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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 58 results for: PSI-P

Showing: 21 - 40 of 58 results

  1. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Larsen R, Gore BB, Szelenyi ER, Trader C, Balaram P, van Velthoven CTJ, Chiang M, Mich JK, Dee N, Goldy... NV, Martinez RA, Fong O, Yao S, Mortrud M, Chong P, Loftus L, Bertagnolli D, Goldy J, Casper T, Dee N...BRAIN Armamentarium Collection Caltech Systemic Capsids AAV Packaged on Request AAV Guide Plasmids Publications...

  2. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...research   Optimized prime editing in dicots By Emily P. Bentley Prime editing (PE), initially developed in... H., Natale, S., Gomez, L., Taxidis, J., O'Neill, P. S., Villette, V., Bradley, J., Wang, Z., Jiang, D...PE2max-NC and ePEmax3 optimized for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right... to drive prime editing in both tomatoes and Arabidopsis, suggesting the improvements may be broadly applicable...enables heritable desired edits in tomato and Arabidopsis. Nature Plants, 10(10), 1502–1513. doi: https...

  3. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    .... https://doi.org/10.1038/nature02426 Iannaccone, P. M., & Jacob, H. J. (2009). Rats! Disease Models &...Liu, L. S., Zhao, L. Y., Wang, S. H., & Jiang, J. P. (2016). Research proceedings on amphibian model organisms...is as a biomedical model organism.  Arabidopsis thaliana Arabidopsis thaliana is one of, if not the most...community.  The Arabidopsis genome project marked the beginning of widespread Arabidopsis genetics research...characterized genome, and widespread utilization, Arabidopsis has made significant contributions to understanding...understanding plant and crop science alike.  Arabidopsis has many of the traits that make an ideal model organism...maintained in a laboratory environment. What sets Arabidopsis apart from other plant model organisms is its...

  4. Common Injection Routes in Mice

    Type
    Blog Post
    ... plasmids Cre-Lox plasmids References Prathipati, P., Rodriguez-Aguayo, C., Walton, B. L., Sood, A. K.....), The Laboratory Mouse. Academic Press. Turner, P. V., Brabb, T., Pekow, C., & Vasbinder, M. A. (2011... place the needle in the correct vein without collapsing it. Retro-orbital injections are also thought...

  5. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... ER-negative HEK293 cells." PLoS One, 2014. 9(6): p. e98635. PubMed PMID: 24901218. PubMed Central PMCID...2015): 2321-2332. 3. Contamination, Ileana S., John P. S., Basic Methods, Human Stem Cell Manual (Second...PMCID: PMC4047077. 2. Pagliara, Patrizia, et al. "Ostreopsis cf. ovata induces cytoskeletal disorganization...

  6. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ..., Public Library of Science (PLoS), 18 Feb. 2011, p. e16765. Crossref, https://doi.org/10.1371/journal.pone...5, Public Library of Science (PLoS), 30 May 2018, p. e0197185. Crossref, https://doi.org/10.1371/journal.pone...Communications, vol. 2, no. 2, Elsevier BV, Mar. 2021, p. 100135. Crossref, https://doi.org/10.1016/j.xplc.2020.100135...Generation of 8× Nicotiana Benthamiana and 12× Arabidopsis Mutants.” The Plant Journal, vol. 106, no. 1,...

  7. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...Boye, S. E., & Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve Transduction and Volumetric...Hurtrel, B., Heard, J.-M., Rivière, Y., & Moullier, P. (2002). Lack of an Immune Response against the Tetracycline-Dependent...refers to the different protein compositions of AAV capsids, which determine how a virus enters the cell. Different...Selective promoters Selective promoters, such as synapsin, glial fibrillary associated protein (GFAP), or...

  8. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...subdivide neural expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature...with combinatorial labels. Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet ...schematic at the bottom of this post for a basic synopsis of the differences amongst the Brainbow systems...

  9. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... Bohn, P., Joines, K.M., Peng, H., Hawrylycz, M.J., Phillips, J.W., Hohmann, J.G., Wohnoutka, P., Gerfen....L., Kieran, D., Redmond, D.E.Jr., and Aebischer, P. (2010). Efficient transduction of non-human primate... like lentivirus, but instead is contained by a capsid which is comprised of the viral proteins VP1, VP2...genetically engineered to have differences in their capsid proteins. For a brief overview see Addgene’s AAV...

  10. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ....3389/fmed.2023.1106085. PMID: 36817775. Wilmott, P., Lisowski, L., Alexander, I. E., & Logan, G. J. (...conjunction with the viral rep (replication) and cap (capsid) proteins, which bind to the ITRs and initiate ...containing the AAV2 ITRs can be cross-packaged with capsids from multiple AAV serotypes (Wilmott et al., 2019...2019), and different combinations of ITR and capsid serotypes vary in their gene transfer efficiency. ... 101: Pseudotyping Viral Vectors 101: Systemic Capsids More resource on Addgene.org Addgene's AVV guide...2023). AAV- based vector improvements unrelated to capsid protein modification. Front Med, 10, 1106085. https...

  11. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...doi.org/10.1038/s41477-020-0704-5 Santana I, Wu H, Hu P, Giraldo JP (2020) Targeted delivery of nanomaterials...doi.org/10.1038/s41467-020-15731-w Wang F, Wang C, Liu P, Lei C, Hao W, Gao Y, Liu Y-G, Zhao K (2016) Enhanced...including commonly used model organisms like Arabidopsis and Medicago truncatula and several crop species...

  12. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...loci in human cells. Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci...chromosomal translocations or chromosomal shattering (chromothripsis). As this study joins the pantheon of useful...

  13. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ... https://doi.org/10.3389/fnana.2019.00093 Wilmott P, Lisowski L, Alexander IE, Logan GJ (2019) A User’...very high, ubiquitous expression, while the human synapsin 1 promoter is neuronal specific (Haery et al.,...

  14. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...Thank you to our guest blogger Derek Simon! Derek P. Simon received his PhD in Cellular and Molecular ... appropriate wavelength to stimulate your opsin. Each opsin has an excitation spectrum (use the resources...materials science-based. Light is delivered to your opsin through a small piece of fiber optic cable implanted...stimulation guarantees that the majority of the opsins will receive sufficient quantities of photons to... otherwise optical stimulation will be erratic, opsin activation/inactivation inconsistent, and behavioral...stimulation         In the video above, Channelrhodopsin 2 (Chr2) has been expressed in the motor cortex...laser must be controlled. As discussed above, each opsin variant has slightly different activation/inactivation...

  15. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Christiaen p(U6a-BsaI-gRNA) 65955 Other/Tribolium castaneum BsaI none S. pyogenes Averof p(U6b-BsaI-gRNA...Bacteria BsaI yes, activate S. pyogenes Marraffini pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) 47868 Mammalian...

  16. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...Gross-Scherf, B., Morikawa, R.K., Rompani, S.B., Hantz, P., Szikra, T., Esposti, F., Cowan, C.S., Bharioke, ...focused on modifying the outside protein shell, or capsid, of the virus so that it only enters and delivers...the same transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-...

  17. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...v3060677 Varanda C, Félix MDR, Campos MD, Materatski P. An Overview of the Application of Viruses to Biotechnology...serotypes (12 naturally occurring and more synthetic capsids are routinely being developed) to choose from, ...

  18. Viral Production at Addgene

    Type
    Blog Post
    ... element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for normalization...intensity of the viral capsid proteins are analyzed. The abundance of viral capsid proteins as a fraction...the relative stoichiometric ratios of the viral capsid proteins VP1, VP2, and VP3. Samples of viral preparations...non-purified AAV subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are shown relative to...

  19. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...Concepcion CP, Bonetti C, Vidigal JA, Han Y-C, Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura...other vectors (Lukashev et al., 2016). The protein capsid encapsulating the genome is majorly composed of...Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far) or for cancer...

  20. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ... F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck NB. A directional strategy for monitoring...and Sternson SM. 2008. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range...
Showing: 21 - 40 of 58 results