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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 532 results for: PAN

Showing: 21 - 40 of 532 results

  1. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ... a startup company. However, like many like-minded people, I quickly realize that a company requires more...during graduate school and turn it into a biotech company. Transitioning an idea from academia to a startup...not remove himself from the idea of starting a company around engineered bacteriophages. He decided to...Development & Operations. Starting a biotech company When and how does a startup become "successful...cornerstone of it all. Without a good nucleus, the company won’t go anywhere. The team should initially consist...conversation about its goals and vision for the company; this will help avoid future complications and ...that waste money and time. Most importantly, “the company does not become real until someone takes the risk...

  5. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...gain insight into protein structure and function (Pan et al., 2018). cDNA libraries, which consist of plasmids...Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman...517:583–588 . https://doi.org/10.1038/nature14136 Pan J, Meyers RM, Michel BC, Mashtalir N, Sizemore AE...

  6. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...Kv. C) Cultured rat hippocampal neuron expressing pan-membrane ASAP5. Solid blue circle marks the initiation... soma via the Kv2.1 targeting motif as well as a pan-membrane construct without the Kv motif. Altogether...and the soma. The initiation site identified in panel (C) is indicated; red line is exponential fit. Adapted...

  7. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman...rationally designed mutations. Researchers have expanded the CRISPR field beyond SpCas9, moving us closer...and translational technologies. Biotechnology companies are exploring therapeutic applications of CRISPR...

  8. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...with Zebrabow Before the generation of Zebrabow (Pan et al., 2011), Brainbow was also used in zebrafish...Nature 450.7166 (2007): 56. PubMed PMID: 17972876. Pan, Y. Albert, et al. "Multicolor Brainbow imaging in...cutaneous melanoma induced by mutant BRaf arises from expansion and dedifferentiation of mature pigmented melanocytes...

  9. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...different isotypes. (A) Neocortex labeled with anti-pan-Nav (magenta), anti-CASPR (green), and anti-Kv2.1...Secondary antibody SCN1A sodium channels anti-pan-Nav R-mAb K58/35R (Mouse IgG2a) Goat anti-mouse...anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail of the box in C. Image...). Even better, show each channel in individual panels, then the multicolor overlay; this helps all viewers...

  10. Plan Your Experiment

    Type
    Collection
    ...E., Chen, Y., Whitehead, E. H., Guimaraes, C., Panning, B., Ploegh, H. L., Bassik, M. C., Qi, L. S., Kampmann...relative ease. Over the past decade, researchers have expanded the CRISPR toolbox to include many different types...target in your samples to ensure there are no discrepancies with the reference sequence you used for gRNA...

  14. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ... for your experiment. If you’re looking for more pan-genome PAM flexibility, you’ll want to consider one...., et al.,Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science 361,1259-1262(2018). ... on the Addgene blog The PAM Requirement and Expanding Beyond Cas9 xCas9: Engineering a CRISPR Variant...

  19. An Introduction to Adenovirus

    Type
    Blog Post
    ...Lovett, S. P., Parker, K., Radune, D., Hatada, A., Pan, C. Y., Rizzo, K., Bertumen, J. B., Morales, C., ...
Showing: 21 - 40 of 532 results