...self-associating fluorescent domains. ACS Chem Biol. 2013 Oct 18;8(10):2133-9. doi: 10.1021/cb400427b. Epub 2013... neurons and T lymphocytes. Nat Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773. (PubMed) Friedrich et al...

...U, Takahashi M, Gage FH, Verma IM. J Virol. 1998 Oct;72(10):8150-7. PubMed PMID: 9733856. PubMed Central...transactivator for transcriptional activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts... is eliminated through the addition of a chimeric 5’ LTR containing a Tat-independent promoter. Although...PMID: 25075903. PubMed Central PMCID: PMC4486245. 5. Patient monitoring and follow-up in lentiviral clinical...

...Mechanisms, detection, and control. Int J Pharm. 2018 Oct 25;550(1-2):251-268. doi: 10.1016/j.ijpharm.2018.08.043...Edelhoch method. Int J Biol Macromol. 2015;77:260-5. doi: 10.1016/j.ijbiomac.2015.03.027. Epub 2015 Mar... in Colloid & Interface Science, Volume 19, Issue 5, 2014, Pages 438-449, ISSN 1359-0294, https://doi....

...of a plasmid by diagnostic digest . Last Upload: Oct. 11th, 2016 Protocol Video Watch the video below ...Klenow DNA Polymerase I for 3′ overhang removal and 5′ overhang fill-in. If you are using blunt ends or ...

...versus analog real-time PCR. Nat Methods. 2013 Oct;10(10):1003-5. PubMed PMID: 22035192. PubMed Central PMCID...PMID: 20486768. PubMed Central PMCID: PMC2957240. 5.Taylor, Sean C., Genevieve Laperriere, and Hugo Germain...

...Nature Methods. 2013 May 5:10(6):540-7. doi: 10.1038/nmeth.2450. Epub 2013 May 5. PubMed. Zebrabow: multispectral... van Rheenen J, Simons BD, Clevers H. Cell. 2010 Oct 1;143(1):134-44. PubMed. Drosophila Brainbow: a recombinase-based...

... Technologies. Cold Spring Harbor Laboratory, 27 Oct. 2023. Crossref, https://doi.org/10.1016/j.xgen.2024.100519...Coli Synthetic Biology.” ACS Synthetic Biology, vol. 5, no. 10, American Chemical Society (ACS), 2 May 2016...Traditional Biology.” ACS Synthetic Biology, vol. 5, no. 1, American Chemical Society (ACS), 4 Nov. 2015....” PLOS ONE, edited by Alvaro Galli, vol. 13, no. 5, Public Library of Science (PLoS), 30 May 2018, p.... vol. 8, no. 11, American Chemical Society (ACS), 5 Nov. 2019, pp. 2593–606. Crossref, https://doi.org...

...Last updated on Oct 1, 2020 by Aliyah Weinstein. This post was contributed by guest bloggers, Wenning... best case, we saw that 2 out of 3 mice carried a 5 kb insertion in the ROSA locus as assessed by Southern... mouse gene with its human ortholog (greater than 5 kb), it remains to be seen whether CRISPR is as robust...

...ACS Chem Biol 2014 May 16;9(5):1104-12. Heinemann et al., FEBS Lett 2012 Oct 19;586(20):3716-22. Park...transgenesis constructs. Kirchmaier et al., PLOS One 2013 Oct 7;8(10):e76117. Irreversible peptide-peptide ligation...creating a single transcriptional unit (Ex: a promoter, 5’UTR, coding region, and terminator). Next, up to six...e16765. Engler et al., ACS Synth Biol 2014. Feb 5 (Web); DOI: 10.1021/sb4001504. For further reading...Stigleoclonium helveticum (ShChR), Chlamydomonas noctigama (CnChR1), Chloromonas subdivisa (CsChR), Chloromonas...

...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...773–779 . https://doi.org/10.1038/s41477-020-0704-5 Santana I, Wu H, Hu P, Giraldo JP (2020) Targeted ...

... HTR3A 5-hydroxytryptamine (serotonin) receptor 3A 5-HT-3, 5-HT3A, 5-HT3R, 5HT3R, HTR3 HTR3B 5-hydroxytryptamine...heavy diversity 5-24 (non-functional) IGHD524 IGHD5-5 immunoglobulin heavy diversity 5-5 DK4, IGHD55 IGHD6...:14099, K5-5, MGC88293 CCR5 chemokine (C-C motif) receptor 5 CC-CKR-5, CCCKR5, CD195, CKR-5, CKR5, CMKBR5...receptor 3B 5-HT3B HTR3C 5-hydroxytryptamine (serotonin) receptor 3, family member C - HTR3D 5-hydroxytryptamine... interleukin 5 (colony-stimulating factor, eosinophil) EDF, IL-5, TRF IL5RA interleukin 5 receptor, alpha...

...PAmCherry3 570 596 5 6.2 25 min Monomer pPAmCherry3-C1 - Mammalian Expression PAmKate 568 628 5 5.6 19 min Monomer...2014 Vol. 11 No. 5, pp.572-8 Yu et al. : Nature Communications, May 2014 Vol. 15 No. 5, p. 3626 Do you ...pSBFP2-C1 - Mammalian Expression Azurite 383 450 14 5 Prone to dimerization pLV-Azurite - Mammalian Lentiviral...pB18cmBFPAzurite - Bacterial Expression mAzurite 383 450 14 5 Monomer (A206K) mAzurite-N1 - Mammalian Expression...pmTurquoise2-N1 - Mammalian Expression CyPet 435 477 18 5 Monomer pCEP4CyPet-MAMM - Mammalian Expression pCyPet-C1...Structure Plasmids mKusabira-Orange (mKO) 548 559 31 5 4.5 hr Monomer pQC mKorange IX - Mammalian Expression...mRaspberry-N1 - Mammalian Expression TagRFP675 598 675 4 5 Prone to dimerization pTagRFP675-N1 - Mammalian Expression...

...AAV2/5 indicates a virus containing the genome of serotype 2 packaged in the capsid from serotype 5. Use...comparison. Duan D, Yue Y, Engelhardt JF. Mol Ther. 2001 Oct;4(4):383-91. PubMed . Return to Top...efficiency, as well as alter tropism. For example, AAV2/5 targets neurons that are not efficiently transduced...first with a 3’ splice donor and the second with a 5’ splice acceptor. When these viruses co-infect a cell...much less efficient than with a single AAV virus (∼5%). Another technique for increasing packaging capacity...pseudotyped with viral capsids from serotypes 1, 2, and 5 display differential efficiency and cell tropism after...

...Hair Cell-Like Cells. Stem Cells Transl Med, 2016. 5(5): p. 561-71. PubMed PMID: 27013738. PubMed Central...include retrovirus [2], lentivirus [4], adenovirus [5], Sendai virus [6], plasmid electroporation (episomal...in vitro where treatment of mouse fibroblasts with 5-azacytidine led to their conversion into myoblasts...human fibroblasts by defined factors. Cell, 2007. 131(5): p. 861-72. PubMed PMID: 18035408. 3. Takahashi, ...2007. 318(5858): p. 1917-20. PubMed PMID: 18029452. 5. Stadtfeld, M., et al., Induced pluripotent stem cells... synthetic modified mRNA. Cell Stem Cell, 2010. 7(5): p. 618-30. PubMed PMID: 20888316. PubMed Central...differentiation of pluripotent stem cells. Cell, 2012. 149(5): p. 1174-1174 e1. PubMed PMID: 22632979. 14. Sasaki...

... use Sendai virus to reprogram PBMCs efficiently [5]. However, the Sendai virus method is expensive, especially...establishment of virus-free hiPSCs usually require about 5-10 passages. Furthermore, working with Sendai virus...2003. 302(5644): p. 415-9. PubMed PMID: 14564000. 5. Seki, T., S. Yuasa, and K. Fukuda, Generation of ...pluripotent stem cells. Korean J Intern Med, 2014. 29(5): p. 547-57. PubMed PMID: 25228828. 7. Okita, K., ...p53-p21 pathway. Nature, 2009. 460(7259): p. 1132-5. PubMed  PMID: 19668191. PubMed Central PMCID: PMC2917235...pCXLE-hUL 27080 L-MYC and LIN28 pCXLE-hOCT3/4-shp53-F 27077 OCT3/4 and shRNA against p53 pCXWB-EBNA1... is the expression of the reprogramming factors- OCT3/4, SOX2, KLF4, and C-MYC [1]. For the derivation...

... for 5 years and just recently stepped down. I have gained invaluable experience and even after 5 years...their PhD and postdoctoral training. Addgene executive director Joanne Kamens has written a 5-part blog on...in as Chair of the Committee which I then led for 5 years.  Different leadership styles Being an effective...management-for-scientists-managing-vs-leading. The National Postdoctoral Association (NPA) outlines 6 Core Competencies... and Management skills”. The NPA states that Postdoctoral scholars should have the skills and techniques... leadership experience). I was involved with postdoctoral issues first at Brigham and Women’s Hospital...Chair of the Advocacy Committee at the National Postdoctoral Association (NPA). Follow Juliet on Twitter ...

...modifications, which can all potentially affect function [5]. We and others have reported that FP cysteine residues...properly exiting the ER and fluorescing (see Figure 2) [5].  When using FPs containing two cysteine residues...FP-fusion proteins. Our studies and those of others (5, 6, 7, 10, 11) highlight the very real consequences...published oxidative optimized palette of FPs (moxFPs) [5]. The moxFPs overcome the inherent problems of popular...PMID: 23971632. Pubmed Central PMCID: PMC3806368. 5. Costantini, Lindsey M., et al. "A palette of fluorescent...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz... from Albert Einstein College of Medicine. Her doctoral research was conducted in the laboratory of Erik...

...this is a great resource to have in your pocket.   5. +AMINO- by iGreenEarth AMINO is a very simple app...Management Software Resources on Addgene.org Find Protoctols for Your Research Catch Up On Your Molecular ...

...resulting from non-homologous end joining (NHEJ) (2-5), depending on your desired genome edit. Since exploitation...targets. Comparatively, detection limits of about 5% mutant targets have been reported for mismatch nuclease...PMID: 25964300. PubMed Central PMCID: PMC4477672. 5. Sedlak, R. H. et al. Digital detection of endonuclease... triggered by engineered nucleases. G3 (Bethesda) 5, 407–415 (2015). PubMed PMID: 25566793. PubMed Central...This post was contributed by Scott Findlay, a Postdoctoral Fellow at the University of Alberta. If you’... out of frame! Scott Findlay is currently a Postdoctoral Fellow at the University of Alberta. He is interested...

..., and a room of 5-30 faculty members. The session lasts up to an hour. You will get 5-10 minutes to present... should be mechanistic, doable by 1-3 people in 1-5 years, and contribute to resolving the overarching...solve the problem. What will you have accomplished in 5-10 years? You need to be able to describe what success...contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia Research Campus of the...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...and now serves as the Director of Student and Postdoctoral Programs at the Janelia Research Campus of the...