...of a plasmid by diagnostic digest . Last Upload: Oct. 11th, 2016 Protocol Video Watch the video below ...Klenow DNA Polymerase I for 3′ overhang removal and 5′ overhang fill-in. If you are using blunt ends or ...

...resuspended cell pellets and sonicate 5 x 1 sec pulses with at least 5 minutes on ice between each pulse,.... T-175 flask, Corning 430825, 175 cm 2 Cellstack 5, Corning 3319, 3180 cm 2 Cellstack 2, Corning 3269...high glucose, add 55 mL of heat-inactivated FBS and 5 mL of glutaGRO 11 mL of 200 mM L-alanyl-L-glutamine... water + 100 mL of 1 M Tris HCl pH 8.5 + 60 mL of 5 M Sodium Chloride + 4 mL of 1 M Magnesium Chloride...supernatant and resuspend the pellets in a total of 5 mL of cell lysis buffer (recipe above). Pipet back...before these images were taken. Last reviewed on: October 10, 2023...

...the wells. Immediately before use, mix 5 mL TMB Solution and 5 mL Peroxide Solution from the TMB substrate...at room temperature or overnight at 4 °C . Section 5: TMB reaction Carefully remove the plate seal from...micrograms of antigen loaded. Last reviewed on: October 8, 2024...