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We narrowed to 25 results for: HR

Showing: 1 - 20 of 25 results

  1. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...have for repairing DNA double strand breaks – (HR). HR is the dominant homology-directed repair (HDR)...will have higher HR frequencies than quiescent cells. It is important to know that HR is often mutated...., eLife)! Use a Cas that facilitates HR What if promoting HR was as simple as swapping out your Cas9...early HR step (Moreno-Mateos, et al., Nat Com). Another method used to prime a Cas break for HR is to...to fuse an HR protein to the nuclease. Early HR factors fused to Cas9 have been shown to increase HDR .... It is recommended you check your cell lines for HR-related mutations before attempting knock-ins by ...Mammalian cells have 2 primary repair mechanisms besides HR - Non-homologous end-joining (NHEJ) and alternative...

  2. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    Published
    Nov. 8, 2016, 3:30 p.m.
    ...screening for DNA markers like the presence of high risk (HR) Human Papillomavirus (HPV). As Gladys wrote in her...cervical cancers are caused by persistent infection of HR HPV types, predominantly HPV 16 and 18.” HPV plasmids... of Ghanaian women by including PCR screening for HR HPV alongside the cytological tests currently in ...will both identify individuals who are infected with HR HPV and therefore at high risk for cervical cancer...

  3. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...your homologous recombination (HR) template, you could then: Confirm your HR by doing Restriction Fragment...genome using CRISPR/Cas forHomologous Recombination (HR)? How long should the homology arms be for efficient.... al, 2013 Science paper). If you detect positive HR, isolate single-cell colonies, grow them up, then... to screen for positive ones.  -- OR -- If your HR template has a selection marker such as puromycin...

  4. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    Published
    March 17, 2021, 2:40 p.m.
    ...She mentioned Addgene was looking for someone with HR expertise to join their newly formed compensation...Harvard, and it let me keep my toe in the water of HR work. What do you think is the most important resource...’s not like I understand the science but my prior HR experience at Harvard Medical School means I understand...

  5. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...overdressed rather than underdressed, but read emails from HR carefully because sometimes companies have relaxed...relaxed dress codes for interviews. You can also ask HR for clarification on the dress code for the interview...video conferencing instructions you received from HR carefully. And even if you don’t have technical challenges...

  6. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...similar spectrum as HR and also results in membrane hyperpolarization but unlike HR, this is achieved through...subsequent firing of an action potential. Halorhodopsins (HR) are green/yellow-light sensitive chloride ion pumps...that Arch may more effectively silence neurons than HR. Delivering your opsin  The standard opsin delivery...

  7. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...break repair instead of homologous recombination (HR). HR is the preferred pathway for generating knock-in...

  9. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...proof-of-principle test, the authors were able to show that HR blocked neuronal activation in the novel context ... Goshen et al, inhibition of the CA1 region using HR was found to block the acquisition and retrieval ...

  14. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...microinjection experiments in S phase zygotes at 18 hr post fertilization. They observed higher targeting...

  15. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...universities may have someone senior from accounts or HR on the judging panel. It’s important for this person...

  16. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...doi.org/10.1128/mcb.5.12.3610-3616.1985 Munro S, Pelham HR (1984) Use of peptide tagging to detect proteins ...

  20. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...procedure to be performed using a simple two-step, 2 hr protocol prior to transformation (Figure 1). As this...
Showing: 1 - 20 of 25 results