We narrowed to 87 results for: Drosophila

1. Fluorescent Tagging of Endogenous Genes with SapTrap

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   .... They can also be introduced directly into a Drosophila S2 cell line stably expressing Cas9. This PCR...Comprehensive Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells.” G3 (Bethesda) 6(6) (2016...

2. 27 Hot Plasmids from 2016

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   ...the Drosophila community as well. The pooled library, designed to be used with cultured Drosophila cells...Mol Biol. 2014. PubMed PMID: 24395366 Oxford Drosophila CRISPR library Addgene has had the privilege ...expression backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter...rather than a mixed sample. TRIBE was developed in Drosophila, and further work is needed to assess its function...plasmids optimized for dCas9-VPR expression in drosophila and yeast. Chavez A, et al. Nat. Methods 2015...

3. Some Like it Hot: Thermostable GeoCas9

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   ...number of model organisms: mammalian cells, yeast, drosophila, plants, worms, zebrafish, frogs, some bacteria...

4. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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   ...increases knock-in efficiency. Finally, as shown in Drosophila S2 cells (Böttcher et al., 2014), inactivation...PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https...

5. Visualizing Translation at the Single Molecule Level

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   ...Halstead et al. used TRICK to examine translation of Drosophila patterning gene oskar. Early in development, ...

6. Hot Plasmids: FRET-Based Biosensors

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   ... his calcium indicators can be used in mouse, Drosophila, C. elegans, and zebrafish – essentially all ...

7. Plasmids 101: Modular Cloning Applications and Kits

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   ...Fragmid was developed for mammalian systems and Drosophila (McGee et al., 2023). Fragmid developers noticed...described above works in both mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate...

8. Plasmids 101: Inducible Promoters

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   ...genome engineering in species like C. elegans and Drosophila. Light inducible promoters Light is another way...

9. AAV Q&A with Tim Miles

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   ...Is there any AAV serotype that can be used in Drosophila? A: Not to our knowledge. Disease Applications...

10. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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    ...which it was previously not an option, including Drosophila and C. elegans.   Find the TurboID plasmids here...

11. Plasmids for Endogenous Gene Tagging in Human Cells

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    ...includes systems for tagging mammalian cell lines, Drosophila cells, and C. elegans. In addition to generating...

12. Viral Vectors 101: Calcium Sensors

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    ...GCaMP6 only). The genetic elements required for Drosophila studies have been added for jGCaMP7 and jGCaMP8...

13. Plasmids 101: Knockout/Knock-In Plasmids

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    ...can be used to replace genes in yeast, mice, or Drosophila through homologous recombination. The concept...

14. Open Resources and Plasmid Tools For Studying C. elegans

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    ...by members of the worm community but there are Drosophila and zebrafish articles published there as well...

15. Hot Plasmids - October 2020

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    ... RNA targeting Cas system that can be used in Drosophila melanogaster. Get the plasmids at Addgene The...

16. Making CRISPR Plasmids Using Fragmid

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    ...your vector, which are: Organism (Mammalian or drosophila) Delivery type (lentivirus, AAV, transposon,...

17. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    ...activation levels than SAM in some loci across Drosophila cell lines (Sajwan et al., 2019). SPH SPH combines...

18. Viral Vectors 101: Voltage Indicators

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    ...sources – voltage-gated potassium channels found in Drosophila to phosphatases from sea squirts. This design...

19. Of Myc and Men

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    ...genes: deletion mapping functional domains of Drosophila hsp 70. Embo J 3:3087–3093. https://doi.org/10.1002...

20. PhD Applications After COVID

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    ...Washington University whose research focuses on Drosophila-parasite pathogen interactions.  ...