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We narrowed to 78 results for: Drosophila

Showing: 21 - 40 of 78 results
  1. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    .... They can also be introduced directly into a Drosophila S2 cell line stably expressing Cas9. This PCR...Comprehensive Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells.” G3 (Bethesda) 6(6) (2016...
  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...the Drosophila community as well. The pooled library, designed to be used with cultured Drosophila cells...Mol Biol. 2014. PubMed PMID: 24395366 Oxford Drosophila CRISPR library Addgene has had the privilege ...expression backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter...rather than a mixed sample. TRIBE was developed in Drosophila, and further work is needed to assess its function...plasmids optimized for dCas9-VPR expression in drosophila and yeast. Chavez A, et al. Nat. Methods 2015...
  3. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...Fragmid was developed for mammalian systems and Drosophila (McGee et al., 2023). Fragmid developers noticed...described above works in both mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate...
  4. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...Is there any AAV serotype that can be used in Drosophila? A: Not to our knowledge. Disease Applications...
  5. Plasmids 101: Inducible Promoters

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    Blog Post
    ...genome engineering in species like C. elegans and Drosophila. Light inducible promoters Light is another way...
  6. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...OsU3 pegRNA No Yiping Qi 149545 pCFD3-NS Drosophila Drosophila U6:3 pegRNA BbsI No Vermilion Norbert Perrimon...Perrimon 149546 pCFD5-NS Drosophila Drosophila U6:3 pegRNA + nicking sgRNA BbsI No Vermilion Norbert Perrimon...CRISPR Plasmids: Prime Edit Mammalian Bacteria Drosophila Plant Empty pegRNA Resources Prime editing is...Selectable Marker PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker...
  7. CRISPR Plasmids - Drosophila

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    Collection
    ... CRISPR Drosophila CRISPR Plasmids: Drosophila Browse CRISPR...designed for use in Drosophila and other insects. CRISPR...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...CRISPR plasmids have been designed for use in Drosophila and other insects. Cut Fully functional CRISPR...
  8. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...systems and Drosophila. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid ...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...
  9. CRISPR Plasmids - Tagging

    Type
    Collection
    ... or C-terminal tagging in Drosophila cells. N terminal tagging in Drosophila cells 3.2 MB C terminal tagging...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR... Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System The Förstemann lab has developed...developed a CRISPR tagging technique for use in Drosophila cells that uses PCR to generate both an expression... tagging in Drosophila cells 3.3 MB Seydoux C. elegans Tagging System Geraldine Seydoux's lab has developed...
  10. Hot Plasmids - October 2020

    Type
    Blog Post
    ... RNA targeting Cas system that can be used in Drosophila melanogaster. Get the plasmids at Addgene The...
Showing: 21 - 40 of 78 results