We narrowed to 146 results for: Endog

... possible to make the protein specific to any endogenous RNA without tagging,” she said. “It opens a new... new avenue of modifying and manipulating endogenous eukaryotic RNA.” Endless possibilities with PUF proteins...for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. doi: 10.1186...

...-plant cells, which interacts with the cells’ endogenous ubiquitin ligase, the Kanemaki lab was able to...previously developed a CRISPR/Cas9 based method to tag endogenous proteins in HCT116 cells with mAID. Using this...have designed means of studying the effects of endogenous looping on distal promoters and enhancers, or...

... is to tag an endogenous gene. By imaging cells (or tissues) that produce an endogenous protein tagged...employing co-expressing fluorescent proteins or using endogenous tagged genes) can be used to verify performance...

... Raf1, and CDK5 all behave similarly to their endogenous counterparts. Additionally, dimeric Dronpa is... a target for MEK1 phosphorylation, much like endogenous ERK, and its localization serves as an indicator.... Zhou et al studied the negative feedback on endogenous MEK1/2 phosphorylation by creating a short pulse...

...Biology Find the AsCas12f1 plasmids   Selective endogenous encapsidation for cellular delivery of RNAs By...in the capsid, in an approach called Selective Endogenous eNcapsidation for cellular Delivery (SEND). They...plasmids    Figure 1: SEND combines an endogenous Gag homolog, cargo RNA, and fusogen in a modular...

...conjugation. 1) Endogenous lysine residues, which can be found throughout the antibody. 2) Endogenous cysteines...the disulfide bonds between antibody chains. 3) Endogenous carbohydrates added to antibodies post-translationally...

...cell which will recombine the template with the endogenous gene. Here, we'll describe the techniques and...Cre recombinase. If GFP is under control of an endogenous promoter, you can use expression GFP to track...responds. You can also use this method to tag an endogenous protein with GFP, as seen in blue flame plasmid...

...Plasmids for Endogenous Gene Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging...plasmids can be used to create cell lines with endogenously-tagged cellular structures. Addgene ID Plasmid...

... rely on exogenous enzymes and those that use endogenous homologous recombination. Modifying bacterial...and homologous recombination As an alternative, endogenous homologous recombination enzymes can also accomplish... To allow manipulation of bacteria without an endogenous rhamnose regulon, we inserted a copy of rhaS ...

...Piwi-interacting RNA (piRNA) miRNA and piRNA can be found endogenously in many cell types, while shRNA and siRNA are... viral vectors or plasmids, shRNAs hijack the endogenous miRNA machinery for processing (Figure 1). The...structures to create a mature siRNA duplex. Fun fact! Endogenous miRNA production starts with transcription of...

...resulting in processing by the endogenous RNAi machinery. Alternatively, endogenous miRNA precursors can be ...Although similar, amiRNAs are engineered from endogenous precursors of microRNAs found in the genome, ...

...Several labs have also controlled Acr activity with endogenous cell-type specific miRNAs to restrict CRISPR ... Browse Plasmids: Double-Strand Break (Cut) , Endogenous Tagging CRISPR Base Editing To overcome low HDR..., and the break can be repaired by the cell’s endogenous repair mechanisms. Figure 14: Overview of editing...repression gRNA G uide RNA , a synthetic fusion of the endogenous bacterial crRNA and tracrRNA that provides both...Jaenisch, R. (2013). Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional...Adamson, B. (2024). Improving prime editing with an endogenous small RNA-binding protein. Nature . 628 (8008...

...encoded, photocaged Cas9 system could silence endogenous gene expression - demonstrating light-induced...2019) LADL: light-activated dynamic looping for endogenous gene expression control. Nat Methods 16:633–639...light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol 11:198–200 . https...

...transgenes and the up-regulation of corresponding endogenous pluripotency genes. Retroviral vectors are prone... and promoter demethylation and expression of endogenous pluripotency genes (i.e. Oct4) are other measures...Virus-Based Vector Using a Nonstructural C Protein and Endogenous MicroRNAs." Plos One 11, no. 10 (2016). PubMed...

...invader ( Figure 1 ). Figure 1: An overview of the endogenous Type II bacterial CRISPR system. Within the bacterial...allows us to create a variety of modifications in endogenous loci with unprecedented speed and specificity... Tag : Multiple methods make it easier to tag endogenous loci with epitope tags or fluorescent markers...

.... To avoid secondary effects from repurposing endogenous systems that respond to natural stimuli like ...al., 2011), engineered to eliminate binding of endogenous mammalian transcription factors to further minimize...their sequence and eliminating interactions with endogenous factors. Experimental Considerations Expression...

...it as “a novel mode of optogenetic control of endogenous cellular processes." The LITE system can now ...Konermann et al. "Optical control of mammalian endogenous transcription and epigenetic states." Nature....

...coelenterazine, followed by the addition of the endogenous ligand or drug. In the inactive state, the luminescent...different GPCRs. This collection can be used to study endogenous ligands, the pathways they activate, as well ...

...-Fast and FLASH TALENs for Endogenous Zebrafish Genes TALENs for Endogenous Human Genes TALENs for the...

...GFP. If there’s GFP expression, presumably the endogenous target site will be cut and repaired by NHEJ ...exhibited a 2-4 fold increase in the number of endogenous indel edits when compared to unsorted cells (...