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Addgene

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We narrowed to 7 results for: HR

Showing: 1 - 7 of 7 results

  1. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...nickase). Homologous Recombination (HR) FAQs Can you summarize HR template vector design guidelines? A...protospacer target in the HR template, you usually would need to make mutations of the HR template to avoid this...sequence) in my HR template an issue? Yes. If you have intact protospacer+PAM sequence within the HR template...your homologous recombination (HR) template, you could then: Confirm your HR by doing Restriction Fragment...more. CRISPR Design FAQs Homologous Recombination (HR) FAQs At the Bench FAQs CRISPR Design FAQs Should... few notes below are considerations for designing HR donor. Generally if off-target (non-specific) cleavage...and then select the best guide to co-transfect in HR donor. Alternatively, a new system described recently...

  2. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... Plasmids mKusabira-Orange (mKO) 548 559 31 5 4.5 hr Monomer pQC mKorange IX - Mammalian Expression mKusabira...- Bacterial Expression mOrange 548 562 49 6.5 2.5 hr Monomer pET mOrange LIC cloning vector (u-mOrange... Bacterial Expression mOrange2 549 565 35 6.5 4.5 hr Monomer mOrange2-N1 - Mammalian Expression mOrange2... - Bacterial Expression tdTomato 554 581 95 4.7 1 hr Tandem-dimer pCSCMV:tdTomato - Mammalian Expression... Structure Plasmids mScarlet-H 551 592 15 4.8 4.4 hr Monomer pmScarlet-H_C1 - Mammalian Expression mNectarine... Mammalian Expression mScarlet 569 594 71 5.3 2.9 hr Monomer pmScarlet_C1 - Mammalian Expression pCS2+...FusionRed-pBAD - Bacterial Expression mRFP1 584 607 13 4.5 1 hr Monomer pcDNA3-mRFP - Mammalian Expression pMXs-mRFP1...

  3. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... Using Cell-Free Protein Synthesis. Oza JP, Aerni HR, Pirman NL, Rogulina S, ter Haar CM, Isaacs FJ, Rinehart...factor 1 deletion. Heinemann IU, Rovner AJ, Aerni HR, Rogulina S, Cheng L, Olds W, Fischer JT, Söll D,...

  4. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...cell viability. Incubate at 30 - 37 °C for 24 - 72 hr. 30 °C may enhance genome editing efficiency, but...each well for a total volume of 200 μl. Wait 24 - 72 hr to allow cells to grow. Aspirate media. Add 50 μl...

  5. CRISPR Plasmids - Tagging

    Type
    Collection
    ...expression cassette for the Cas9-programming sgRNA and HR donors for selectable genome modification. This system...

  6. CRISPR References and Information

    Type
    Collection
    ...Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED PDF, 597 KB Zhang gRNA...

  7. Zhang Lab CRISPR Page

    Type
    Collection
    ...Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, Parnas O, Eisenhaure TM, Jovanovic M...
Showing: 1 - 7 of 7 results