We narrowed to 100 results for: RFP
-
TypeBlog Post... the leader sequence. Using a variety of GFP and RFP fusions as their test genes, the researchers at the...terminators of varying strengths centered within GFP/RFP bicistronic reporters characterized in Cambray et...
-
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post... the leader sequence. Using a variety of GFP and RFP fusions as their test genes, the researchers at the...terminators of varying strengths centered within GFP/RFP bicistronic reporters characterized in Cambray et... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...cells, each line showed broad recombinase-dependent RFP expression. Even at low expression levels, recombination...Reporter constructs equivalent to site-STOP-site-myrRFP, with “site” representing the specific recombination... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...cells, each line showed broad recombinase-dependent RFP expression. Even at low expression levels, recombination...Reporter constructs equivalent to site-STOP-site-myrRFP, with “site” representing the specific recombination... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog Post...hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain two SfiI restriction... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog Post...hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain two SfiI restriction... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post... imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad filter set, designed for DAPI /...#49004) to the spectra of both mCherry and TagRFP. Here, the TagRFP spectrum is shown in the darker colors...peak of the TagRFP excitation and the emission filter collects a larger fraction of the TagRFP emission ... emission. For this filter set, we would expect TagRFP to give a brighter signal than mCherry. In general...shorter wavelength red fluorescent proteins like TagRFP or mRuby2 than longer wavelength proteins like ... or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent protein such as ... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post... imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad filter set, designed for DAPI /...#49004) to the spectra of both mCherry and TagRFP. Here, the TagRFP spectrum is shown in the darker colors...peak of the TagRFP excitation and the emission filter collects a larger fraction of the TagRFP emission ... emission. For this filter set, we would expect TagRFP to give a brighter signal than mCherry. In general...shorter wavelength red fluorescent proteins like TagRFP or mRuby2 than longer wavelength proteins like ... or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent protein such as ... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...rounds of random mutagenesis screening for enhanced RFP variants. PA-mCherry (E26V / A58T / K69N / L84F /... working on in vivo imaging models perfer the PA-RFPs over PA-GFP. Another class of irreversible PA-FPs...I203R) (2) and PA-mRFP1(S146H / I161V / I197H) (3) were derived from DsRed and mRFP, respectively. When... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...rounds of random mutagenesis screening for enhanced RFP variants. PA-mCherry (E26V / A58T / K69N / L84F /... working on in vivo imaging models perfer the PA-RFPs over PA-GFP. Another class of irreversible PA-FPs...I203R) (2) and PA-mRFP1(S146H / I161V / I197H) (3) were derived from DsRed and mRFP, respectively. When... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...recruits a different fluorescent protein (BFP, GFP, or RFP) to the genomic loci of interest. Pairs of identical... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...recruits a different fluorescent protein (BFP, GFP, or RFP) to the genomic loci of interest. Pairs of identical... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...amounts of non-specific ROS when exposed to YFP and RFP excitation wavelengths, which suggests that it could... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...amounts of non-specific ROS when exposed to YFP and RFP excitation wavelengths, which suggests that it could... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...Walther Mothes 1817 Lamp1-RFP Lysosomes Lamp1 RFP Walther Mothes 79806 pTag-RFP-C-h-Rab11a-c-Myc Recycling... paxillin GFP Rick Horwitz 26720 RFP-zyxin Focal Adhesions Zyxin RFP Anna Huttenlocher 11908 pEGFP-N1 ...AcGFP Gia Voeltz 79802 pTag-RFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagRFP James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc...James Johnson 79800 pTag-RFP-C-h-Rab4a-c-Myc Recycling endosomes Rab4a TagRFP James Johnson 79799 pTag-BFP-C-h-Rab4a-c-Myc...Filaments LifeAct miRFP703 Vladislav Verkhusha 79994 pEB3-miRFP703 Microtubules EB3 miRFP703 Vladislav Verkhusha...GFP Pantelis Tsoulfas 80000 pMito-miRFP703 Mitochondria COX8A miRFP703 Vladislav Verkhusha 36208 pmTurquoise2...H2A mScarlet Dorus Gadella 18982 pHIV-H2BmRFP Chromatin H2B mRFP Bryan Welm, Zena Werb 21045 pH2B_mCherry_IRES_puro2... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog Post...fluorescent proteins: a relatively pH-insensitive RFP variant, DsRed.T3 and a pH-sensitive GFP variant,... -
CRISPRainbow and Genome Visualization
TypeBlog Post...which recruit fluorescent proteins BFP, GFP, and RFP fused to the respective hairpin binding domain. Pairs... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog Post...fluorescent proteins: a relatively pH-insensitive RFP variant, DsRed.T3 and a pH-sensitive GFP variant,... -
CRISPRainbow and Genome Visualization
TypeBlog Post...which recruit fluorescent proteins BFP, GFP, and RFP fused to the respective hairpin binding domain. Pairs... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post...changes in a protein. Cells expressing an RFP reporter plasmid for recombinase activity were exposed...