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We narrowed to 60 results for: PSI-P

Showing: 21 - 40 of 60 results
  1. Plant Plasmids and Resources

    Type
    Collection
    ...Physcomitrella patens System for genome editing of the moss P. patens . CRISPR Magdalena Bezanilla 1000000270 ENABLE...seed and other stocks of Arabidopsis thaliana and related species. The Arabidopsis Information Resource (...tools for research using plant systems such as Arabidopsis, wheat, maize, rice, tomato, and a variety of... in variety of plant species (e.g. maize and Arabidopsis) and vectors for easy assembly of one or more...Cas9 assembly for fast generation of multiple Arabidopsis mutants. Engineered biocontainable RNA virus ... on Plant Biology RUBY-Red Siliques Tips for Arabidopsis Transformation Save Time with Transient Plant...Tools for an Old Favorite External Resources Arabidopsis Biological Resource Center (ABRC) (Link opens...
  2. Hot Plasmids: Summer 2025

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    Blog Post
    ...Libraries here! Singh, I., Fernandez-Perez, D., Sanchez, P. S., & Rodriguez-Fraticelli, A. E. (2025). Pre-existing...ATLAS for anterograde synaptic connections By Emily P. Bentley While several viral tools can perform neuronal...TnpB (named ISYmu1) to the popular plant model Arabidopsis (Weiss et al., 2025). By co-delivering TRV1 with...editor for transgene-free germline editing in Arabidopsis. Nature Plants, 11(5), 967–976. https://doi.org...with BioRender.com. Addgene has tested these capsid plasmids in vitro and uses them in our own AAV ...
  3. Hot Plasmids: Winter 2025

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    Blog Post
    ...research   Optimized prime editing in dicots By Emily P. Bentley Prime editing (PE), initially developed in... H., Natale, S., Gomez, L., Taxidis, J., O'Neill, P. S., Villette, V., Bradley, J., Wang, Z., Jiang, D...PE2max-NC and ePEmax3 optimized for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right... to drive prime editing in both tomatoes and Arabidopsis, suggesting the improvements may be broadly applicable...enables heritable desired edits in tomato and Arabidopsis. Nature Plants, 10(10), 1502–1513. doi: https...
  4. Five Popular Model Organisms, Part 2

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    Blog Post
    .... https://doi.org/10.1038/nature02426 Iannaccone, P. M., & Jacob, H. J. (2009). Rats! Disease Models &...Liu, L. S., Zhao, L. Y., Wang, S. H., & Jiang, J. P. (2016). Research proceedings on amphibian model organisms...is as a biomedical model organism.  Arabidopsis thaliana Arabidopsis thaliana is one of, if not the most...community.  The Arabidopsis genome project marked the beginning of widespread Arabidopsis genetics research...characterized genome, and widespread utilization, Arabidopsis has made significant contributions to understanding...understanding plant and crop science alike.  Arabidopsis has many of the traits that make an ideal model organism...maintained in a laboratory environment. What sets Arabidopsis apart from other plant model organisms is its...
  5. 10 Basic tips for mammalian cell culture

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    Blog Post
    ... ER-negative HEK293 cells." PLoS One, 2014. 9(6): p. e98635. PubMed PMID: 24901218. PubMed Central PMCID...2015): 2321-2332. 3. Contamination, Ileana S., John P. S., Basic Methods, Human Stem Cell Manual (Second...PMCID: PMC4047077. 2. Pagliara, Patrizia, et al. "Ostreopsis cf. ovata induces cytoskeletal disorganization...
  6. Common Injection Routes in Mice

    Type
    Blog Post
    ... plasmids Cre-Lox plasmids References Prathipati, P., Rodriguez-Aguayo, C., Walton, B. L., Sood, A. K.....), The Laboratory Mouse. Academic Press. Turner, P. V., Brabb, T., Pekow, C., & Vasbinder, M. A. (2011... place the needle in the correct vein without collapsing it. Retro-orbital injections are also thought...
  7. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post
    ... https://doi.org/10.1089/hum.2008.123 Dhungel, B. P., Xu, H., Nagarajah, R., Vitale, J., Wong, A. C. H... Emily P. Bentley...vectors have both distinctive capsids and genomic structures. The viral capsid is the key that lets AAVs ...of promoter/enhancer. Table 1: AAV capsids and receptors. Capsid Primary receptor Coreceptor AAV1... true! In a previous post, we covered systemic capsids, which allow AAVs to broadly spread throughout ...particular cell type depends largely on the viral capsid, but specificity doesn’t end there: expression ...engineered serotypes by mixing, matching, and modifying capsid and genomic structures. For example, rAAV pseudotypes...
  8. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ..., Public Library of Science (PLoS), 18 Feb. 2011, p. e16765. Crossref, https://doi.org/10.1371/journal.pone...5, Public Library of Science (PLoS), 30 May 2018, p. e0197185. Crossref, https://doi.org/10.1371/journal.pone...Communications, vol. 2, no. 2, Elsevier BV, Mar. 2021, p. 100135. Crossref, https://doi.org/10.1016/j.xplc.2020.100135...Generation of 8× Nicotiana Benthamiana and 12× Arabidopsis Mutants.” The Plant Journal, vol. 106, no. 1,...
  9. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...Boye, S. E., & Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve Transduction and Volumetric...Hurtrel, B., Heard, J.-M., Rivière, Y., & Moullier, P. (2002). Lack of an Immune Response against the Tetracycline-Dependent...refers to the different protein compositions of AAV capsids, which determine how a virus enters the cell. Different...Selective promoters Selective promoters, such as synapsin, glial fibrillary associated protein (GFAP), or...
  10. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...subdivide neural expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature...with combinatorial labels. Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet ...schematic at the bottom of this post for a basic synopsis of the differences amongst the Brainbow systems...
  11. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... Bohn, P., Joines, K.M., Peng, H., Hawrylycz, M.J., Phillips, J.W., Hohmann, J.G., Wohnoutka, P., Gerfen....L., Kieran, D., Redmond, D.E.Jr., and Aebischer, P. (2010). Efficient transduction of non-human primate... like lentivirus, but instead is contained by a capsid which is comprised of the viral proteins VP1, VP2...genetically engineered to have differences in their capsid proteins. For a brief overview see Addgene’s AAV...
  12. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    Blog Post
    ...doi.org/10.1038/s41477-020-0704-5 Santana I, Wu H, Hu P, Giraldo JP (2020) Targeted delivery of nanomaterials...doi.org/10.1038/s41467-020-15731-w Wang F, Wang C, Liu P, Lei C, Hao W, Gao Y, Liu Y-G, Zhao K (2016) Enhanced...including commonly used model organisms like Arabidopsis and Medicago truncatula and several crop species...
  13. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...loci in human cells. Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci...chromosomal translocations or chromosomal shattering (chromothripsis). As this study joins the pantheon of useful...
  14. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    Blog Post
    ... https://doi.org/10.3389/fnana.2019.00093 Wilmott P, Lisowski L, Alexander IE, Logan GJ (2019) A User’...very high, ubiquitous expression, while the human synapsin 1 promoter is neuronal specific (Haery et al.,...
  15. Viral Vectors 101: Inverted Terminal Repeats

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    Blog Post
    ....3389/fmed.2023.1106085. PMID: 36817775. Wilmott, P., Lisowski, L., Alexander, I. E., & Logan, G. J. (...conjunction with the viral rep (replication) and cap (capsid) proteins, which bind to the ITRs and initiate ...containing the AAV2 ITRs can be cross-packaged with capsids from multiple AAV serotypes (Wilmott et al., 2019...2019), and different combinations of ITR and capsid serotypes vary in their gene transfer efficiency. ... 101: Pseudotyping Viral Vectors 101: Systemic Capsids More resource on Addgene.org Addgene's AVV guide...2023). AAV- based vector improvements unrelated to capsid protein modification. Front Med, 10, 1106085. https...
  16. The Materials Science of Optogenetics Experiments

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    Blog Post
    ...Thank you to our guest blogger Derek Simon! Derek P. Simon received his PhD in Cellular and Molecular ... appropriate wavelength to stimulate your opsin. Each opsin has an excitation spectrum (use the resources...materials science-based. Light is delivered to your opsin through a small piece of fiber optic cable implanted...stimulation guarantees that the majority of the opsins will receive sufficient quantities of photons to... otherwise optical stimulation will be erratic, opsin activation/inactivation inconsistent, and behavioral...stimulation         In the video above, Channelrhodopsin 2 (Chr2) has been expressed in the motor cortex...laser must be controlled. As discussed above, each opsin variant has slightly different activation/inactivation...
  17. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...Gross-Scherf, B., Morikawa, R.K., Rompani, S.B., Hantz, P., Szikra, T., Esposti, F., Cowan, C.S., Bharioke, ...focused on modifying the outside protein shell, or capsid, of the virus so that it only enters and delivers...the same transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-...
  18. Viral Vectors 101: Types of viruses

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    Blog Post
    ...v3060677 Varanda C, Félix MDR, Campos MD, Materatski P. An Overview of the Application of Viruses to Biotechnology...serotypes (12 naturally occurring and more synthetic capsids are routinely being developed) to choose from, ...
  19. Viral Production at Addgene

    Type
    Blog Post
    ... element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for normalization...intensity of the viral capsid proteins are analyzed. The abundance of viral capsid proteins as a fraction...the relative stoichiometric ratios of the viral capsid proteins VP1, VP2, and VP3. Samples of viral preparations...non-purified AAV subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are shown relative to...
Showing: 21 - 40 of 60 results