We narrowed to 78 results for: Drosophila
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TypeBlog Post.... They can also be introduced directly into a Drosophila S2 cell line stably expressing Cas9. This PCR...Comprehensive Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells.” G3 (Bethesda) 6(6) (2016...
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27 Hot Plasmids from 2016
TypeBlog Post...the Drosophila community as well. The pooled library, designed to be used with cultured Drosophila cells...Mol Biol. 2014. PubMed PMID: 24395366 Oxford Drosophila CRISPR library Addgene has had the privilege ...expression backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter...rather than a mixed sample. TRIBE was developed in Drosophila, and further work is needed to assess its function...plasmids optimized for dCas9-VPR expression in drosophila and yeast. Chavez A, et al. Nat. Methods 2015... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog Post...number of model organisms: mammalian cells, yeast, drosophila, plants, worms, zebrafish, frogs, some bacteria... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...increases knock-in efficiency. Finally, as shown in Drosophila S2 cells (Böttcher et al., 2014), inactivation...PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post...Halstead et al. used TRICK to examine translation of Drosophila patterning gene oskar. Early in development, ... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog Post... his calcium indicators can be used in mouse, Drosophila, C. elegans, and zebrafish – essentially all ... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post...Fragmid was developed for mammalian systems and Drosophila (McGee et al., 2023). Fragmid developers noticed...described above works in both mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate... -
AAV Q&A with Tim Miles
TypeBlog Post...Is there any AAV serotype that can be used in Drosophila? A: Not to our knowledge. Disease Applications... -
Plasmids 101: Inducible Promoters
TypeBlog Post...genome engineering in species like C. elegans and Drosophila. Light inducible promoters Light is another way... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...which it was previously not an option, including Drosophila and C. elegans. Find the TurboID plasmids here... -
CRISPR Plasmids - Prime Edit
TypeCollection...OsU3 pegRNA No Yiping Qi 149545 pCFD3-NS Drosophila Drosophila U6:3 pegRNA BbsI No Vermilion Norbert Perrimon...Perrimon 149546 pCFD5-NS Drosophila Drosophila U6:3 pegRNA + nicking sgRNA BbsI No Vermilion Norbert Perrimon...CRISPR Plasmids: Prime Edit Mammalian Bacteria Drosophila Plant Empty pegRNA Resources Prime editing is...Selectable Marker PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker... -
CRISPR Plasmids - Drosophila
TypeCollection... CRISPR Drosophila CRISPR Plasmids: Drosophila Browse CRISPR...designed for use in Drosophila and other insects. CRISPR...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...CRISPR plasmids have been designed for use in Drosophila and other insects. Cut Fully functional CRISPR... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...GCaMP6 only). The genetic elements required for Drosophila studies have been added for jGCaMP7 and jGCaMP8... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...includes systems for tagging mammalian cell lines, Drosophila cells, and C. elegans. In addition to generating... -
CRISPR Plasmids - dCas9-FokI
TypeCollection...systems and Drosophila. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid ...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR... -
CRISPR Plasmids - Tagging
TypeCollection... or C-terminal tagging in Drosophila cells. N terminal tagging in Drosophila cells 3.2 MB C terminal tagging...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR... Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System The Förstemann lab has developed...developed a CRISPR tagging technique for use in Drosophila cells that uses PCR to generate both an expression... tagging in Drosophila cells 3.3 MB Seydoux C. elegans Tagging System Geraldine Seydoux's lab has developed... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post...by members of the worm community but there are Drosophila and zebrafish articles published there as well... -
Hot Plasmids - October 2020
TypeBlog Post... RNA targeting Cas system that can be used in Drosophila melanogaster. Get the plasmids at Addgene The... -
Making CRISPR Plasmids Using Fragmid
TypeBlog Post...your vector, which are: Organism (Mammalian or drosophila) Delivery type (lentivirus, AAV, transposon,... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...can be used to replace genes in yeast, mice, or Drosophila through homologous recombination. The concept...