We narrowed to 57 results for: Kras

1. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

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   Blog Post

   ...we are now adopting dCas9, dCas9-VP64, and dCas9-KRAB in our system,” Sakuma said. “These are sure to ...

2. What's New in CRISPR - March 2020

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   Blog Post

   ...AAVS1-integrated, inducible, and fluorescent dCas9-KRAB and dCas9-VPR for quantifying and tracking cells...

3. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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   Blog Post

   ...HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt H, Krause MW, Seydoux G. Genetics. 2014 Dec;198(4):1347-56...

4. Plasmids 101: The protein expression toolbox

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   Blog Post

   ...repression of protein expression. Addgene has a dCas9 KRAB plasmid for transient knockouts!  Turn it on Turn...

5. CRISPR 101: Cas9 vs. The Other Cas(s)

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   ... (2018). 10.1126/science.aav4294 Ozcan, Ahsen, Krajeski, Rohan, Ioannidi, Eleonara, et al., Programmable...

6. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   Blog Post

   ...Edraki A, Garcia B, Amrani N, Chen JS, Cofsky JC, Kranzusch PJ, Sontheimer EJ, Davidson AR, Maxwell KL, Doudna...

7. Making CRISPR Plasmids Using Fragmid

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   Blog Post

   ...V., Griffith, A. L., Szegletes, Z. M., Wen, B., Kraus, C., Miller, N. W., Steger, R. J., Escude Velasco...

8. Viral Vectors 101: Calcium Sensors

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   Blog Post

   ...References and Resources References Nasu Y, Shen Y, Kramer L, Campbell RE. Structure-and mechanism-guided ...

9. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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   ..., B., Morikawa, R.K., Rompani, S.B., Hantz, P., Szikra, T., Esposti, F., Cowan, C.S., Bharioke, A., Patino-Alvarez...

10. An Introduction to Adenovirus

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    ...6936.1999. PMID: 10400791. Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & Verma, I. (1998...

11. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    Blog Post

    ...is then tethered to a repressive domain, such as KRAB, to prevent efficient transcription from sgRNA targets...

12. Hot Plasmids: Summer 2024

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    Blog Post

    ...10.1038/s41587-021-01133-w Pandey, S., Gao, X. D., Krasnow, N. A., McElroy, A., Tao, Y. A., Duby, J. E., Steinbeck...

13. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    Blog Post

    ... Lee C-YS, Calidas D, Lu T, Smith J, Schmidt H, Krause MW, Seydoux G (2014) Scalable and Versatile Genome...

14. Typing CRISPR Systems

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    Blog Post

    ...://doi.org/10.1089/crispr.2021.0051 Özcan, A., Krajeski, R., Ioannidi, E., Lee, B., Gardner, A., Makarova...

15. CRISPR 101: Cytosine and Adenine Base Editors

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    Blog Post

    ...01611-9 Neugebauer, M. E., Hsu, A., Arbab, M., Krasnow, N. A., McElroy, A. N., Pandey, S., Doman, J. L...

16. Lentivirus Production

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    Protocol

    ...cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would be...

17. Colony Formation Titering Assay

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    Protocol

    ...dilutions of the lentiviral vector pHAGE EF1α dCas9-KRAB . The following day, cells were treated with puromycin...

18. 28 Hot Plasmid Technologies from 2015

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    Blog Post

    ...Addgene. Hochbaum, et al. Nat Methods. 2014. Kralj, et al. Nat Methods. 2011. High-throughput luciferase...

19. Immunology Research Plasmids and Resources

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    ...SIP-145, hp51CN JUN jun oncogene AP-1, AP1, c-Jun KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog...D12S2489E, FLJ17759, FLJ75772, KLR, NKG2-D, NKG2D KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog...MGC126258, PSCTK2 JUN jun oncogene AP-1, AP1, c-Jun KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog... K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS3, RASK2 LILRB3 leukocyte immunoglobulin-... K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS3, RASK2 LAT linker for activation of T cells... factor X-associated ankyrin-containing protein ANKRA1, BLS, F14150_1, MGC138628, RFX-B RFXAP regulatory...

20. Tetracycline Inducible Expression

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    ...167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G Tet-inducible expression of KRAB-dCas9-DHFR for CRISPRi...Tet-On 3G rtTA TRE3GS Jason Sheltzer 11651 pLVUT-tTR-KRAB Lentiviral Tet-On vector for inducible expression...2006 ) for Tet-Off and additional plasmids. tetR-KRAB TRE Patrick Aebischer , Didier Trono 41393 pCW57.1...