...Primers and a probe against the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) are used as a control for...cations can affect the attachment of adherent cells) Trypsin EDTA (TrypLE, Thermo Fisher, 12605010) Ethanol,...

...research   Optimized prime editing in dicots By Emily P. Bentley Prime editing (PE), initially developed in... H., Natale, S., Gomez, L., Taxidis, J., O'Neill, P. S., Villette, V., Bradley, J., Wang, Z., Jiang, D...PE2max-NC and ePEmax3 optimized for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right... to drive prime editing in both tomatoes and Arabidopsis, suggesting the improvements may be broadly applicable...enables heritable desired edits in tomato and Arabidopsis. Nature Plants, 10(10), 1502–1513. doi: https...

...CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) pathway, as an RNA-guided DNA...activation domain and tagBFP 46914 pU6-sgGFP-NT1 Human pSico-based U6 vector containing murine U6 promoter and...sgRNA targeting GFP (NT1) 46917 pU6-sgCXCR4-2 Human pSico-based U6 vector containing murine U6 promoter and...sequence of human MUC4 exon 3 46915 pU6-sgGAL4-1 Human pSico-based U6 vector containing murine U6 promoter and...targeting GAL4 UAS promoter 46916 pU6-sgGAL4-4 Human pSico-based U6 vector containing murine U6 promoter and...promoter (negative control) 46918 pU6-sgCD71-2 Human pSico-based U6 vector containing murine U6 promoter and...

.... https://doi.org/10.1038/nature02426 Iannaccone, P. M., & Jacob, H. J. (2009). Rats! Disease Models &...Liu, L. S., Zhao, L. Y., Wang, S. H., & Jiang, J. P. (2016). Research proceedings on amphibian model organisms...is as a biomedical model organism.  Arabidopsis thaliana Arabidopsis thaliana is one of, if not the most...community.  The Arabidopsis genome project marked the beginning of widespread Arabidopsis genetics research...characterized genome, and widespread utilization, Arabidopsis has made significant contributions to understanding...understanding plant and crop science alike.  Arabidopsis has many of the traits that make an ideal model organism...maintained in a laboratory environment. What sets Arabidopsis apart from other plant model organisms is its...

... ER-negative HEK293 cells." PLoS One, 2014. 9(6): p. e98635. PubMed PMID: 24901218. PubMed Central PMCID...2015): 2321-2332. 3. Contamination, Ileana S., John P. S., Basic Methods, Human Stem Cell Manual (Second...PMCID: PMC4047077. 2. Pagliara, Patrizia, et al. "Ostreopsis cf. ovata induces cytoskeletal disorganization...

... plasmids Cre-Lox plasmids References Prathipati, P., Rodriguez-Aguayo, C., Walton, B. L., Sood, A. K.....), The Laboratory Mouse. Academic Press. Turner, P. V., Brabb, T., Pekow, C., & Vasbinder, M. A. (2011... place the needle in the correct vein without collapsing it. Retro-orbital injections are also thought...

... element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for normalization...intensity of the viral capsid proteins are analyzed. The abundance of viral capsid proteins as a fraction...the relative stoichiometric ratios of the viral capsid proteins VP1, VP2 and VP3. Samples of viral preparations...non-purified AAV subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are shown relative to...

..., Public Library of Science (PLoS), 18 Feb. 2011, p. e16765. Crossref, https://doi.org/10.1371/journal.pone...5, Public Library of Science (PLoS), 30 May 2018, p. e0197185. Crossref, https://doi.org/10.1371/journal.pone...Communications, vol. 2, no. 2, Elsevier BV, Mar. 2021, p. 100135. Crossref, https://doi.org/10.1016/j.xplc.2020.100135...Generation of 8× Nicotiana Benthamiana and 12× Arabidopsis Mutants.” The Plant Journal, vol. 106, no. 1,...

...Larsen R, Gore BB, Szelenyi ER, Trader C, Balaram P, van Velthoven CTJ, Chiang M, Mich JK, Dee N, Goldy... NV, Martinez RA, Fong O, Yao S, Mortrud M, Chong P, Loftus L, Bertagnolli D, Goldy J, Casper T, Dee N...BRAIN Armamentarium Collection Caltech Systemic Capsids AAV Packaged on Request AAV Guide Plasmids Publications...

...Boye, S. E., & Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve Transduction and Volumetric...Hurtrel, B., Heard, J.-M., Rivière, Y., & Moullier, P. (2002). Lack of an Immune Response against the Tetracycline-Dependent...refers to the different protein compositions of AAV capsids, which determine how a virus enters the cell. Different...Selective promoters Selective promoters, such as synapsin, glial fibrillary associated protein (GFAP), or...

...subdivide neural expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature...with combinatorial labels. Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet ...schematic at the bottom of this post for a basic synopsis of the differences amongst the Brainbow systems...

... Bohn, P., Joines, K.M., Peng, H., Hawrylycz, M.J., Phillips, J.W., Hohmann, J.G., Wohnoutka, P., Gerfen....L., Kieran, D., Redmond, D.E.Jr., and Aebischer, P. (2010). Efficient transduction of non-human primate... like lentivirus, but instead is contained by a capsid which is comprised of the viral proteins VP1, VP2...genetically engineered to have differences in their capsid proteins. For a brief overview see Addgene’s AAV...

....3389/fmed.2023.1106085. PMID: 36817775. Wilmott, P., Lisowski, L., Alexander, I. E., & Logan, G. J. (...conjunction with the viral rep (replication) and cap (capsid) proteins, which bind to the ITRs and initiate ...containing the AAV2 ITRs can be cross-packaged with capsids from multiple AAV serotypes (Wilmott et al., 2019...2019), and different combinations of ITR and capsid serotypes vary in their gene transfer efficiency. ... 101: Pseudotyping Viral Vectors 101: Systemic Capsids More resource on Addgene.org Addgene's AVV guide...2023). AAV- based vector improvements unrelated to capsid protein modification. Front Med, 10, 1106085. https...

...doi.org/10.1038/s41477-020-0704-5 Santana I, Wu H, Hu P, Giraldo JP (2020) Targeted delivery of nanomaterials...doi.org/10.1038/s41467-020-15731-w Wang F, Wang C, Liu P, Lei C, Hao W, Gao Y, Liu Y-G, Zhao K (2016) Enhanced...including commonly used model organisms like Arabidopsis and Medicago truncatula and several crop species...

...loci in human cells. Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci...chromosomal translocations or chromosomal shattering (chromothripsis). As this study joins the pantheon of useful...

... https://doi.org/10.3389/fnana.2019.00093 Wilmott P, Lisowski L, Alexander IE, Logan GJ (2019) A User’...very high, ubiquitous expression, while the human synapsin 1 promoter is neuronal specific (Haery et al.,...

...Thank you to our guest blogger Derek Simon! Derek P. Simon received his PhD in Cellular and Molecular ... appropriate wavelength to stimulate your opsin. Each opsin has an excitation spectrum (use the resources...materials science-based. Light is delivered to your opsin through a small piece of fiber optic cable implanted...stimulation guarantees that the majority of the opsins will receive sufficient quantities of photons to... otherwise optical stimulation will be erratic, opsin activation/inactivation inconsistent, and behavioral...stimulation         In the video above, Channelrhodopsin 2 (Chr2) has been expressed in the motor cortex...laser must be controlled. As discussed above, each opsin variant has slightly different activation/inactivation...

...Modified pUAST vector containing both P-elements and attB site for high expression...GFP - Retroviral shRNA expression pSico PGK GFP - Conditional shRNA expression...

...Gross-Scherf, B., Morikawa, R.K., Rompani, S.B., Hantz, P., Szikra, T., Esposti, F., Cowan, C.S., Bharioke, ...focused on modifying the outside protein shell, or capsid, of the virus so that it only enters and delivers...the same transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-...

...v3060677 Varanda C, Félix MDR, Campos MD, Materatski P. An Overview of the Application of Viruses to Biotechnology...serotypes (12 naturally occurring and more synthetic capsids are routinely being developed) to choose from, ...