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We narrowed to 94 results for: Blue fluorescent Protein

Showing: 21 - 40 of 94 results

  1. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...biosensors. NanoLuc®-fluorescent protein fusions for imaging Fluorescent proteins are fantastic imaging... penetrative orange fluorescent protein fusion (OgNLuc). Both fluorescent proteins can be excited by Nluc...of a responsive protein (e.g. calcium binding protein) fused to two fluorescent proteins. When the responsive...transfer of energy from one fluorescent protein to a compatible fluorescent protein. FRET can be measured by...Luciferase Plasmids Browse the Fluorescent Protein Collection Find Fluorescent Protein Biosensors ...NLuc to fluorescent proteins to make better reporters and the second couples NLuc to fluorescent proteins... imaging multiple proteins at the same time in live cells using fluorescent proteins (FPs) can be problematic...

  2. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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    Blog Post
    ...will not produce the fluorescent protein while areas that are exposed will fluoresce. With these images,...BLADE (blue light-inducible AraC dimers in E. coli) that turns expression on and off with blue light. ...domain that dimerizes upon exposure to blue light (Fig. 1). Another blue light-inducible dimerization domain...transcriptional regulator Their system is based on the AraC protein that controls transcription from the PBAD promoter...needed for most applications.   Figure 1: With blue light, the VVD domains of the BLADE dimerize and...media and can’t be contained to a specific area. Blue light, however, is spatially confined and you can... containing the pattern of the image, and shined blue light over the plate. Areas on the plate that are...

  3. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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    Blog Post
    ... awards to early career scientists using fluorescent proteins in their research. These awards help early... in the development and dissemination of fluorescent protein tools. This year, we’ve made some changes...inhibits the neuron (via hM4D). She fused fluorescent protein tags to the receptors to monitor their localization...are co-expressed with a photoconvertible fluorescent protein mEOS4a. Cells containing a sensor that shows...-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3) to study glutamate, a signaling...disease,” says Aggarwal. This biosensor changes fluorescence based on the release of glutamate from neurons...thiol-containing acceptors. These hydropersulfides modulate protein activity and have important roles during oxidative...

  4. Botman-Teusink Yeast FP Collection

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    Collection
    ...overexpress various fluorescent markers and tag your proteins of interest with fluorescent proteins in yeast....create fusions of proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are... S, Lim WA, Thorn KS. Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae...Teusink Lab Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources FP Tagging FP Overexpression...comprehensive collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for...Teusink Lab Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources External Resources Teusink..., Teusink B. In vivo characterisation of fluorescent proteins in budding yeast. Sci Rep. 2019 Feb 19;9...

  5. Download Science-Themed Zoom Backgrounds from Addgene

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    Blog Post
    ...interested in our fluorescent protein background. Download the orange fluorescent protein background.     ...not, you might want to check out Addgene’s Fluorescent Proteins blog topic to learn more about FRET and ...out our plasmid and DNA backgrounds. Download the blue plasmid background.     You can find all of the...with another DNA background option, Download the blue DNA background. Or get the same background as a ...

  6. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...are now available for fluorescent reporting and genetically encoded fluorescent voltage indicators (GEVI...sensor and a mutated version of the pHLuorin fluorescent protein. This GEVI features increased brightness ... Examples of some genes encoding gap junction proteins cx43 GJB1 GJB2 Bioelectric states can alter ... even between many cells (through gap junction proteins which connect the cytoplasm of two cells) can ...controlled by the opening and closing of ion channel proteins, they are not always easy to track at the transcriptional...Arch (green-light activated proton pump) and ChR2 (blue-light activated cation channel) light-activated ...anion-conducting channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization...

  7. Visualizing Genomic Loci with CRISPR-Sirius

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    Blog Post
    ... 2015). To do so, they fused a different fluorescent protein to various Cas9 variants, SpCas9, NmCas9,... or boxB) that each recruits a different fluorescent protein (BFP, GFP, or RFP) to the genomic loci of...MS2-PP7) showing recruitment of different fluorescent proteins. (B) CRISPR-Sirius used to visualize relative...blog Download our CRISPR eBook Learn about fluorescent protein-based tools Read all CRISPR blog posts Resources...The catalytically dead Cas9 protein (dCas9) is well known for its ability to bind DNA targets without...identical hairpins within one gRNA will result in a blue, green, or red readout. But gRNAs containing two...boxB sequences inserted into the sgRNA generates blue, green, or red colors for labeling. A combination...

  8. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...when paired with appropriate fluorescent optics. Existing red fluorescent voltage indicators, however,...inhibitory rhodopsin (Ace2N) with the red fluorescent protein (mScarlet) with either a seven or eight amino...manipulations when combined with existing green-fluorescent GEVIs and blue-light-sensitive channelrhodopsins. Find...zebrabow. In this system, cells express various fluorescent proteins controlled by Cre recombination. Each cell...of colors depending on the combination of fluorescent proteins expressed. CreLite-mediated recombination...editors that can be reconstituted in vivo to form a protein identical in sequence to the unmodified enzyme....31937940. Detection of neural activity using a red fluorescent genetically encoded voltage indicator Article...

  9. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post
    ...Photoactivatable Fluorescent Proteins Control Protein Expression with Cre-lox Control Protein Activity with... other kinases and proteins. Zhou et al suggest that more photoswitchable proteins could potentially be... reporter plasmids Find photoactivatable fluorescent proteins in empty backbones in the Michael Davidson...Photoswitchable Fluorescent Proteins ... fluorescent proteins...transduction pathways are a lot like cell phone networks. Protein kinases deliver messages to the next members of... the active site. Dronpa also produces green fluorescence when exposed to 500 nm light.  Under 400 nm ...

  10. Viral Vectors 101: Calcium Sensors

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    Blog Post
    ...indicators (GECIs) are fluorescent proteins linked to calcium binding proteins (e.g., calmodulin or troponin...methods Many calcium reporters use a single fluorescent protein (FP), requiring only a simple measurement.... This sensor switches from green fluorescence to a red fluorescent state only by simultaneous (1) user-controlled...Structure-and mechanism-guided design of single fluorescent protein-based biosensors. Nature chemical biology...Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral Services  ...troponin C). Their fluorescent readouts are used to monitor calcium concentrations and transients in living... of the sensor, and (3) subsequent change in fluorescence only occurs at high calcium concentrations. ...

  11. Optogenetics Plasmid Collection

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    Collection
    ...Chemogenetics Plasmids Chemogenetics Guide Fluorescent Protein Guide Microbial Opsin Plasmids Optical Switch... activity, scientists generally attach a fluorescent protein to the opsin in order to track its expression...types of proteins can also be controlled by light, generally by utilizing a light sensitive protein from ...light-oxygen-voltage-sensing (LOV) proteins. These fusions can then be used as an optical switch to control protein interactions...Plasmids Background Optogenetics plasmids encode proteins that are controlled by light. Many of these plasmids... opened and closed in response to light. These proteins can be expressed in specific neurons, often utilizing... is a membrane channel that opens in response to blue light and allows cations to flow into the cell. ...

  12. SunTag and Fluorescent Imaging

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    Blog Post
    ... a given protein is to fuse it to a fluorescent protein. You can then study where the protein is localized... Sign Up to Receive the Fluorescent Protein eBook Avoid Fluorescent Protein Oligomerization Learn about... Addgene.org Find Fluorescent Protein Empy Backbones Visit Our Fluorescent Protein Guide Page Find Luciferase... the brightness in your fluorescent imaging experiments. Fluorescent protein fusions One of the easiest...problems? Instead of directly fusing a fluorescent protein to your protein of interest, you instead fuse it ...on a protein-by-protein basis. However, since many of Addgene’s SunTag plasmids have coveted “blue flames...overexpress your protein at higher levels, you risk observing artifacts only present when the protein is at a ...

  13. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post
    ...many fluorescent proteins. Additional fluorescent protein tools Photoactivable fluorescent proteins (proteins...Laser include: Blue-green fluorescent proteins like EGFP Yellow-orange fluorescent proteins like TagRFP,...Choosing Your Fluorescent Protein Learn Some of the Pitfalls of Working With Fluorescent Proteins Use FRET ...Monitor Protein-Protein Interactions Resources on Addgene.org Check out Our Fluorescent Protein Guide ...the visualization of fluorescent proteins expressed in the specimen.  2. Fluorescence Activated Cell Sorter...using cells labelled by fluorescent chemicals or by other fluorescent proteins, labs were also able to...that fluoresce after a light-induced chemical reaction) or photoconvertible Keade proteins (protein that...

  14. Quick Guide to Near-Infrared Fluorescent Proteins

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    Blog Post
    ...al. A naturally monomeric infrared fluorescent protein for protein labeling in vivo. Nat Methods 12, 763... Medicine. Several sets of near-infrared fluorescent proteins (NIR FPs) and biosensors have been created...Addgene. Biliverdin chromophore Near-infrared fluorescent proteins (NIR FPs) were derived from natural photoreceptors... about cellular events via  fluorescence changes occurring due to protein synthesis, degradation, or reconstitution...Shcherbakova et al., 2018). For detection of protein-protein interactions, we recommend using NIR split ...expertise is development and applications of fluorescent proteins, genetically encoded biosensors, and optogenetic... to Increase Brightness of Near-Infrared Fluorescent Proteins in Mammalian Cells. Cell Chem Biol 24, 758...

  15. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...Choosing Your Fluorescent Proteins for Multi-Color Imaging Choose the Brightest Fluorescent Protein for Your...Addgene.org Browse Our Fluorescent Protein Resources Find Empty Fluorescent Protein Backbones Find Plasmids...super-resolution (3). Fluorescence microscopy techniques   Fluorescent Widefield Microscopy Fluorescent Widefield...block out of focus fluorescence from reaching a detector or restricting fluorescence to specific excitation... of blue to excite a molecule of GFP. Because red light has approximately half the energy of blue light... fluorescent proteins... into noise. Due to its unrivaled contrast, fluorescence imaging has emerged as the dominant light microscopy...

  16. Bacterial Expression Systems

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    Collection
    ...Photoactivatable Fluorescent Proteins Fluorescent Protein Timers Return to top Protein Interactions Förster...Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to top Protein Visualization... Split Fluorescent Proteins for Studying Protein–Protein Interactions Return to top Controlled Expression...that can be used for protein purification, protein visualization, study of protein interactions, controlled...Bacterial Expression Systems Protein Purification Protein Visualization Protein Interactions Controlled Expression...plasmids that contain fluorescent protein (FP) tags so you can express your protein of interest fused to... Additional Addgene Protein Visualization Resources Visit our Fluorescent Protein Guide to find an extensive...

  17. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...cleaves the supplied fluorescently labelled ssDNA substrates. Upon cleavage, the fluorescence is no longer quenched...resulting fluorescence can be observed under either no excitation light, UV light or LED blue light.  Highlights... cleavage of fluorescently labelled RNA, the authors employ a P51 cardboard fluorescence visualizer that... 9V battery which uses a Blue LED and an orange filter to detect fluorescence upon cleavage. Highlights...streptavidin portion of the probe via biotin. The fluorescent portion of the probe that is cut will continue...and released when cleaved. Cleavage results in fluorescence and this approach can be incorporated into a...90 minutes Equipment needed: 37°C heat block, LED blue light illuminator or UV light illuminator. Approach...

  18. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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    Blog Post
    ...post from Joachim Goedhart: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection...Updated May 10, 2021. In the world of fluorescent proteins and their use for imaging cell biology, Michael...searchable format on Addgene’s Michael Davidson Fluorescent Protein Collection webpage. These plasmids are divided... tagged with mEmerald. Plasmids to monitor protein-protein interactions through multicolor labeling and... plasmid backbones spanning a rainbow of fluorescent proteins “It’s a great collection with over 300 backbones... impressively big deposit. “He has every fluorescent protein under the sun, from the standard oldies but...interest isn’t in the ORF collection, the fluorescent proteins are, which makes mixing and matching a relatively...

  19. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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    Blog Post
    ...b(right)est fluorescent proteinFluorescent Proteins 101: A History of Fluorescent Proteins  ...stability of fluorescence over time is crucial. For instance, the fluorescence of fluorescein isothiocyanate...principles of fluorescent dyes/ligands Figure 1: Example structures of fluorophores, fluorescent dyes,...dyes, and fluorescent ligands. Figure created with biorender.com. Fluorescence refers to the emission...attached to self-labeling proteins. Some common examples of these self-labeling proteins would be SNAP-tag® ...through fluorescent labeling. There are several advantages to using these self-labeling proteins over traditional... traditional fluorescent protein tags, such as higher specificity, increased versatility in probe attachment...

  20. Antibodies 101: Designing Your First Flow Panel

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    Blog Post
    ...inside of your cells. While proteins themselves can emit intrinsic fluorescence when excited with ultraviolet...amino acids found in all proteins, so you can't distinguish the different proteins from each other. To distinguish... A common method of labeling proteins is to fuse them with a fluorescent reporter, such as GFP or mCherry...through detecting the reporter's fluorescence. Another way to label proteins is through treating cells with...bright" for the blue laser. To understand why that is, let's look at Figure 2. The blue laser excites at...distinguish different target proteins in your flow analysis, you'll need to use fluorophores that attach...mCherry, through genetic modification. As your protein of interest will only occur in combination with that...
Showing: 21 - 40 of 94 results