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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 447 results for: ENA;

Showing: 21 - 40 of 447 results

  1. Promega Plasmid Collection

    Type
    Collection
    ...exceptionally bright and stable luciferase that enables highly sensitive detection at endogenous expression... HiBiT is a small, multifunctional epitope tag enabling precise protein detection. It can be paired with...the presence of a substrate. NanoBiT PPI assays enable kinetic, real-time analysis of protein interaction...

  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...With proper experimental design, biosensors can enable investigation of a signaling pathway or measurement...ligands A modular chemigenetic calcium indicator enables in vivo functional imaging with near-infrared light...genetically encoded ratiometric calcium sensor enables quantitative measurement of the local calcium microdomain...NADPH concentration Semisynthetic sensor proteins enable metabolic assays at the point of care. Science....measurements of membrane charges A FRET sensor enables quantitative measurements of membrane charges in...

  3. Zebrafish Plasmid Collection

    Type
    Collection
    ... is easy to culture large populations that are amenable to genetics, high-throughput screening and non-invasive...Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference (CRISPRi) modulation...optogenetically controlled Cre/loxP system that enables precise temporal and spatial control of gene expression...

  4. Lentivirus Plasmids

    Type
    Collection
    ...viruses also integrate stably into the host genome, enabling long term transgene expression. There are some...lentivirus for ORF expression, multicistronic design enables tracking of viral transduction. Also see plasmid...for inducible knockdown, multicistronic design enables tracking of viral transduction. See article for...

  5. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TTGCTCCTTGGTTGCATCtctgtcttcattatcaCAAACATTTTCTTCATTA tetmethylcytosinedioxygenase 1 TAL3572 & TAL3573 TCAACTCGCGCATCCACAaagcgaaatgttaagaGGGTGAAGGCTTCCATGA...TCAACTCGCGCATCCACAaagcgaaatgttaagaGGGTGAAGGCTTCCATGA tetmethylcytosinedioxygenase 3 TAL3574 & TAL3575 TTATTAGGCACTTTTTTGtaaacgctgtattccaTGCACTTTCCTGTCCACA...TTATTAGGCACTTTTTTGtaaacgctgtattccaTGCACTTTCCTGTCCACA tetmethylcytosinedioxygenase 3 (previous si:ch211-220a11.1) TAL3576...

  6. CRISPR History and Development for Genome Engineering

    Type
    Collection
    .... This prokaryotic immune system is especially amenable to genome engineering, offering flexibility and...target CRISPR to a given locus makes it especially amenable to genetic screens. Pooled gRNA libraries can ... providing tips from experts in the field, and enabling access to multiple plasmids used for various platform...

  8. Gibson Assembly Protocol

    Type
    Protocol
    ... assembled antibiotic gene. This trick can also enable replacement of "inverse PCR" reactions with a two-part...

  10. Plan Your Experiment

    Type
    Collection
    ...alindromic R epeats) is a powerful system that enables researchers to manipulate the genome with relative...Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the efficient mRNA delivery into the mouse zygotes.... Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro...

  11. Pipetting Protocol

    Type
    Protocol
    ...molecular biology. You’ll use a pipette in many scenarios to accurately dispense small amounts of liquid...

  12. Brain Initiative Collection

    Type
    Collection
    ...development and application of innovative tools enabling large-scale real-time interrogation and monitoring... soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics...-soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics...

  13. TALEN Engineering

    Type
    Collection
    ...Targeter (Link opens in a new window) is available to enable users to identify both dimeric TALEN and monomeric...FLASH assembly. This new version of ZiFiT Targeter enables users to search their sequences of interest for...

  15. Viral Vector Guides and Plasmids

    Type
    Collection
    ...dividing and non-dividing cells. These virusal vectors enable long-term stable gene expression and are permanently...They integrate permanently into the host genome, enabling long-term transgene expression. Retroviral vectors...

  16. Plant Plasmids and Resources

    Type
    Collection
    ...CRISPR resources include: A Multipurpose Toolkit enables targeted, specific modification of monocot and ... plants (including tobacco and potato) - Scott Lenaghan. Joint Modular Cloning (JMC) Toolkit includes ...

  17. TALEN Plasmids and Kits

    Type
    Collection
    ... used for single-strand annealing assay, which enables the evaluation of TALEN activities in cultured ...-VP64 Golden Gate TALEN 2.0 47874 pTALYM3 Maria-Elena Torres-Padilla These plasmids were designed for ...

  18. AAV Production in HEK293 Cells

    Type
    Protocol
    ...cells. Pass the cells twice a week during the maintenance phase and do not allow cells to reach 100% confluence...

  19. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...sites, Flp with FRT sites, Dre with rox sites), enabling precise genetic manipulation without cross-reactivity...recombination events in a single experimental system, enabling multi-layered genetic control. Recombinase-mediated...

  20. CRISPR References and Information

    Type
    Collection
    ...the analysis of genome editing experiments. It enables rapid and intuitive interpretation of results produced...current version supports SpCas9, SpCas9-VQR, and enAsCas12a Cas enzymes. Developed by the Manuel Leonetti...
Showing: 21 - 40 of 447 results