Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 146 results for: LYC

Showing: 21 - 40 of 146 results

  1. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... be shared across other proteins. Conversely, polyclonal antibodies - which are a heterogenous mixture...antibodies, their cost is higher than that of polyclonal antibodies. For example, it takes longer to develop...than it does to immunize an animal and recover polyclonal antibodies (Lipman et al., 2005). This is reflected...Trudel LJ, Weis-Garcia F (2005) Monoclonal Versus Polyclonal Antibodies: Distinguishing Characteristics, Applications...

  2. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    ... Traditionally, they have been made as either polyclonal antibodies or monoclonal hybridomas, but those...those techniques have several drawbacks. Polyclonal antibodies produced in animals may be variable among...& Plückthun, 2015a).   Figure 1: (Top) Polyclonal antibodies are produced in animals and consist...Recombinant antibodies offer numerous advantages over polyclonal antibodies and traditional hybridomas.  First...

  3. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...create your own glycerol stocks for more frequent use. This way, if one of your new glycerol stocks gets ... arrive in your laboratory as plates of frozen glycerol stocks. It is very important that these stay frozen...and preferably on dry ice. Careful use of your glycerol stocks When your lab adopts the MoClo technology...

  4. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...cloning capacity of ~8 kb, so either a single polycistronic vector or multiple single-gene vectors can be...retroviral vectors. This makes it possible to use a polycistronic cassette that expresses multiple reprogramming...factors can be delivered either as a single polycistronic transgene or with four different adenoviruses... ~14.5kb minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG...

  5. Immunology Research Plasmids and Resources

    Type
    Collection
    ...MGC149868, hCS-L CSPG5 chondroitin sulfate proteoglycan 5 (neuroglycan C) MGC44034, NGC CTF1 cardiotrophin 1...MGC99678 GPHA2 glycoprotein hormone alpha 2 A2, GPA2, MGC126572, ZSIG51 GPHB5 glycoprotein hormone beta ...subunit ADTB3, ADTB3A, HPS, HPS2, PE AZGP1 alpha-2-glycoprotein 1, zinc-binding ZA2G, ZAG B2M beta-2-microglobulin...osteosarcoma viral oncogene homolog AP-1, C-FOS GSK3B glycogen synthase kinase 3 beta - HRAS v-Ha-ras Harvey ...laevis) DAND4, MGC119894, MGC119895, MGC96951 CGA glycoprotein hormones, alpha polypeptide CG-ALPHA, FSHA, ...MIF macrophage migration inhibitory factor (glycosylation-inhibiting factor) GIF, GLIF, MMIF MLN motilin...gfg-1 OGFR opioid growth factor receptor - OGN osteoglycin DKFZp586P2421, OG, OIF, SLRR3A OPRD1 opioid receptor...

  7. Hot Plasmids - October 2020

    Type
    Blog Post
    ...targets human glycosyltransferase genes and other proteins related to cellular glycosylation. Get the library... PAM. Get the plasmids at Addgene The Human GlycoGene CRISPR Library is a new lentiviral CRISPR library...

  8. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...protein folding, formation of disulfide bridges, glycosylation and acetylation to create functional, stable...systems enable high expression of very complex, glycosylated proteins that cannot be produced in  E. coli...optimized. Yeast cells are also known for hyperglycosylating proteins which may be an issue depending ...

  9. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ... Addgene - DNA sent from depositors, bacterial glycerol stocks, prepped DNA ready for our quality control...storage on Thursday. The lab prepares bacterial glycerol stocks on Fridays. From each overnight culture... to transformed cells and finally to bacterial glycerol stocks that are used to fulfill orders.  The barcoded...

  10. Open Enzyme Collection

    Type
    Collection
    ...pOpen-UDG UDG Uracil DNA glycosylase 165541 pOpen-Fpg Fpg Formamidopyrimidine DNA glycosylase 165550 pOpen-T4 ...pOpen-T4 PGD T4 PGD (T4 pyrimidine DNA glycosylase, aka T4 Endonuclease V) 165540 pOpen-T7EI T7 Endonuclease ...

  11. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...positively charged polycation, polybrene. Interestingly, one study found that alternative polycations such as DEAE-dextran...efficiency of lentiviral vectors: effect of sera and polycations.” Molecular Biotechnology 53.3 (2013). 308-14...

  12. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...repair mediated by uracil glycosylase (Figure 1). The presence of a uracil glycosylase inhibitor (UGI) minimizes...promoter driven expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple...

  13. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...dUTPase and uracil deglycosidase. dUTPase prevents the buildup of dUTP and uracil deglycosidase removes uracil...uracil-labeled template strand is degraded by uracil DNA glycosylase (UDG) and Exonuclease III (ExoIII) (step 4). ...

  14. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...structure. Glypicans are proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG), and are (typically...

  15. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...or repair. Fusing an inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair,...producing a double strand break. UGI = uracil DNA glycosylase. Created with BioRender.com. The BE3 system...byproducts likely resulted from excision by uracil N-glycosylase (UNG) during base excision repair. Adding a second...

  17. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...biorender.com.    Since antibodies, especially polyclonal antibodies, can vary lot-to-lot, this range needs...this starting point. You can also consider using polyclonal or recombinant multiclonal antibodies to increase... time Not enough antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein...

  18. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... single vector. sgRNAs are expressed as polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG cassettes...Gateway recombination-based cloning of complex polycistronic lentiviruses. Can express Cas9 with up to 3 ...

  19. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...EAAC1 Rat Mouse IgG2a 206573 Anti-Glycine receptor Alpha3 [N424/45R] Glycine receptor Alpha3 Human Mouse IgG2a... IgG2a 206574 Anti-Glycine receptor Alpha3L [N424/48R] Glycine receptor Alpha3L Human Mouse IgG2a 206575...receptor Mouse Mouse 206774 Glycine receptor Alpha3 scFv [N424/45] N424/45 scFv Glycine receptor Alpha3 Human...
Showing: 21 - 40 of 146 results