We narrowed to 25 results for: HR

...have for repairing DNA double strand breaks – (HR). HR is the dominant homology-directed repair (HDR)...will have higher HR frequencies than quiescent cells. It is important to know that HR is often mutated...., eLife)! Use a Cas that facilitates HR What if promoting HR was as simple as swapping out your Cas9...early HR step (Moreno-Mateos, et al., Nat Com). Another method used to prime a Cas break for HR is to...to fuse an HR protein to the nuclease. Early HR factors fused to Cas9 have been shown to increase HDR .... It is recommended you check your cell lines for HR-related mutations before attempting knock-ins by ...Mammalian cells have 2 primary repair mechanisms besides HR - Non-homologous end-joining (NHEJ) and alternative...

... for using homologous recombination (HR) Q7: Can you summarize HR template vector design guidelines? A7...protospacer target in the HR template, you usually would need to make mutations of the HR template to avoid this... in my HR template an issue? A8: Yes. If you have intact protospacer+PAM sequence within the HR template...your homologous recombination (HR) template, you could then: Confirm your HR by doing Restriction Fragment... few notes below are considerations for designing HR donor. Generally if off-target (non-specific) cleavage...and then select the best guide to co-transfect in HR donor. Alternatively, a new system described recently...cells. One good way is to mutate the PAM 'NGG' in the HR template, like change it to 'NGT', or 'NGA', or '...

...screening for DNA markers like the presence of high risk (HR) Human Papillomavirus (HPV). As Gladys wrote in her...cervical cancers are caused by persistent infection of HR HPV types, predominantly HPV 16 and 18.” HPV plasmids... of Ghanaian women by including PCR screening for HR HPV alongside the cytological tests currently in ...will both identify individuals who are infected with HR HPV and therefore at high risk for cervical cancer...

...She mentioned Addgene was looking for someone with HR expertise to join their newly formed compensation...Harvard, and it let me keep my toe in the water of HR work. What do you think is the most important resource...’s not like I understand the science but my prior HR experience at Harvard Medical School means I understand...

...overdressed rather than underdressed, but read emails from HR carefully because sometimes companies have relaxed...relaxed dress codes for interviews. You can also ask HR for clarification on the dress code for the interview...video conferencing instructions you received from HR carefully. And even if you don’t have technical challenges...

...similar spectrum as HR and also results in membrane hyperpolarization but unlike HR, this is achieved through...subsequent firing of an action potential. Halorhodopsins (HR) are green/yellow-light sensitive chloride ion pumps...that Arch may more effectively silence neurons than HR. Delivering your opsin  The standard opsin delivery...

...break repair instead of homologous recombination (HR). HR is the preferred pathway for generating knock-in...

...difficult-to-manipulate primary cells, achieving knockdown within 1-2 hr of reagent delivery. TRIM-Away is suitable for both...electroporated cells re-adhered to the culture plate within 4 hr of treatment. In some primary cell lines, endogenous...

...proof-of-principle test, the authors were able to show that HR blocked neuronal activation in the novel context ... Goshen et al, inhibition of the CA1 region using HR was found to block the acquisition and retrieval ...

...For example, using a longer growth period (up to 24 hr), reducing the concentration of antibiotics (ie. ...

...submission. If you do not receive such a notice within 24 hrs, send a note to the program coordinator.   6. Virtual...

...increase in mRNA levels after 2 hr and a 400-fold increase after 30 hr. The system was also shown to be...

...microinjection experiments in S phase zygotes at 18 hr post fertilization. They observed higher targeting...

...Ever watchful, but I’m so proud of our culture. HR here works for the Addgenies and I believe they know...

...universities may have someone senior from accounts or HR on the judging panel. It’s important for this person...

...site through error-free homologous recombination (HR). Adapted from an image provided by Manuel Gonçalves...

...Shergill IS, Williamson M, Gommersall L, Arya N, Patel HR. Basic principles of real-time quantitative PCR. ...

...doi.org/10.1128/mcb.5.12.3610-3616.1985 Munro S, Pelham HR (1984) Use of peptide tagging to detect proteins ...

...they just might personally hand your credentials to HR, greatly increasing your chances of getting a phone...

...procedure to be performed using a simple two-step, 2 hr protocol prior to transformation (Figure 1). As this...