We narrowed to 30 results for: CRE-ER

...References 1. Snapp, E.L., et al., Formation of stacked ER cisternae by low affinity protein interactions. J... second FP, often decreasing the fluorescent signal from the first FP and increasing the signal from the...an FP to a transmembrane protein significantly increases the effective concentration of the FP. The FP ...to a plane and generally only rotates in 360°, increasing the probability that two FPs will collide with...polymer formation as fusion protein concentration increases. The polymerized fusion proteins that form as ... His interests include the quality control of secretory proteins in the Endoplasmic Reticulum and live...

...But CRISPRing typical mouse models, including creating Cre-dependent conditional alleles, has remained ...fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and tetracycline drug induction systems...how you can make CRISPR mouse model generation easi-er! Generating mice before CRISPR The process of generating...plasmid pmScarlet_C1! After creating the T7-DNA template, you'd use ivTRT to create the ssDNA construct as ...CRISPR genome editing has made it easier to create knockout alleles in a variety of species, including...repair template is used. The original design for creating a conditional allele, or floxed mouse, from Yang... and inject the ES cells into a mouse embryo to create chimeric mice. This laborious process takes ~8-...

...R., & Okiyoneda, T. (2023). UBE3C Facilitates the ER-Associated and Peripheral Degradation of Misfolded...of protein activity. Together, these limitations create challenges for measurements needed to move discoveries...means to quickly answer research questions before creating knock-ins.  Figure 1: Schematic of HiBiT...Functional assays further showed that imported ADAR1 increased oncogenic editing of its most common target, AZIN1...UBE3C. Degradation of CFTR was measured through decreases in HiBiT signal, which was slowed by knockdown...factors present unique challenges for study. These secreted signaling proteins regulate vital cell and tissue...physiologically and endogenously relevant models, tools that increase confidence become more important. For many difficult-to-study...

...Blue-wavelength fluorescent protein Activity Cre dependent Flp dependent Cre and Flp dependent Constitutive Serotype...Arch GFP Cre dependent 1, 9 Edward Boyden 28305 pAAV-FLEX-ArchT-tdTomato CAG ArchT tdTomato Cre dependent... EYFP Cre dependent 9 Karl Deisseroth 26966 AAV-Ef1a-DIO eNpHR 3.0-EYFP EF1a eNpHR 3.0 EYFP Cre dependent...soma-targeted) mRuby3 Cre dependent 9 Hillel Adesnik 137155 pAAV-nEF-Con/Fon-iC++-EYFP nEF iC++ EYFP Cre and Flp dependent...eOPN3 mScarlet Cre dependent 1, 5 Ofer Yizhar 139504 pAAV-CAG-DIO-PPO-Venus CAG PPO Venus Cre dependent 5...198516 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE EF1a PdCO mScarlet Cre dependent 1, 5 Ofer Yizhar 202199...137142 pAAV-nEF-Con/Fon-ChR2-mCherry nEF ChR2 mCherry Cre and Flp dependent 8 Karl Deisseroth 137143 pAAV-nEF-Con...

...results, so you only need to pick your favorite (...er, whichever one uses assumptions that match your data...Welcome to our deep dive series, which aims to increase your understanding and technical proficiency with...temperature drops, single intensity should smoothly increase as the DNA amplicon transitions from single-stranded...finally, drop once more during a final temperature increase. If there is a single amplicon, there should only...c) A double peak indicating 2 amplicons. Image credits (respectively): Zuzanna K. Filutowska; Selma, 2019...can fit in a quick game of Connect Four! Image created in BioRender.   Passive Reference Dyes Whatever...

...combined with site specific recombinase technology like Cre-lox, which allows you to express an opsin ONLY in...using a viral vector with a floxed opsin in a VGAT-cre animal will result in expression of the opsin only...Alternatively, when one half of a given protein, such as the Cre recombinase, is fused to Cry2 and the other half ...with addition of trafficking signal from Kir2.1 and ER export signal provide improved membrane targeting... include: Increased photocurrent amplitude Examples: ChR2(H134R), C1V1(t/t), ChIEF Increased channel (... following enhancements: Increased light sensitivity Example: ArchT Increased photocurrent amplitude Examples...Scherffelia dubia ). Synthetic variants have been created via genetic point mutations, codon optimization...

...expression, and an ER signal/leader sequence (L) for translocation of the heavy chain across the ER membrane. ...expression, and an ER signal/leader sequence (L) for translocation of the light chain across the ER membrane. ..., HEK293, COS, CHO), from which the R-mAbs are secreted into the culture media. For high level expression... Gavira-O'Neill CE, et al. 2020. Development, Screening, and Validation of Camelid-Derived Nanobodies ...

...this screen the authors were able to identify 7 previously unsuspected genes with known roles in ER-associated...loxP-stop-loxP cassette into the Rosa26 locus, allowing for Cre-dependent expression of a GMAP inserted gene. The...of a good screening experiment can be found on our blog post entitled “Genome-wide Screening Using CRISPR...Receptor-ome Expression and Screening via Transcriptional Output-TANGO (PRESTO-TANGO) to screen the NCC-1 library...and later create stable cell lines expressing it. The Church lab fixed this issue by creating a new dCas9...  CRISPR pooled libraries enable researchers to screen the entire genome for genes that regulate a wide...CRISPR knockout library and used this library to screen for genes involved in West Nile Virus (WNV) induced...

...Lenti-sgAtm#1/Cre ATM CMV Ataxia telangiectasia Charles Swanton 173580 Lenti-sgAtm#2/Cre ATM CMV Ataxia... Dong-Er Zhang 12421 pMCSF-R(mA)-luc CSF1R CSF1R Hereditary diffuse leukoencephalopathy Dong-Er Zhang ... Dong-Er Zhang 12424 pMCSF-R(DD)-luc CSF1R CSF1R Hereditary diffuse leukoencephalopathy Dong-Er Zhang ...Lewy body dementia Tiago Outeiro 89643 Lenti-sgAtm/Cre ATM U6 Ataxia telangiectasia Monte Winslow 89755 ...T5 Parkinson's Suzanne Pfeffer 208392 Lenti-sgRNA-Cre-GpNLuc-sgmATM ATM Ataxia telangiectasia Gaorav Gupta...CSF1R Hereditary diffuse leukoencephalopathy Dong-Er Zhang 13313 pCMVTNT PINK1 N-myc PINK1 Myc CMV Parkinson's...Spinocerebellar ataxia 19 James Trimmer 129409 Slc1a3-CreERT2 Targeting Vector SLC1A3 PGK Episodic ataxia Walker...

... 1 DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1 ESR2 estrogen receptor 2 (ER beta) ER-BETA, ESR-BETA, ESRB...erythropoietin receptor MGC138358 EREG epiregulin ER ESM1 endothelial cell-specific molecule 1 endocan...Upon activation, NK cells secrete perforin and granzyme B, two enzymes that create pores within an infected...LSIGN, MGC129964, MGC47866 CREB1 cAMP responsive element binding protein 1 CREB, MGC9284 CTSB cathepsin ...chromogranin A (parathyroid secretory protein 1) CGA CHGB chromogranin B (secretogranin 1) SCG1 CKLF chemokine-like... SDS, SWDS SCG2 secretogranin II (chromogranin C) CHGC, SN, SgII SCGB3A1 secretoglobin, family 3A, member...HIN1, LU105, MGC87867, PnSP-2, UGRP2 SCT secretin - SCTR secretin receptor SR SCYE1 small inducible cytokine...