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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 33 results for: HR

Showing: 21 - 33 of 33 results

  3. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...cell viability. Incubate at 30 - 37 °C for 24 - 72 hr. 30 °C may enhance genome editing efficiency, but...each well for a total volume of 200 μl. Wait 24 - 72 hr to allow cells to grow. Aspirate media. Add 50 μl...

  7. CRISPR Plasmids - Tagging

    Type
    Collection
    ...expression cassette for the Cas9-programming sgRNA and HR donors for selectable genome modification. This system...

  9. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...overlapping sequence that direct Homologous Recombination (HR) or Single Strand Annealing (SSA) in the event of...effectively cuts the target sequence, the plasmid undergoes HR or SSA to reconstitute functional GFP, and the cells...

  10. CRISPR References and Information

    Type
    Collection
    ...Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED PDF, 597 KB Zhang gRNA...

  11. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...References and resources References: Bonner WA, Hulett HR, Sweet RG, Herzenberg LA (1972) Fluorescence Activated...

  12. Zhang Lab CRISPR Page

    Type
    Collection
    ...Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, Parnas O, Eisenhaure TM, Jovanovic M...
Showing: 21 - 33 of 33 results