We narrowed to 532 results for: PAN
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TypeCollection...Knockout Library 138015 Knockout Mouse Pu N/A 6 14,671 Pan-cancer CasRx lncRNA Albarossa Library 212966 Knockout... 64750 Knockout D. melanogaster Liu N/A 3 40,279 Pan-Druggable Cancer Library 182133 Knockout Human Mali...
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Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection....0-minBG-SYFP2-WPRE3-BGHpA AiP11390 DLX2.0 SYFP2 Pan GABAergic interneurons Isocortex 222939 pAAV-DLX2.0...pAAV-DLX2.0-minBG-FlpO-WPRE-HGHpA AiP14532 DLX2.0 FlpO Pan GABAergic interneurons Isocortex 214518 pAAV-AiE2366m-minBG-SYFP2... -
Zebrafish Plasmid Collection
TypeCollection...cell tracing - Sean Megason Lab Zebrabow - Albert Pan and Alex Schier Labs. Multicolor fluorescent labeling...during Development and Regeneration - Periklis Pantazis Lab Multibow: digital spectral barcodes for cell... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog Post...Cancer Mutant Collection) or “pan-cancer” analyses across many cancer types (Pan Cancer Mutant Collection)...Cancer Discovery 2016. PubMed PMID: 27147599. The pan cancer mutant collection and the genetic pathway ... -
CRISPR Activation: A Practical Guide
TypeBlog Post...gain insight into protein structure and function (Pan et al., 2018). cDNA libraries, which consist of plasmids...Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman...517:583–588 . https://doi.org/10.1038/nature14136 Pan J, Meyers RM, Michel BC, Mashtalir N, Sizemore AE... -
Hot Plasmids: Winter 2025
TypeBlog Post...Kv. C) Cultured rat hippocampal neuron expressing pan-membrane ASAP5. Solid blue circle marks the initiation... soma via the Kv2.1 targeting motif as well as a pan-membrane construct without the Kv motif. Altogether...and the soma. The initiation site identified in panel (C) is indicated; red line is exponential fit. Adapted... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...with Zebrabow Before the generation of Zebrabow (Pan et al., 2011), Brainbow was also used in zebrafish...Nature 450.7166 (2007): 56. PubMed PMID: 17972876. Pan, Y. Albert, et al. "Multicolor Brainbow imaging in...cutaneous melanoma induced by mutant BRaf arises from expansion and dedifferentiation of mature pigmented melanocytes... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post...different isotypes. (A) Neocortex labeled with anti-pan-Nav (magenta), anti-CASPR (green), and anti-Kv2.1...Secondary antibody SCN1A sodium channels anti-pan-Nav R-mAb K58/35R (Mouse IgG2a) Goat anti-mouse...anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail of the box in C. Image...). Even better, show each channel in individual panels, then the multicolor overlay; this helps all viewers... -
CRISPR History and Development for Genome Engineering
TypeCollection...Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman...rationally designed mutations. Researchers have expanded the CRISPR field beyond SpCas9, moving us closer...and translational technologies. Biotechnology companies are exploring therapeutic applications of CRISPR... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...resistance, Bhang says “the [drug] resistance story panned out quickly,” and was summarized in a recent Nature... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...for neuron-specific expression (hSyn promoter) and pan-mammalian cell expression (CAG promoter). An improved... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post... for your experiment. If you’re looking for more pan-genome PAM flexibility, you’ll want to consider one...., et al.,Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science 361,1259-1262(2018). ... on the Addgene blog The PAM Requirement and Expanding Beyond Cas9 xCas9: Engineering a CRISPR Variant... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog Post.... PubMed PMID: 18845710. Kim, Dal Hyung, et al. "Pan-neuronal calcium imaging with cellular resolution... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal) promoters (Figure 1b), and sgRNAs can be... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...10.1074/jbc.TM118.000372 Yang, H., Ren, S., Yu, S., Pan, H., et al. Methods favoring homology-directed repair... -
The AAVantages of AAV in CRISPR Screens
TypeBlog Post....2022.09.015 Ramani, B., Rose, I. V., Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal, T., Yadanar, L., ... -
An Introduction to Adenovirus
TypeBlog Post...Lovett, S. P., Parker, K., Radune, D., Hatada, A., Pan, C. Y., Rizzo, K., Bertumen, J. B., Morales, C., ... -
Plasmids 101: Biotinylation
TypeBlog Post...125.27 (2003): 8074–8075. PubMed PMID:12837056. 8. Pan, J. feng, Liu, N. hua, Shu, L. yuan, & Sun, H. “Application... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog Post...Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020...part of the world manifesting as a full-fledged pandemic. Containing the spread of this virus has become...widespread testing is crucial in controlling this pandemic. Currently, the CDC uses RT-qPCR tests to diagnose...