Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 142 results for: GAL

Showing: 21 - 40 of 142 results

  2. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... system). The GAL80 repressor can bind to GAL4, partially inhibiting the binding of GAL4 to UAS. One can...domain (DBD) to GAL4 or VP16 activation domain results in chimeric GAL80-repressible and GAL80-insensitive...expression is low but is activated by GAL4 binding to UAS. If you place the GAL4 gene downstream of a tissue- ... can therefore place GAL4 and GAL80 under different promoters and create sophisticated patterns of UAS-driven...intermediate expression from QUAS, as seen with GAL4/GAL80 and UAS. QF-mediated repression is reversible...phase of yeast culture. Repressible Binary Systems GAL4/UAS In Drosophila or development studies, you may...across development. One common binary system is the GAL4/UAS system isolated from yeast. In this system, ...

  3. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...virus in cell lines expressing galactosyl(alpha1-3)galactosyl (alphaGal) sugars were less stable than ...and spumaviruses to human serum by galactosyl(alpha1-3)galactosylation. Journal of virology 71:6174–6178...express these sugars. (Takeuchi et al., 1997). The alphaGal sugars end up in the envelope and are targets ...targets for complement-based killing by anti-alphaGal antibodies. The VSV-G envelope protein is inactivated ... such as the one from gibbon ape leukemia virus (GALV) are more stable (Sandrin et al., 2002). Studying...

  4. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...gene expression stable regardless of copy number (Segall-Shapiro et al., 2018). Designing stabilized promoters...constant. In the case of the stabilized promoters from Segall-Shapiro et al., they chose a TALE protein as the...replication, the stabilized promoters created by Segall-Shapiro et al. showed nearly no position-dependent...as cell division slows (Kittleson et al., 2011). Segall-Shapiro et al. showed that the stabilized promoters...number during the transition to stationary phase, Segall-Shapiro et al. placed a trans-acting RepA protein...genes whose proteins are needed sequentially. When Segall-Shapiro et al. placed the genes under the control...these stabilized promoters a try. References 1. Segall-Shapiro, Thomas H., Eduardo D. Sontag, and Christopher...

  5. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...provide legal advice or opinions. The contents of this post should not be relied upon for legal advice ...information presented may not reflect the most current legal developments.  Further, this post may contain technical...attorney should be contacted for advice on specific legal issues.  The permanent residence “green card” process... we recommend that you reach out to experienced legal counsel. Many thanks to our guest bloggers Lauren...Immigration is a federal law, Casseus Law provides legal services to people located in all 50 states and ...

  6. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...s the heart and soul of Addgene’s mission), but legal complexities can arise when scientists are sharing...sharing space, we heard over and over again that the legal documents were one of the biggest hurdles for scientific... of not just the physical samples, but also the legal documentation that allows you to conduct your experiments...easy Even with all those benefits, we know that legal documents can get tricky. Here’s how Addgene helps...

  7. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    ...Drosophila, where expression of Cre from the standard UAS/GAL4 system is toxic to proliferating cells. A Cre-estrogen... recombinase with flies heterozygous for tubulin-GAL4. For Dre, Flp, KD, R, B2 and B3, approximately 50%...50% of offspring carried both the recombinase and GAL4, indicating that the recombinases were inherited...In contrast, 0/79 offspring carried both Cre and GAL4, further emphasizing the toxicity of this enzyme...

  8. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    ...institution’s TTO or legal office first. Generally, Addgene is also waiting to receive that legal agreement. Below... want to wait? Talk with your TTO Has a pending legal agreement ever delayed your access to necessary ...Transfer Office. Some synonymous terms include: legal office, office of sponsored projects/research, office...

  9. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...This post was contributed by Adriana Galvan, an associate professor at Emory University School of Medicine...express hM4Di in neurons of rhesus monkeys or mice (Galvan et al., 2019). The hM4Di receptor is an engineered....     Thank you to our guest blogger! Adriana Galvan is an associate professor at Emory University School...2159–2165 . https://doi.org/10.1128/mcb.8.5.2159 Galvan A, Raper J, Hu X, Paré J, Bonaventura J, Richie...

  10. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ...Td2M6AOpuW pic.twitter.com/ofLI4eD8Zs — Microbigals (@MicrobiGals) December 23, 2020   In second place,...Winning first place in the home edition, the MicrobiGals created glow in the dark petri dish ornaments...

  13. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...experimental model organism. I then described the Gal4/UAS system used by geneticists to study gene function...favorite gene (YFG).  Sometimes, you want to use the Gal4/UAS system, but the available reagents do not match... or by filtering by fly as species of gene. Find Gal4 and UAS plasmids at Addgene Choosing a vector to...

  15. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...genes from the pYES2.1 plasmid is controlled by the GAL1 promoter. The cloning procedure adds both V5 and... to grow in the absence of methionine. Since the GAL1 promoter controls MET/Met gene expression, complementation...complementation should only occur when galactose replaces glucose as the carbon source. Student results have...

  16. Plasmids 101: Protein tags

    Type
    Blog Post
    ...Epitope Mass (kDa) Function Notes CBP KRRWKKNFIAVSAANRFKKISSSGAL 4 Affinity and Purification Binding and...eukaryotes TAP GRRIPGLINP WKRRWKKNFI AVSAANRFKK ISSSGALDYD IPTTASENLY FQGEFGLAQH DEAVDNKFNK EQQNAFYEIL HLPNLNEEQR...PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLAG SGSGHMHHHH HHSSGLVPRG  12 Solubililty...

  18. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...lead to covalent binding of the TMP-tag ligand (Gallagher et al., 2009). HaloTag: Scientists at Promega ...https://doi.org/10.1016/j.chembiol.2019.01.003 Gallagher, S. S., Sable, J. E., Sheetz, M. P., & Cornish...better-dyeing-through-chemistry-small-molecule-fluorophores Los, G. V., Encell, L. P., McDougall, M. G., Hartzell, D. D., Karassina, N., Zimprich...

  19. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ... before.  These attacks on science ultimately galvanized scientists to rally in Ottawa to protest the ...AL, USA 33.5207°N 86.8025°W - Jonathan Aguilar Galileo wrote, “now you would not hide behind these two... large one” (http://oll.libertyfund.org/titles/galilei-dialogues-concerning-two-new-sciences). This is...

  20. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...está disponible en español! Esta traducción fue regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos...materiales de nuestro repositorio. Este increíble regalo permitirá que más personas accedan a recursos útiles...
Showing: 21 - 40 of 142 results