We narrowed to 144 results for: LYC

...target protein, so they are referred to as polyclonal. Polyclonal antibodies have the advantage of more robustly...find more details about polyclonal antibodies on our Antibodies 101: Polyclonal Antibodies blog post. Monoclonal...advantages and disadvantages compared to each other. Polyclonal antibodies These antibodies are derived from ...

...Action*   Working Concentration** Kanamycin aminoglycoside Binds 30S ribosomal subunit; causes mis-translation...mis-translation Bactericidal 50-100 ug/mL Spectinomycin aminoglycoside Binds 30S ribosomal subunit; interrupts protein... Bactericidal 7.5-50 ug/mL Streptomycin aminoglycoside Inhibits initiation of protein synthesis ...synthesis Bactericidal 100 ug/mL Bleomycin glycopeptide Induces DNA breaks Bactericidal 5-100 ug/mL...

... be shared across other proteins. Conversely, polyclonal antibodies - which are a heterogenous mixture...antibodies, their cost is higher than that of polyclonal antibodies. For example, it takes longer to develop...than it does to immunize an animal and recover polyclonal antibodies (Lipman et al., 2005). This is reflected...Trudel LJ, Weis-Garcia F (2005) Monoclonal Versus Polyclonal Antibodies: Distinguishing Characteristics, Applications...

... Traditionally, they have been made as either polyclonal antibodies or monoclonal hybridomas, but those...those techniques have several drawbacks. Polyclonal antibodies produced in animals may be variable among...& Plückthun, 2015a).   Figure 1: (Top) Polyclonal antibodies are produced in animals and consist...Recombinant antibodies offer numerous advantages over polyclonal antibodies and traditional hybridomas.  First...

...create your own glycerol stocks for more frequent use. This way, if one of your new glycerol stocks gets ... arrive in your laboratory as plates of frozen glycerol stocks. It is very important that these stay frozen...and preferably on dry ice. Careful use of your glycerol stocks When your lab adopts the MoClo technology...

...cloning capacity of ~8 kb, so either a single polycistronic vector or multiple single-gene vectors can be...retroviral vectors. This makes it possible to use a polycistronic cassette that expresses multiple reprogramming...factors can be delivered either as a single polycistronic transgene or with four different adenoviruses... ~14.5kb minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG...

... CRISPR knockout library Article contributed by Alycia Bittner CRISPR pooled libraries are popular tools...ApoB-LPs) are indirectly measured through their triglyceride or cholesterol contents (what is commonly called...and indirect measurement of ApoB-LPs through triglyceride/cholesterol does not provide a clear view of...

...targets human glycosyltransferase genes and other proteins related to cellular glycosylation. Get the library... PAM. Get the plasmids at Addgene The Human GlycoGene CRISPR Library is a new lentiviral CRISPR library...

...protein folding, formation of disulfide bridges, glycosylation and acetylation to create functional, stable...systems enable high expression of very complex, glycosylated proteins that cannot be produced in  E. coli...optimized. Yeast cells are also known for hyperglycosylating proteins which may be an issue depending ...

... Addgene - DNA sent from depositors, bacterial glycerol stocks, prepped DNA ready for our quality control...storage on Thursday. The lab prepares bacterial glycerol stocks on Fridays. From each overnight culture... to transformed cells and finally to bacterial glycerol stocks that are used to fulfill orders.  The barcoded...

...EAAC1 Rat Mouse IgG2a 206573 Anti-Glycine receptor Alpha3 [N424/45R] Glycine receptor Alpha3 Human Mouse IgG2a... IgG2a 206574 Anti-Glycine receptor Alpha3L [N424/48R] Glycine receptor Alpha3L Human Mouse IgG2a 206575...receptor Mouse Mouse 206774 Glycine receptor Alpha3 scFv [N424/45] N424/45 scFv Glycine receptor Alpha3 Human...

...repair mediated by uracil glycosylase (Figure 1). The presence of a uracil glycosylase inhibitor (UGI) minimizes...promoter driven expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple...

...positively charged polycation, polybrene. Interestingly, one study found that alternative polycations such as DEAE-dextran...efficiency of lentiviral vectors: effect of sera and polycations.” Molecular Biotechnology 53.3 (2013). 308-14...

...synthesis, and increases energy production through glycolysis to fuel these energy-intensive processes. mTORC1...associated protein; LST8 homolog GSK3 GSK3A GSK3B Glycogen synthase kinase 3 MDM2 MDM2 proto-oncogene mSin1...

...dUTPase and uracil deglycosidase. dUTPase prevents the buildup of dUTP and uracil deglycosidase removes uracil...uracil-labeled template strand is degraded by uracil DNA glycosylase (UDG) and Exonuclease III (ExoIII) (step 4). ...

...structure. Glypicans are proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG), and are (typically...

...or repair. Fusing an inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair,...producing a double strand break. UGI = uracil DNA glycosylase. Created with BioRender.com. The BE3 system...byproducts likely resulted from excision by uracil N-glycosylase (UNG) during base excision repair. Adding a second...

...distribution funds our operation. Antibodies are glycoproteins that are part of the humoral immune system. ...against a target antigen. This contrasts with polyclonal antibodies, which are mixtures of monoclonal ...

...biorender.com.    Since antibodies, especially polyclonal antibodies, can vary lot-to-lot, this range needs...this starting point. You can also consider using polyclonal or recombinant multiclonal antibodies to increase... time Not enough antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein...

...as glyphosate, in water. He aims to leverage a glycine oxidase reaction to indirectly detect glyphosate...will co-express together with a plasmid encoding glycine oxidase. The goal is to determine potential water...