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Showing: 21 - 40 of 109 results

  2. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...Volume 42, Issue 22, Page e168. https://doi.org/10.1093/nar/gku936 Eva K. Brinkman, Arne N. Kousholt, Tim...Volume 46, Issue 10, Page e58. https://doi.org/10.1093/nar/gky164 Cancellieri S, Canver MC, Bombieri N...) Bioinformatics 36:2001–2008. https://doi.org/10.1093/bioinformatics/btz867 Haeussler M, Schönig K, Eckert...

  3. Typing CRISPR Systems

    Type
    Blog Post
    ...Acids Research, 42(10), 6091–6105. https://doi.org/10.1093/nar/gku241 Cong, L., Ran, F. A., Cox, D., Lin, ...Sciences, 374(1772), 20180087. https://doi.org/10.1098/rstb.2018.0087 Koonin, E. V., Makarova, K. S., ...Acids Research, 48(4), 2000–2012. https://doi.org/10.1093/nar/gkz1197 Samai, P., Pyenson, N., Jiang, W., ...

  5. Deep Dive: qPCR

    Type
    Blog Post
    ...1 April 2011, Pages 1019–1020, https://doi.org/10.1093/bioinformatics/btr065Freeman WM; Walker SJ; Vrana...Aug;29(2):332-7. doi: 10.2144/00292rv02. PMID: 10948434.Taylor SC; Laperriere G;  Germain H. Droplet Digital...October 2000, Pages 3752–3761, https://doi.org/10.1093/nar/28.19.3752Wong M; Medrano J. Real-time PCR ...

  6. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ... Acids Research 41:7429–7437 . https://doi.org/10.1093/nar/gkt520 Citorik RJ, Mimee M, Lu TK (2014) Sequence-specific...Biochimica et Biophysica Sinica. https://doi.org/10.1093/abbs/gmab036 Liu Y, Wan X, Wang B (2019) Engineered...Nucleic Acids Res 44:e143–e143 . https://doi.org/10.1093/nar/gkw625 Resources on the Addgene Blog Check...

  8. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 10.1073/pnas.1216880109 Resources on Addgene.org CRISPR...

  9. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...Acids Research, 50(2), 617–634. https://doi.org/10.1093/nar/gkab1256 Lee, Y., Kim, M., Han, J., Yeom, K...and regulation. Biophysical Reviews, 13(6), 1081–1090. https://doi.org/10.1007/s12551-021-00902-w Additional...

  10. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ- JM109   General cloning and plasmid maintenance, blue/...hsdR514 endA recA1 uidA(∆MluI)::pir-116  Stbl2   JM109-derived. For cloning into and storage of lentiviral...

  11. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...Nucleic Acids Res 44:e141–e141 . https://doi.org/10.1093/nar/gkw583 Rees HA, Liu DR (2018) Base editing:... Acids Research 41:9049–9061 . https://doi.org/10.1093/nar/gkt555 Resources at the Addgene Blog Listen...

  12. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...Nucleic Acids Research 47:e23–e23. https://doi.org/10.1093/nar/gky1286 Hilton IB, D’Ippolito AM, Vockley CM... Acids Research 44:5615–5628 . https://doi.org/10.1093/nar/gkw159 Williams RM, Senanayake U, Artibani ...

  15. Reflections and Looking Ahead

    Type
    Blog Post
    ...Nature Protocols. https://doi.org/10.1038/s41596-024-01095-8 Kahn, R. A., Virk, H., Laflamme, C., Houston, ...

  18. Hot Plasmids - October 2022

    Type
    Blog Post
    ...–974 (2022). https://doi.org/10.1038/s41593-022-01094-6. (This work was also shared as a preprint: https...
Showing: 21 - 40 of 109 results