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Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- or dox-inducible systems.

Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit.

Find plasmids and resources for FRET experiments to measure protein interactions or conformation changes.

From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently.

Plamids for Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function in zebrafish, plants, and human cells

Learn about how CRISPR systems are classified. Addgene's guide to typing CRISPR.

A selection of Addgene's empty vector backbones curated by species, epitope tags/fusion proteins, function, selectable markers, and more.

A review of K. phaffii as a model organism and its relevant applications for biotech to basic research.

Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more.

Learn about how Cascade-Cas3 can be used as a CRISPR tool to degrade large fragments of DNA.

Data-driven design of Golden Gate Assembly overhangs greatly increases the number of fragments that can be used in cloning.

This post discusses the core replication and resistance features of yeast vectors and explains how these plasmids differ from their bacterial counterparts.

This post discusses the applications, advantages, and caveats to using hybrid guide RNAs, in which DNA bases are substituted for RNA bases.

Ready-to-use AAV available from Addgene's viral service encoding tools for monosynpatic retrograde neuronal tracing, to be used with modified rabies virus.

This post describes evoCAST, a new CRISPR-associated transposase tools optimized for genetic editing in human cells.

Curious about how, exactly, Cas9 does what it does? Dive into the structural biology of Cas9 for an answer!

Comparing and contrasting Cas9 to the other genome editing-capable Cas family proteins.

Learn how you can use plasmids to teach students about functional genomics with your own "Pathways Over Time" project.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

Adeno-associated virus (AAV) vector serotypes can help you target specific cell types through their tissue tropism.