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We narrowed to 741 results for: REV

Showing: 21 - 40 of 741 results

  1. Rett Syndrome

    Type
    Collection
    ...biological progress over 50 years in Rett syndrome. Nat Rev Neurol . 13, 37–51. PMID: 27934853 Neul et al. 2008...RNA trans-splicing, and protein replacement to reverse the impacts of this disease. Addgene is working...typically between 18 to 30 months, is regression of previously acquired skills, notably loss of acquired purposeful... demonstrates that Rett syndrome symptoms are reversible in mice if MECP2 gene expression is switched ... a new window) PMID: 24399845 Guy et al. 2007. Reversal of Neurological Defects in a Mouse Model of Rett...Laurvick et al. 2006. Rett syndrome in Australia: a review of the epidemiology. J Pediatric . 148(3):347-352...PMID: 18337588 Neul et al. 2010. Rett syndrome: revised diagnostic criteria and nomenclature. Ann Neurol...

  2. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ... transcriptional activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different... system is split into two plasmids: one encoding Rev and one encoding Gag and Pol, increasing the number... vectors contain a deletion in the 3’ LTR that prevents aberrant activation of nearby genes. These safer...

  3. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ... human induced pluripotent stem cells. Stem Cell Rev, 2015. 11(2): p. 242-53. PubMed PMID: 25578634. PubMed...reprogramming: three routes to regeneration. Nat Rev Mol Cell Biol, 2011. 12(2): p. 79-89. PubMed PMID... and transdifferentiation are back in style. Nat Rev Mol Cell Biol, 2016. 17(7): p. 413-25. PubMed PMID...via: ReprogrammingA differentiated somatic cell reverts back to a pluripotent state, which then proliferates...pluripotent stage. Cellular reprogramming The reversal of a differentiated cell type to an undifferentiated... - that induce a differentiated somatic cell to revert back to a pluripotent stage (iPSC formation) [2...Trends in Pharmacological Sciences, a Cell Press reviews journal. She joined Cell Press to pursue a career...

  4. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...for CRISPR? A user’s guide to genetic screens. Nat Rev Genet 19:67–80 . https://doi.org/10.1038/nrg.2017.97...the genome and transcriptome of living cells. Nat Rev Genet 19:770–788 . https://doi.org/10.1038/s41576...discussion of these subjects can be found in two recent review articles (Doench et al., 2017, and Hanna et al.... single cell cloning – as a control, one should revert the edit back to the original in order to prove...the window. Another technology, prime editing (reviewed in Anzalone et al., 2020). is not limited to single...

  5. CRISPR History and Development for Genome Engineering

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    Collection
    ...evolutionary classification of CRISPR-Cas systems. Nat Rev Microbiol . 13(11):722-36. PMID: 26411297 Mali P,...gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no signs of slowing down. This prokaryotic...employ varied mechanisms to block CRISPR. Some prevent the CRISPR-Cas complex from binding to DNA. Others...or repressors can modulate transcription in a reversible manner. Epigenetics : dCas9 fused to epigenetic...Plants (monocots and dicots) C. elegans Yeast ( S. cerevisiae and S. pombe ) Zebrafish Xenopus References Barrangou...

  6. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ... CRISPR-Cas in drug development and therapy. Nat Rev Drug Discov (2017) 16(2):89-100. DOI: 10.1038/nrd...they are deeply intertwined. In this blog we will review how CRISPR technology has brought drug and genetic...affect disease progression or onset. These hits can reveal individual genes or sometimes even genetic pathways...proficient and deficient for the target. CRISPR has revolutionized the generation of knockout cell lines which...

  7. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...Deisseroth New serotype (multiple) rAAV-CAG::FLEX-rev:: hM4D-2a-GFP AAV1 Chemogenetics Scott Sternson New...

  9. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...References Robinson HL. Retroviruses and cancer. Rev Infect Dis. 1982 Sep-Oct;4(5):1015-25. DOI: 10.1093...Retroviruses are single-stranded RNA viruses that use reverse transcriptase to synthesize complementary DNA copies...their features Choosing a virus Now that we’ve reviewed the common viruses used in biological research...

  10. All in a Twist: dsRNA

    Type
    Blog Post
    ...of dsRNA, their recognition and consequences. Nat Rev Mol Cell Biol, 23: 286-301 (2022). doi.org/10.1038...pesticides and insecticides, an advancement over previous chemical versions with toxicity to many organisms...

  11. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...natural single-domain antibodies. Annu Rev Biochem. doi: 10.1146/annurev-biochem-063011-092449.            ...

  12. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...further split the viral genome by removing the viral Rev gene from the packaging plasmid and incorporating... lentiviral packaging systems were designed to prevent the formation of replication competent lentivirus...

  14. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...evolutionary classification of CRISPR-Cas systems. 5. Nat Rev Microbiol. 2015 Sep 28. doi: 10.1038/nrmicro3569....elements. To solve this problem, the Zhang lab has previously used SaCas9 (1053 aa/3.2 kb) with AAV vectors...orthologs show lower off-target activity than that previously observed with SpCas9. Kleinstiver et al. suggest... site due its proximity to the cleavage site, preventing future edits. In contrast, since Cpf1 cleaves...mediate HDR in a “second chance” mechanism. To prevent new editing post-HDR, repair templates should remove...Been KW, Yoon SH, Kim JS. Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells...

  15. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...applications in imaging living cells and tissues”  Physiol. Rev. 90:1103. Wu et. al. (2011) “Modern fluorescent proteins...Dendra2, and the mEOS proteins. Some proteins are reversibly switchable (e.g. rsEGFP, Dreiklang). Fluorescent...Plasmids 101: Green Fluorescent Protein (GFP) Three Review Articles on the Different Types of FPs: Stepanenko...

  16. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...Therapeutic Purposes. Annu Rev Virol. 2014 Nov;1(1):427-51. DOI: 10.1146/annurev-virology-031413-085355. ... disease and not another genetic disease more prevalent or life-threatening (cystic fibrosis, hemophilia...manufacturing of viral vectors to support trials for prevalent diseases - to name a few. CRISPR-based tools ...hopefully only the beginning of a gene therapy revolution and the development of personalized medicine.... Vol 526, p.351. PubMed PMID: 26469046. 6. The Forever Fix: Gene Therapy and the Boy Who Saved It by Ricki... Retina. Front Cell Dev Biol. 2018 May 14;6:53. Review. PubMed PMID: 29868583. PubMed Central PMCID: PMC5960719...

  17. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...contribution of synonymous mutations to human disease. Nat Rev Genet 12:683–691. https://doi.org/10.1038/nrg3051...across all endogenous genes in E. coli and S. cerevisiae (Tuller et. al., 2010). Figure 1: An overview...in Natural or synthetic? How Addgene;s dataset reveals trends in biological innovation Resources on Addgene.org...

  18. Viral Production at Addgene

    Type
    Blog Post
    ...probes targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease...titer (protocol modified from Lock et al., 2014.) Previous experience suggests that titers obtained using...

  19. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...evolutionary classification of CRISPR-Cas systems." Nat Rev Microbiol. (2015). PubMed PMID: 26411297. Resources...putative new class 2 nuclease, Cpf1 (CRISPR from Prevotella and Francisella), was annotated in several genomes...site due to its proximity to the cleavage site, preventing future edits. In contrast, since Cpf1 cleaves... has not yet been experimentally confirmed. To prevent new editing post-HDR, repair templates should remove...orthologs show lower off-target activity than that previously observed with SpCas9. Kleinstiver et al. suggest.... 3. Kim, Daesik, et al. Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells...

  20. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...development of pseudoviruses for enveloped viruses. Rev Med Virol 28:e1963 . https://doi.org/10.1002/rmv.1963...For more details, check out this review article published in Reviews in Medical Virology (Li et a., 2018...ultracentrifugation which helps scientists achieve a high titer (reviewed in Joglekar and Sandoval, 2017). This high titer...can be used for specific purposes as described previously (Table 1, Gutierrez-Guerrero, et al, 2020). Because...travel retrogradely, and jump the synapse to the previous neuron (Wichersham et al., 2007). However, once...specific to suit your needs. Check out some of these review articles to find tables and information on different...
Showing: 21 - 40 of 741 results