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We narrowed to 33 results for: HR

Showing: 1 - 20 of 33 results

  1. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...have for repairing DNA double strand breaks – (HR). HR is the dominant homology-directed repair (HDR)...will have higher HR frequencies than quiescent cells. It is important to know that HR is often mutated...., eLife)! Use a Cas that facilitates HR What if promoting HR was as simple as swapping out your Cas9...early HR step (Moreno-Mateos, et al., Nat Com). Another method used to prime a Cas break for HR is to...to fuse an HR protein to the nuclease. Early HR factors fused to Cas9 have been shown to increase HDR .... It is recommended you check your cell lines for HR-related mutations before attempting knock-ins by ...Mammalian cells have 2 primary repair mechanisms besides HR - Non-homologous end-joining (NHEJ) and alternative...

  2. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    ...screening for DNA markers like the presence of high risk (HR) Human Papillomavirus (HPV). As Gladys wrote in her...cervical cancers are caused by persistent infection of HR HPV types, predominantly HPV 16 and 18.” HPV plasmids... of Ghanaian women by including PCR screening for HR HPV alongside the cytological tests currently in ...will both identify individuals who are infected with HR HPV and therefore at high risk for cervical cancer...

  3. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...your homologous recombination (HR) template, you could then: Confirm your HR by doing Restriction Fragment...genome using CRISPR/Cas forHomologous Recombination (HR)? How long should the homology arms be for efficient.... al, 2013 Science paper). If you detect positive HR, isolate single-cell colonies, grow them up, then... to screen for positive ones.  -- OR -- If your HR template has a selection marker such as puromycin...

  4. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    ...She mentioned Addgene was looking for someone with HR expertise to join their newly formed compensation...Harvard, and it let me keep my toe in the water of HR work. What do you think is the most important resource...’s not like I understand the science but my prior HR experience at Harvard Medical School means I understand...

  5. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...nickase). Homologous Recombination (HR) FAQs Can you summarize HR template vector design guidelines? A...protospacer target in the HR template, you usually would need to make mutations of the HR template to avoid this...sequence) in my HR template an issue? Yes. If you have intact protospacer+PAM sequence within the HR template...your homologous recombination (HR) template, you could then: Confirm your HR by doing Restriction Fragment...more. CRISPR Design FAQs Homologous Recombination (HR) FAQs At the Bench FAQs CRISPR Design FAQs Should... few notes below are considerations for designing HR donor. Generally if off-target (non-specific) cleavage...and then select the best guide to co-transfect in HR donor. Alternatively, a new system described recently...

  6. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...tight. Incubate bacterial culture at 37°C for 12-18 hr in a shaking incubator. Note: Some plasmids or strains...grow for longer periods of time (approximately 18-30 hr). On the other hand, smaller plasmids can be present...number plasmids should only need to be grown for 12-16 hr on average. Certain features of a plasmid may render...

  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... Plasmids mKusabira-Orange (mKO) 548 559 31 5 4.5 hr Monomer pQC mKorange IX - Mammalian Expression mKusabira...- Bacterial Expression mOrange 548 562 49 6.5 2.5 hr Monomer pET mOrange LIC cloning vector (u-mOrange... Bacterial Expression mOrange2 549 565 35 6.5 4.5 hr Monomer mOrange2-N1 - Mammalian Expression mOrange2... - Bacterial Expression tdTomato 554 581 95 4.7 1 hr Tandem-dimer pCSCMV:tdTomato - Mammalian Expression... Structure Plasmids mScarlet-H 551 592 15 4.8 4.4 hr Monomer pmScarlet-H_C1 - Mammalian Expression mNectarine... Mammalian Expression mScarlet 569 594 71 5.3 2.9 hr Monomer pmScarlet_C1 - Mammalian Expression pCS2+...FusionRed-pBAD - Bacterial Expression mRFP1 584 607 13 4.5 1 hr Monomer pcDNA3-mRFP - Mammalian Expression pMXs-mRFP1...

  8. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...overdressed rather than underdressed, but read emails from HR carefully because sometimes companies have relaxed...relaxed dress codes for interviews. You can also ask HR for clarification on the dress code for the interview...video conferencing instructions you received from HR carefully. And even if you don’t have technical challenges...

  9. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...similar spectrum as HR and also results in membrane hyperpolarization but unlike HR, this is achieved through...subsequent firing of an action potential. Halorhodopsins (HR) are green/yellow-light sensitive chloride ion pumps...that Arch may more effectively silence neurons than HR. Delivering your opsin  The standard opsin delivery...

  13. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... Using Cell-Free Protein Synthesis. Oza JP, Aerni HR, Pirman NL, Rogulina S, ter Haar CM, Isaacs FJ, Rinehart...factor 1 deletion. Heinemann IU, Rovner AJ, Aerni HR, Rogulina S, Cheng L, Olds W, Fischer JT, Söll D,...

  19. 6 Tips for Grant Writing

    Type
    Blog Post
    ...universities may have someone senior from accounts or HR on the judging panel. It’s important for this person...
Showing: 1 - 20 of 33 results