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We narrowed to 89 results for: 5-Oct

Showing: 21 - 40 of 89 results

  1. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Mechanisms, detection, and control. Int J Pharm. 2018 Oct 25;550(1-2):251-268. doi: 10.1016/j.ijpharm.2018.08.043...Edelhoch method. Int J Biol Macromol. 2015;77:260-5. doi: 10.1016/j.ijbiomac.2015.03.027. Epub 2015 Mar... in Colloid & Interface Science, Volume 19, Issue 5, 2014, Pages 438-449, ISSN 1359-0294, https://doi....

  2. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...versus analog real-time PCR. Nat Methods. 2013 Oct;10(10):1003-5. PubMed PMID: 22035192. PubMed Central PMCID...PMID: 20486768. PubMed Central PMCID: PMC2957240. 5.Taylor, Sean C., Genevieve Laperriere, and Hugo Germain...

  5. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... Technologies. Cold Spring Harbor Laboratory, 27 Oct. 2023. Crossref, https://doi.org/10.1016/j.xgen.2024.100519...Coli Synthetic Biology.” ACS Synthetic Biology, vol. 5, no. 10, American Chemical Society (ACS), 2 May 2016...Traditional Biology.” ACS Synthetic Biology, vol. 5, no. 1, American Chemical Society (ACS), 4 Nov. 2015....” PLOS ONE, edited by Alvaro Galli, vol. 13, no. 5, Public Library of Science (PLoS), 30 May 2018, p.... vol. 8, no. 11, American Chemical Society (ACS), 5 Nov. 2019, pp. 2593–606. Crossref, https://doi.org...

  6. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...Last updated on Oct 1, 2020 by Aliyah Weinstein. This post was contributed by guest bloggers, Wenning... best case, we saw that 2 out of 3 mice carried a 5 kb insertion in the ROSA locus as assessed by Southern... mouse gene with its human ortholog (greater than 5 kb), it remains to be seen whether CRISPR is as robust...

  7. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...ACS Chem Biol 2014 May 16;9(5):1104-12. Heinemann et al., FEBS Lett 2012 Oct 19;586(20):3716-22. Park...transgenesis constructs. Kirchmaier et al., PLOS One 2013 Oct 7;8(10):e76117. Irreversible peptide-peptide ligation...creating a single transcriptional unit (Ex: a promoter, 5’UTR, coding region, and terminator). Next, up to six...e16765. Engler et al., ACS Synth Biol 2014. Feb 5 (Web); DOI: 10.1021/sb4001504. For further reading...Stigleoclonium helveticum (ShChR), Chlamydomonas noctigama (CnChR1), Chloromonas subdivisa (CsChR), Chloromonas...

  8. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...773–779 . https://doi.org/10.1038/s41477-020-0704-5 Santana I, Wu H, Hu P, Giraldo JP (2020) Targeted ...

  9. Immunology Research Plasmids and Resources

    Type
    Collection
    ... HTR3A 5-hydroxytryptamine (serotonin) receptor 3A 5-HT-3, 5-HT3A, 5-HT3R, 5HT3R, HTR3 HTR3B 5-hydroxytryptamine...heavy diversity 5-24 (non-functional) IGHD524 IGHD5-5 immunoglobulin heavy diversity 5-5 DK4, IGHD55 IGHD6...:14099, K5-5, MGC88293 CCR5 chemokine (C-C motif) receptor 5 CC-CKR-5, CCCKR5, CD195, CKR-5, CKR5, CMKBR5...receptor 3B 5-HT3B HTR3C 5-hydroxytryptamine (serotonin) receptor 3, family member C - HTR3D 5-hydroxytryptamine... interleukin 5 (colony-stimulating factor, eosinophil) EDF, IL-5, TRF IL5RA interleukin 5 receptor, alpha...

  10. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...PAmCherry3 570 596 5 6.2 25 min Monomer pPAmCherry3-C1 - Mammalian Expression PAmKate 568 628 5 5.6 19 min Monomer...2014 Vol. 11 No. 5, pp.572-8 Yu et al. : Nature Communications, May 2014 Vol. 15 No. 5, p. 3626 Do you ...pSBFP2-C1 - Mammalian Expression Azurite 383 450 14 5 Prone to dimerization pLV-Azurite - Mammalian Lentiviral...pB18cmBFPAzurite - Bacterial Expression mAzurite 383 450 14 5 Monomer (A206K) mAzurite-N1 - Mammalian Expression...pmTurquoise2-N1 - Mammalian Expression CyPet 435 477 18 5 Monomer pCEP4CyPet-MAMM - Mammalian Expression pCyPet-C1...Structure Plasmids mKusabira-Orange (mKO) 548 559 31 5 4.5 hr Monomer pQC mKorange IX - Mammalian Expression...mRaspberry-N1 - Mammalian Expression TagRFP675 598 675 4 5 Prone to dimerization pTagRFP675-N1 - Mammalian Expression...

  11. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...Hair Cell-Like Cells. Stem Cells Transl Med, 2016. 5(5): p. 561-71. PubMed PMID: 27013738. PubMed Central...include retrovirus [2], lentivirus [4], adenovirus [5], Sendai virus [6], plasmid electroporation (episomal...in vitro where treatment of mouse fibroblasts with 5-azacytidine led to their conversion into myoblasts...human fibroblasts by defined factors. Cell, 2007. 131(5): p. 861-72. PubMed PMID: 18035408. 3. Takahashi, ...2007. 318(5858): p. 1917-20. PubMed PMID: 18029452. 5. Stadtfeld, M., et al., Induced pluripotent stem cells... synthetic modified mRNA. Cell Stem Cell, 2010. 7(5): p. 618-30. PubMed PMID: 20888316. PubMed Central...differentiation of pluripotent stem cells. Cell, 2012. 149(5): p. 1174-1174 e1. PubMed PMID: 22632979. 14. Sasaki...

  12. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    ... use Sendai virus to reprogram PBMCs efficiently [5]. However, the Sendai virus method is expensive, especially...establishment of virus-free hiPSCs usually require about 5-10 passages. Furthermore, working with Sendai virus...2003. 302(5644): p. 415-9. PubMed PMID: 14564000. 5. Seki, T., S. Yuasa, and K. Fukuda, Generation of ...pluripotent stem cells. Korean J Intern Med, 2014. 29(5): p. 547-57. PubMed PMID: 25228828. 7. Okita, K., ...p53-p21 pathway. Nature, 2009. 460(7259): p. 1132-5. PubMed  PMID: 19668191. PubMed Central PMCID: PMC2917235...pCXLE-hUL 27080 L-MYC and LIN28 pCXLE-hOCT3/4-shp53-F 27077 OCT3/4 and shRNA against p53 pCXWB-EBNA1... is the expression of the reprogramming factors- OCT3/4, SOX2, KLF4, and C-MYC [1]. For the derivation...

  13. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ... for 5 years and just recently stepped down. I have gained invaluable experience and even after 5 years...their PhD and postdoctoral training. Addgene executive director Joanne Kamens has written a 5-part blog on...in as Chair of the Committee which I then led for 5 years.  Different leadership styles Being an effective...management-for-scientists-managing-vs-leading. The National Postdoctoral Association (NPA) outlines 6 Core Competencies... and Management skills”. The NPA states that Postdoctoral scholars should have the skills and techniques... leadership experience). I was involved with postdoctoral issues first at Brigham and Women’s Hospital...Chair of the Advocacy Committee at the National Postdoctoral Association (NPA). Follow Juliet on Twitter ...

  14. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...modifications, which can all potentially affect function [5]. We and others have reported that FP cysteine residues...properly exiting the ER and fluorescing (see Figure 2) [5].  When using FPs containing two cysteine residues...FP-fusion proteins. Our studies and those of others (5, 6, 7, 10, 11) highlight the very real consequences...published oxidative optimized palette of FPs (moxFPs) [5]. The moxFPs overcome the inherent problems of popular...PMID: 23971632. Pubmed Central PMCID: PMC3806368. 5. Costantini, Lindsey M., et al. "A palette of fluorescent...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz... from Albert Einstein College of Medicine. Her doctoral research was conducted in the laboratory of Erik...

  15. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...this is a great resource to have in your pocket.   5. +AMINO- by iGreenEarth AMINO is a very simple app...Management Software Resources on Addgene.org Find Protoctols for Your Research Catch Up On Your Molecular ...

  16. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...resulting from non-homologous end joining (NHEJ) (2-5), depending on your desired genome edit. Since exploitation...targets. Comparatively, detection limits of about 5% mutant targets have been reported for mismatch nuclease...PMID: 25964300. PubMed Central PMCID: PMC4477672. 5. Sedlak, R. H. et al. Digital detection of endonuclease... triggered by engineered nucleases. G3 (Bethesda) 5, 407–415 (2015). PubMed PMID: 25566793. PubMed Central...This post was contributed by Scott Findlay, a Postdoctoral Fellow at the University of Alberta. If you’... out of frame! Scott Findlay is currently a Postdoctoral Fellow at the University of Alberta. He is interested...

  17. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ..., and a room of 5-30 faculty members. The session lasts up to an hour. You will get 5-10 minutes to present... should be mechanistic, doable by 1-3 people in 1-5 years, and contribute to resolving the overarching...solve the problem. What will you have accomplished in 5-10 years? You need to be able to describe what success...contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia Research Campus of the...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...and now serves as the Director of Student and Postdoctoral Programs at the Janelia Research Campus of the...

  18. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    ...differentiate only into myogenic precursor cells [5]. Classification of stem cells Stem cells are classified... are obtained from the inner cell mass (ICM) of a 5-6 day old human blastocyst [6]. They are isolated ...Gynaecol, 2004. 18(6): p. 827-42. PubMed PMID: 15582541. 5. Seale, P., A. Asakura, and M.A. Rudnicki, The potential...human fibroblasts by defined factors. Cell, 2007. 131(5): p. 861-72. PubMed PMID: 18035408. 10. Yu, J., et... the ectopic expression of four embryonic genes- OCT4, SOX2, KLF4, and C-MYC. iPSCs were first generated...communication and publishing after completing her postdoctoral training from Massachusetts General Hospital...

  19. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...modifications and homologous sequences are encoded at the 5’ end. First design the template binding region, with...insertion site. Encode half the new sequence at the 5’ end of each primer, with an overlap for homologous...primer by adding a short sequence complementary to the 5’ end of the reverse primer. Mutagenesis - Mutagenesis...codon and homologous region can be included at the 5’ end of either primer. Subcloning - To move a gene...recombination events to assemble the final plasmid. At least 5 fragments can be correctly assembled using IVA cloning...our Plasmids 101 blog posts Read our blog post on 5 factors to consider before choosing a cloning method...Laboratory of Molecular Biology. Jake Watson is a postdoctoral researcher in neurophysiology at the MRC Laboratory...

  20. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...postdocs had achieved tenure-track positions within 5-7 years of earning their PhDs (3), showing that the...reflect the number of positions available in academia (5, 6). Academic data and the labor market for PhDs Later...PMID: 25925677. Pubmed Central PMCID: PMC4484856. 5. Bourne, Henry R. "A fair deal for PhD students and...the Future of Research will be held from 22-24 October. This blog has been contributed by guest blogger...strong supporter of increasing the number of postdoctoral scientists at University of Maryland. Paula... can register here or live stream the event on October 22nd and 23rd here. Many thanks to our guest blogger...
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