Viral Vectors 101: Pseudotyping
Type
Blog Post
...Human Molecular Genetics 10:2109–2121 . https://doi.org/10.1093/hmg/10.19.2109
Sandrin V, Boson B, Salmon...
We narrowed to 108 results for: 109
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CRISPR 101: Validating Your Genome Edit
TypeBlog Post...Volume 42, Issue 22, Page e168. https://doi.org/10.1093/nar/gku936 Eva K. Brinkman, Arne N. Kousholt, Tim...Volume 46, Issue 10, Page e58. https://doi.org/10.1093/nar/gky164 Cancellieri S, Canver MC, Bombieri N...) Bioinformatics 36:2001–2008. https://doi.org/10.1093/bioinformatics/btz867 Haeussler M, Schönig K, Eckert... -
Affinity Purification of Recombinant Antibodies with Protein A or Protein G
TypeProtocol... VWR 89126-724 Filtering system 0.2 µm PES, VWR 431098 Sodium phosphate monobasic monohydrate, Millipore... -
Typing CRISPR Systems
TypeBlog Post...Acids Research, 42(10), 6091–6105. https://doi.org/10.1093/nar/gku241 Cong, L., Ran, F. A., Cox, D., Lin, ...Sciences, 374(1772), 20180087. https://doi.org/10.1098/rstb.2018.0087 Koonin, E. V., Makarova, K. S., ...Acids Research, 48(4), 2000–2012. https://doi.org/10.1093/nar/gkz1197 Samai, P., Pyenson, N., Jiang, W., ... -
Deep Dive: qPCR
TypeBlog Post...1 April 2011, Pages 1019–1020, https://doi.org/10.1093/bioinformatics/btr065Freeman WM; Walker SJ; Vrana...Aug;29(2):332-7. doi: 10.2144/00292rv02. PMID: 10948434.Taylor SC; Laperriere G; Germain H. Droplet Digital...October 2000, Pages 3752–3761, https://doi.org/10.1093/nar/28.19.3752Wong M; Medrano J. Real-time PCR ... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post... Acids Research 41:7429–7437 . https://doi.org/10.1093/nar/gkt520 Citorik RJ, Mimee M, Lu TK (2014) Sequence-specific...Biochimica et Biophysica Sinica. https://doi.org/10.1093/abbs/gmab036 Liu Y, Wan X, Wang B (2019) Engineered...Nucleic Acids Res 44:e143–e143 . https://doi.org/10.1093/nar/gkw625 Resources on the Addgene Blog Check... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog Post... (EMBC), 4322–4325. https://doi.org/10.1109/EMBC44109.2020.9175600 Park, S., Loke, G., Fink, Y., & Anikeeva... -
Plasmids 101: Degron Tags
TypeBlog Post...Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: https://doi.org/10.1073/pnas.1216880109 Resources on Addgene.org... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post...Acids Research, 50(2), 617–634. https://doi.org/10.1093/nar/gkab1256 Lee, Y., Kim, M., Han, J., Yeom, K...and regulation. Biophysical Reviews, 13(6), 1081–1090. https://doi.org/10.1007/s12551-021-00902-w Additional... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post...lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ- JM109 General cloning and plasmid maintenance, blue/...hsdR514 endA recA1 uidA(∆MluI)::pir-116 Stbl2 JM109-derived. For cloning into and storage of lentiviral... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...Nucleic Acids Research 47:e23–e23. https://doi.org/10.1093/nar/gky1286 Hilton IB, D’Ippolito AM, Vockley CM... Acids Research 44:5615–5628 . https://doi.org/10.1093/nar/gkw159 Williams RM, Senanayake U, Artibani ... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog Post... a thermostable DNA ligase-encoding gene." Gene 109.1 (1991): 1-11. PubMed PMID: 1756968. 7. Lauer, G.... research 10.5 (1982): 1425-1437. PubMed PMID: 7041091. PubMed Central PMCID: PMC320540. 10. Goffin, Colette... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...Nucleic Acids Research 42:e89–e89 . https://doi.org/10.1093/nar/gku289 Chen C, Fenk LA, de Bono M (2013) Efficient... Acids Research 41:e193–e193 . https://doi.org/10.1093/nar/gkt805 Dickinson DJ, Pani AM, Heppert JK, Higgins... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog Post...Nucleic Acids Res 44:e141–e141 . https://doi.org/10.1093/nar/gkw583 Rees HA, Liu DR (2018) Base editing:... Acids Research 41:9049–9061 . https://doi.org/10.1093/nar/gkt555 Resources at the Addgene Blog Listen... -
Reflections and Looking Ahead
TypeBlog Post...Nature Protocols. https://doi.org/10.1038/s41596-024-01095-8 Kahn, R. A., Virk, H., Laflamme, C., Houston, ... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...JJ. Nucleic Acids Res. 2007;35(5):1402-10. doi:10.1093/nar/gkl1108. Epub 2007 Feb 6. PubMed. ... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post...Zakeri et al (Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24. PubMed) ... -
The Pleiades Promoter Project
TypeCollection... Ple108 HBEGF pEMS1253 EGFP/NLS Ple109 HBEGF pEMS1254 EGFP/NLS Ple109 HBEGF pEMS1580 intron-lacZ/NLS Ple110...NLS Ple167 POGZ pEMS1091 EGFP/cre/NLS Ple167 POGZ pEMS1427 EGFP/NLS Ple168 POGZ pEMS1092 EGFP/cre/NLS Ple168...Ple200 SLC6A5 pEMS1096 EGFP/cre/NLS Ple200 SLC6A5 pEMS1407 EGFP/NLS Ple201 SLC6A5 pEMS1097 EGFP/cre/NLS...NLS Ple173 RAMP3 pEMS1412 EGFP/NLS Ple174 RAMP3 pEMS1109 EGFP/cre/NLS Ple174 RAMP3 pEMS1413 EGFP/NLS Ple175...Ple177 RGS16 pEMS1649 intron-lacZ/NLS Ple178 RGS16 pEMS1090 EGFP/cre/NLS Ple178 RGS16 pEMS1366 EGFP/NLS Ple178... SLC6A5 pEMS1673 intron-lacZ/NLS Ple202 SLC6A5 pEMS1098 EGFP/cre/NLS Ple202 SLC6A5 pEMS1409 EGFP/NLS Ple203...NLS Ple230 THY1 pEMS1422 EGFP/NLS Ple231 TNNT1 pEMS1099 EGFP/cre/NLS Ple231 TNNT1 pEMS1435 EGFP/NLS Ple232... -
Hot Plasmids - October 2022
TypeBlog Post...–974 (2022). https://doi.org/10.1038/s41593-022-01094-6. (This work was also shared as a preprint: https... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...developed (mNeptune684 with C65S, T107K, A108V, T109K, H161Y, P163T, I175Q, C176T and N177F mutations)...