you’re trying to accomplish during this period. ...

  13. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...researcher decides on a new project and begins by trying to reproduce someone else’s result. Then, they ...that they thought would be moving forward instead trying just to get going. It’s like being stuck in jail...

  14. 2022 Depositor Week

    Type
    Blog Post
    ... enough - but this week we’re certainly going to try!      Here’s our Depositor Week schedule:  Monday...

  15. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ....  We hope you will give our new search engine a try to find reagents and educational material. Let us...us know what works for you and what doesn’t. Try out the new Addgene search engine! Additional resources...

  16. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...from Ma et al., 2018 with permission.   Why try CRISPR-Sirius? With all the tools out there to visualize...visualize genomic loci brightly, give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References...

  17. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ...Relationships for Success”. Choose 1-2 tactics to try. One of my favorites is making the pledge to have... being an educated mentee. Other opportunities: Try virtual mentoring at Mentornet.net. This is an online...seeing a lot of success at universities around the country with groups who are piloting this. Watch for my...

  18. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...occasional errors. That can be advantageous if you are trying to knock out a gene. One can also exploit homology-directed...induced pluripotent stem (iPS) cells from patients and try to compare those lines, they may all have the mutations...

  19. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...RNA structure and the site in the genome you are trying to target play a large role in Cas9 activity and... CRISPR mutant of interest, give these methods a try.  References Højland Knudsen C, Ásgrímsdóttir...homology-directed repair. Journal of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074/jbc.m117.777722...

  22. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...send an unpersonalized, generic invitation. Don’t try to connect from the LinkedIn phone app either as ...suit, but this is not Facebook so no pets or kids. Try looking a bit over your shoulder for the picture....

  23. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...nitrocellulose, you may decide you have nothing to lose by trying to strip and reprobe. In this case, we recommend...procedure, perhaps using longer incubation times, or try the stringent solution if you started with the mild...

  24. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...that blue and violet light can penetrate. How can I try using photoswitchable kinases in my experiments? ...into loops of a protein of interest. If you plan to try this, it’s important to check that the function of...

  25. To Each HIS Own

    Type
    Blog Post
    ...during protein folding, in which case you should try tagging the opposite end.  If neither placement works...protein of interest is expressed at a very low level, try a longer histidine tag to increase capture. Longer...

  26. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ... you. When embarking on a new literature search, try to find a recent review article to give you an overview...you are looking for a particular protocol or are trying to replicate an experiment, the methods section... you to our guest blogger! Johnna Roose is a biochemistry instructor in the Department of Biological Sciences...photosynthesis and writes about autotrophs and biochemistry on her blog. Follow her on Twitter @JLRoose....

  27. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...gloves can sometimes be annoying. To make it easier, try this quick trick. Just flip, and press! Lab tip...

  29. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...not doing yourself or your science any favors by trying to go it alone. The help of fellow lab members .... If I invite someone to be on a project team, I try to make it very clear why that person is there. If...thinking “What’s my angle on attacking this problem?”, try thinking “Who can I assemble to attack this problem...

  30. Finding Your Perfect Job After University

    Type
    Blog Post
    ... get a lot of office politics (many individuals trying to divert more resources towards their projects...modern genetics from the perspective of somebody trying to get hands-on lab experience is that the technology... may seem quite unusual but it has allowed me to try out working in many different sectors and to discover...applied for a role in Industry. I wanted to get "a real job" and roles in Industry do tend to pay better... (GLP) (important buzzwords in industry) as well as skills in industry positions. There were, however... a little different. Industry and academic jobs do exist, but working in industry just wasn't for me, ...months experience working in a developing tropical country. This is why I set up a placement working in a ...

  32. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...system that is the best fit for the experiment, trying to produce virus of a usable titer, or fine-tuning...beginning an experiment, it is recommended that you try a few different ratios of transfer, envelope, and...

  33. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...Experiments tend to be equally complex with users trying to probe multiple targets in parallel. In these...cross-reactivity. To further limit species cross-reactivity, try to find secondary antibodies that are all derived...Mouse Tissue Sections. In Journal of Histochemistry & Cytochemistry (Vol. 46, Issue 8, pp. 977–983). ...

  34. 6 Tips for Grant Writing

    Type
    Blog Post
    ...first (and second) you won’t succeed, but you must try, try again. Now, this does not mean that you should...a different university, perhaps in a different country, choose where you go carefully. Funding agencies...

  36. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...gondii genes.  2) What genetic modification are you trying to make? Addgene carries CRISPR libraries for gene...activation, repression, and barcoding.  3) Are you trying to target every gene in the genome, or a specific...

  37. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...and to get their answers to the questions. Don't try to get all panelists on the phone at once – you won't...knows some of the people in the audience. I always try to meet audience members before a panel discussion...

  38. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...to verify plasmids or validating your new clones, try these tips to make the most of your time and sequence... a number of sequence alignment tools available. Try these resources for lists of alternatives to BLAST...databases available are unlikely to have an exact entry for your favorite gene fused to an epitope tag or...

  39. Addgene at SfN23!

    Type
    Blog Post
    ...Meet People at a Scientific Conference New to SfN? Try A Novice's Perspective on Neuroscience - SfN17!  ...

  43. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...much easier to go where your audience is than to try to convince your audience to come to you (a TikTok... project like a hot potato or burn yourself out trying to slog through unappealing work in your precious...used in all countries; not all areas of any given country have equal access to high-speed internet. Before... Written works Low Flexible. Low barrier to entry. Ample distribution channels both digitally and ...discouraging to build audience. Need space & higher entry barrier for audience versus passive digital consumption...combine multiple types of media. High barrier to entry (people have to enter a building or exhibit). No...video. Easy to target audiences. Low barrier to entry for both audience and communicators. Well-developed...

  44. Transferable Skills: Negotiation

    Type
    Blog Post
    ...confocal microscope. You’re trying to get better images for a paper and she’s trying to get some data for an...

  45. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...and doing everything, it can be annoying when we try to use them in an experiment and they don't do anything...during replication. To avoid genomic rearrangements, try amplifying the viral vector with bacteria designed...adsorption on target cell membranes." Biophysical chemistry 97.2 (2002): 159-172. PubMed PMID: 12050007. 7...

  46. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...molecular biology tool kit. Consider giving it a try next time you’re screening for positive clones! ...the Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional...

  47. How to Write a Scientific Review Article

    Type
    Blog Post
    ... writing a review, or if you’re currently stuck trying to write one, hopefully this post will help you... or recent review exists. Once you pick a topic, try to be specific about exactly what aspect of the field...

  48. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...Recombines with the entry clone to generate an expression clone   Ready to try out Gateway cloning?...Press). Additional Resources on the Addgene Blog Try Out FastCloning Learn About Methylation and Restriction...to generate entry clones Entry vector attL sites for recombination Used to generate entry clones by TOPO...1: Generate an Entry Clone There are a few different ways to generate our desired entry clone - human ... an attL-containing entry vector.   Figure 4: Method B to create an entry clone: TOPO cloning ... an attL-containing entry vector.   Figure 5: Method C to create an entry clone: Restriction cloning...an attL-containing entry vector.   Pro Tip: Addgene also has ready-made entry clones available for...

  50. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ... and should be designed accordingly. If you are trying activate a gene, you should design a control that...that shows maximal activation. Likewise if you are trying to repress a gene, your control might be a system...
Showing: 51 - 100 of 535 results